Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri
The effects of aflatoxin B1 and deoxynivalenol on the luminescence of Vibrio fischeri were investigated to determine the conditions of using the bioluminescence as an indirect means for mycotoxin detection. The culture of Vibrio fischeri showed that bioluminescence reached a peak after 12 hours of incubation at 25 °C and then decreased drastically. During the lag phase which lasted 6 hours, light emission decreased drastically for both the mycotoxin assays - aflatoxin B1 10 µg/ml and deoxynivalenol 20 µg/ml - and the corresponding controls. Distinct bioluminescence inhibition appeared after this period of minimal bioluminescence of the controls and started with the exponential phase of growth. The percentage of bioluminescence inhibition for both mycotoxins was determined after 3.5, 10, 15 and 25 hours of incubation. The bioluminescence of Vibrio fischeri was inhibited with aflatoxin B1 and enhanced with deoxynivalenol. Both effects were delayed and required a long-term incubation over 10 hours, which may help to investigate bioassays for mycotoxin detection.
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Subjects: | Q03 - Contamination et toxicologie alimentaires, mycotoxine, aflatoxine, luminescence, Vibrio, identification, contamination biologique, test biologique, http://aims.fao.org/aos/agrovoc/c_5026, http://aims.fao.org/aos/agrovoc/c_8638, http://aims.fao.org/aos/agrovoc/c_12270, http://aims.fao.org/aos/agrovoc/c_8212, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_28318, http://aims.fao.org/aos/agrovoc/c_15731, |
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dig-cirad-fr-5450332024-01-28T16:03:33Z http://agritrop.cirad.fr/545033/ http://agritrop.cirad.fr/545033/ Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri. Sarter Samira, Metayer Isabelle, Zakhia Nadine. 2008. World Mycotoxin Journal, 1 (2) : 189-193.https://doi.org/10.3920/WMJ2008.1011 <https://doi.org/10.3920/WMJ2008.1011> Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri Sarter, Samira Metayer, Isabelle Zakhia, Nadine eng 2008 World Mycotoxin Journal Q03 - Contamination et toxicologie alimentaires mycotoxine aflatoxine luminescence Vibrio identification contamination biologique test biologique http://aims.fao.org/aos/agrovoc/c_5026 http://aims.fao.org/aos/agrovoc/c_8638 http://aims.fao.org/aos/agrovoc/c_12270 http://aims.fao.org/aos/agrovoc/c_8212 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_28318 http://aims.fao.org/aos/agrovoc/c_15731 The effects of aflatoxin B1 and deoxynivalenol on the luminescence of Vibrio fischeri were investigated to determine the conditions of using the bioluminescence as an indirect means for mycotoxin detection. The culture of Vibrio fischeri showed that bioluminescence reached a peak after 12 hours of incubation at 25 °C and then decreased drastically. During the lag phase which lasted 6 hours, light emission decreased drastically for both the mycotoxin assays - aflatoxin B1 10 µg/ml and deoxynivalenol 20 µg/ml - and the corresponding controls. Distinct bioluminescence inhibition appeared after this period of minimal bioluminescence of the controls and started with the exponential phase of growth. The percentage of bioluminescence inhibition for both mycotoxins was determined after 3.5, 10, 15 and 25 hours of incubation. The bioluminescence of Vibrio fischeri was inhibited with aflatoxin B1 and enhanced with deoxynivalenol. Both effects were delayed and required a long-term incubation over 10 hours, which may help to investigate bioassays for mycotoxin detection. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/545033/1/document_545033.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.3920/WMJ2008.1011 10.3920/WMJ2008.1011 info:eu-repo/semantics/altIdentifier/doi/10.3920/WMJ2008.1011 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3920/WMJ2008.1011 |
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Q03 - Contamination et toxicologie alimentaires mycotoxine aflatoxine luminescence Vibrio identification contamination biologique test biologique http://aims.fao.org/aos/agrovoc/c_5026 http://aims.fao.org/aos/agrovoc/c_8638 http://aims.fao.org/aos/agrovoc/c_12270 http://aims.fao.org/aos/agrovoc/c_8212 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_28318 http://aims.fao.org/aos/agrovoc/c_15731 Q03 - Contamination et toxicologie alimentaires mycotoxine aflatoxine luminescence Vibrio identification contamination biologique test biologique http://aims.fao.org/aos/agrovoc/c_5026 http://aims.fao.org/aos/agrovoc/c_8638 http://aims.fao.org/aos/agrovoc/c_12270 http://aims.fao.org/aos/agrovoc/c_8212 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_28318 http://aims.fao.org/aos/agrovoc/c_15731 |
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Q03 - Contamination et toxicologie alimentaires mycotoxine aflatoxine luminescence Vibrio identification contamination biologique test biologique http://aims.fao.org/aos/agrovoc/c_5026 http://aims.fao.org/aos/agrovoc/c_8638 http://aims.fao.org/aos/agrovoc/c_12270 http://aims.fao.org/aos/agrovoc/c_8212 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_28318 http://aims.fao.org/aos/agrovoc/c_15731 Q03 - Contamination et toxicologie alimentaires mycotoxine aflatoxine luminescence Vibrio identification contamination biologique test biologique http://aims.fao.org/aos/agrovoc/c_5026 http://aims.fao.org/aos/agrovoc/c_8638 http://aims.fao.org/aos/agrovoc/c_12270 http://aims.fao.org/aos/agrovoc/c_8212 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_28318 http://aims.fao.org/aos/agrovoc/c_15731 Sarter, Samira Metayer, Isabelle Zakhia, Nadine Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri |
description |
The effects of aflatoxin B1 and deoxynivalenol on the luminescence of Vibrio fischeri were investigated to determine the conditions of using the bioluminescence as an indirect means for mycotoxin detection. The culture of Vibrio fischeri showed that bioluminescence reached a peak after 12 hours of incubation at 25 °C and then decreased drastically. During the lag phase which lasted 6 hours, light emission decreased drastically for both the mycotoxin assays - aflatoxin B1 10 µg/ml and deoxynivalenol 20 µg/ml - and the corresponding controls. Distinct bioluminescence inhibition appeared after this period of minimal bioluminescence of the controls and started with the exponential phase of growth. The percentage of bioluminescence inhibition for both mycotoxins was determined after 3.5, 10, 15 and 25 hours of incubation. The bioluminescence of Vibrio fischeri was inhibited with aflatoxin B1 and enhanced with deoxynivalenol. Both effects were delayed and required a long-term incubation over 10 hours, which may help to investigate bioassays for mycotoxin detection. |
format |
article |
topic_facet |
Q03 - Contamination et toxicologie alimentaires mycotoxine aflatoxine luminescence Vibrio identification contamination biologique test biologique http://aims.fao.org/aos/agrovoc/c_5026 http://aims.fao.org/aos/agrovoc/c_8638 http://aims.fao.org/aos/agrovoc/c_12270 http://aims.fao.org/aos/agrovoc/c_8212 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_28318 http://aims.fao.org/aos/agrovoc/c_15731 |
author |
Sarter, Samira Metayer, Isabelle Zakhia, Nadine |
author_facet |
Sarter, Samira Metayer, Isabelle Zakhia, Nadine |
author_sort |
Sarter, Samira |
title |
Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri |
title_short |
Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri |
title_full |
Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri |
title_fullStr |
Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri |
title_full_unstemmed |
Effects of mycotoxins, aflatoxin B1 and deoxynivalenol, on the bioluminescence of Vibrio fischeri |
title_sort |
effects of mycotoxins, aflatoxin b1 and deoxynivalenol, on the bioluminescence of vibrio fischeri |
url |
http://agritrop.cirad.fr/545033/ http://agritrop.cirad.fr/545033/1/document_545033.pdf |
work_keys_str_mv |
AT sartersamira effectsofmycotoxinsaflatoxinb1anddeoxynivalenolonthebioluminescenceofvibriofischeri AT metayerisabelle effectsofmycotoxinsaflatoxinb1anddeoxynivalenolonthebioluminescenceofvibriofischeri AT zakhianadine effectsofmycotoxinsaflatoxinb1anddeoxynivalenolonthebioluminescenceofvibriofischeri |
_version_ |
1792496915528548352 |