Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa
Because free-insecticide treated net distribution is planned in Benin (West Africa) during the next few years, we investigated the type, frequency and distribution of insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus mosquitoes in four localities selected on the basis of contrasting agricultural practices, use of insecticides and environment. Bioassays with WHO diagnostic test kits were carried out using pyrethroid, carbamate, organophosphate and organochlorine insecticides. An. gambiae mosquitoes were identified to species and to M or S molecular forms using PCR techniques. Molecular and biochemical assays were carried out to identify kdr and Ace.1 mutations in individual mosquitoes and to detect any increase in the activity of enzymes typically involved in insecticide metabolism (oxidase, esterase and glutathion-S-transférases). WHO diagnostic tests showed high frequency of resistance in An. gambiae and Cx. quinquefasciatus to permethrin and DDT in three areas. This was consistent with the presence of target site insensitivity due to kdr mutation and to increased metabolism through enzymatic activity. Kdr was expressed in both M and S forms. However, less than 1% of An. gambiae or Cx. quiqnuefasciatus showed the presence of the Ace.1R mutation. Carbamate/OP resistance was present at higher frequency in Culex than in An. gambiae. Dieldrin resistance was present in both species at all four localities. A higher frequency of pyrethroid-resistance was found in An. gambiae mosquitoes collected in urban areas compared to those collected in rice growing areas. The expansion of vegetable growing within urban areas probably contributed to selection pressure on mosquitoes. The detection of multiple resistance mechanisms in both An. gambiae and Cx. quinquefasciatus in Benin may represent a threat for the efficacy of ITNs and other forms of vector control such as indoor residual spraying in the future.
Main Authors: | , , , , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | L73 - Maladies des animaux, Anopheles, Culex quinquefasciatus, malaria, vecteur de maladie, contrôle de maladies, résistance aux pesticides, insecticide, carbamate, dieldrine, pyréthrine de syntèse, Anopheles gambiae, http://aims.fao.org/aos/agrovoc/c_462, http://aims.fao.org/aos/agrovoc/c_30517, http://aims.fao.org/aos/agrovoc/c_34312, http://aims.fao.org/aos/agrovoc/c_8164, http://aims.fao.org/aos/agrovoc/c_2327, http://aims.fao.org/aos/agrovoc/c_25427, http://aims.fao.org/aos/agrovoc/c_3887, http://aims.fao.org/aos/agrovoc/c_1296, http://aims.fao.org/aos/agrovoc/c_16178, http://aims.fao.org/aos/agrovoc/c_7575, http://aims.fao.org/aos/agrovoc/c_30508, http://aims.fao.org/aos/agrovoc/c_875, |
Online Access: | http://agritrop.cirad.fr/542622/ http://agritrop.cirad.fr/542622/1/document_542622.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-542622 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5426222024-01-28T15:31:26Z http://agritrop.cirad.fr/542622/ http://agritrop.cirad.fr/542622/ Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa. Corbel Vincent, N'Guessan R.N., Brengues Cécile, Chandre Fabrice, Djogbenou Luc, Martin Thibaud, Akogbeto Martin, Hougard Jean-Marc, Rowland M.. 2007. Acta Tropica, 101 (3) : 207-216.https://doi.org/10.1016/j.actatropica.2007.01.005 <https://doi.org/10.1016/j.actatropica.2007.01.005> Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa Corbel, Vincent N'Guessan, R.N. Brengues, Cécile Chandre, Fabrice Djogbenou, Luc Martin, Thibaud Akogbeto, Martin Hougard, Jean-Marc Rowland, M. eng 2007 Acta Tropica L73 - Maladies des animaux Anopheles Culex quinquefasciatus malaria vecteur de maladie contrôle de maladies résistance aux pesticides insecticide carbamate dieldrine pyréthrine de syntèse Anopheles gambiae http://aims.fao.org/aos/agrovoc/c_462 http://aims.fao.org/aos/agrovoc/c_30517 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_1296 http://aims.fao.org/aos/agrovoc/c_16178 http://aims.fao.org/aos/agrovoc/c_7575 http://aims.fao.org/aos/agrovoc/c_30508 Bénin http://aims.fao.org/aos/agrovoc/c_875 Because free-insecticide treated net distribution is planned in Benin (West Africa) during the next few years, we investigated the type, frequency and distribution of insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus mosquitoes in four localities selected on the basis of contrasting agricultural practices, use of insecticides and environment. Bioassays with WHO diagnostic test kits were carried out using pyrethroid, carbamate, organophosphate and organochlorine insecticides. An. gambiae mosquitoes were identified to species and to M or S molecular forms using PCR techniques. Molecular and biochemical assays were carried out to identify kdr and Ace.1 mutations in individual mosquitoes and to detect any increase in the activity of enzymes typically involved in insecticide metabolism (oxidase, esterase and glutathion-S-transférases). WHO diagnostic tests showed high frequency of resistance in An. gambiae and Cx. quinquefasciatus to permethrin and DDT in three areas. This was consistent with the presence of target site insensitivity due to kdr mutation and to increased metabolism through enzymatic activity. Kdr was expressed in both M and S forms. However, less than 1% of An. gambiae or Cx. quiqnuefasciatus showed the presence of the Ace.1R mutation. Carbamate/OP resistance was present at higher frequency in Culex than in An. gambiae. Dieldrin resistance was present in both species at all four localities. A higher frequency of pyrethroid-resistance was found in An. gambiae mosquitoes collected in urban areas compared to those collected in rice growing areas. The expansion of vegetable growing within urban areas probably contributed to selection pressure on mosquitoes. The detection of multiple resistance mechanisms in both An. gambiae and Cx. quinquefasciatus in Benin may represent a threat for the efficacy of ITNs and other forms of vector control such as indoor residual spraying in the future. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/542622/1/document_542622.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.actatropica.2007.01.005 10.1016/j.actatropica.2007.01.005 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.actatropica.2007.01.005 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.actatropica.2007.01.005 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
L73 - Maladies des animaux Anopheles Culex quinquefasciatus malaria vecteur de maladie contrôle de maladies résistance aux pesticides insecticide carbamate dieldrine pyréthrine de syntèse Anopheles gambiae http://aims.fao.org/aos/agrovoc/c_462 http://aims.fao.org/aos/agrovoc/c_30517 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_1296 http://aims.fao.org/aos/agrovoc/c_16178 http://aims.fao.org/aos/agrovoc/c_7575 http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_875 L73 - Maladies des animaux Anopheles Culex quinquefasciatus malaria vecteur de maladie contrôle de maladies résistance aux pesticides insecticide carbamate dieldrine pyréthrine de syntèse Anopheles gambiae http://aims.fao.org/aos/agrovoc/c_462 http://aims.fao.org/aos/agrovoc/c_30517 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_1296 http://aims.fao.org/aos/agrovoc/c_16178 http://aims.fao.org/aos/agrovoc/c_7575 http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_875 |
spellingShingle |
L73 - Maladies des animaux Anopheles Culex quinquefasciatus malaria vecteur de maladie contrôle de maladies résistance aux pesticides insecticide carbamate dieldrine pyréthrine de syntèse Anopheles gambiae http://aims.fao.org/aos/agrovoc/c_462 http://aims.fao.org/aos/agrovoc/c_30517 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_1296 http://aims.fao.org/aos/agrovoc/c_16178 http://aims.fao.org/aos/agrovoc/c_7575 http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_875 L73 - Maladies des animaux Anopheles Culex quinquefasciatus malaria vecteur de maladie contrôle de maladies résistance aux pesticides insecticide carbamate dieldrine pyréthrine de syntèse Anopheles gambiae http://aims.fao.org/aos/agrovoc/c_462 http://aims.fao.org/aos/agrovoc/c_30517 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_1296 http://aims.fao.org/aos/agrovoc/c_16178 http://aims.fao.org/aos/agrovoc/c_7575 http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_875 Corbel, Vincent N'Guessan, R.N. Brengues, Cécile Chandre, Fabrice Djogbenou, Luc Martin, Thibaud Akogbeto, Martin Hougard, Jean-Marc Rowland, M. Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa |
description |
Because free-insecticide treated net distribution is planned in Benin (West Africa) during the next few years, we investigated the type, frequency and distribution of insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus mosquitoes in four localities selected on the basis of contrasting agricultural practices, use of insecticides and environment. Bioassays with WHO diagnostic test kits were carried out using pyrethroid, carbamate, organophosphate and organochlorine insecticides. An. gambiae mosquitoes were identified to species and to M or S molecular forms using PCR techniques. Molecular and biochemical assays were carried out to identify kdr and Ace.1 mutations in individual mosquitoes and to detect any increase in the activity of enzymes typically involved in insecticide metabolism (oxidase, esterase and glutathion-S-transférases). WHO diagnostic tests showed high frequency of resistance in An. gambiae and Cx. quinquefasciatus to permethrin and DDT in three areas. This was consistent with the presence of target site insensitivity due to kdr mutation and to increased metabolism through enzymatic activity. Kdr was expressed in both M and S forms. However, less than 1% of An. gambiae or Cx. quiqnuefasciatus showed the presence of the Ace.1R mutation. Carbamate/OP resistance was present at higher frequency in Culex than in An. gambiae. Dieldrin resistance was present in both species at all four localities. A higher frequency of pyrethroid-resistance was found in An. gambiae mosquitoes collected in urban areas compared to those collected in rice growing areas. The expansion of vegetable growing within urban areas probably contributed to selection pressure on mosquitoes. The detection of multiple resistance mechanisms in both An. gambiae and Cx. quinquefasciatus in Benin may represent a threat for the efficacy of ITNs and other forms of vector control such as indoor residual spraying in the future. |
format |
article |
topic_facet |
L73 - Maladies des animaux Anopheles Culex quinquefasciatus malaria vecteur de maladie contrôle de maladies résistance aux pesticides insecticide carbamate dieldrine pyréthrine de syntèse Anopheles gambiae http://aims.fao.org/aos/agrovoc/c_462 http://aims.fao.org/aos/agrovoc/c_30517 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_1296 http://aims.fao.org/aos/agrovoc/c_16178 http://aims.fao.org/aos/agrovoc/c_7575 http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_875 |
author |
Corbel, Vincent N'Guessan, R.N. Brengues, Cécile Chandre, Fabrice Djogbenou, Luc Martin, Thibaud Akogbeto, Martin Hougard, Jean-Marc Rowland, M. |
author_facet |
Corbel, Vincent N'Guessan, R.N. Brengues, Cécile Chandre, Fabrice Djogbenou, Luc Martin, Thibaud Akogbeto, Martin Hougard, Jean-Marc Rowland, M. |
author_sort |
Corbel, Vincent |
title |
Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa |
title_short |
Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa |
title_full |
Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa |
title_fullStr |
Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa |
title_full_unstemmed |
Multiple insecticide resistance mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa |
title_sort |
multiple insecticide resistance mechanisms in anopheles gambiae and culex quinquefasciatus from benin, west africa |
url |
http://agritrop.cirad.fr/542622/ http://agritrop.cirad.fr/542622/1/document_542622.pdf |
work_keys_str_mv |
AT corbelvincent multipleinsecticideresistancemechanismsinanophelesgambiaeandculexquinquefasciatusfrombeninwestafrica AT nguessanrn multipleinsecticideresistancemechanismsinanophelesgambiaeandculexquinquefasciatusfrombeninwestafrica AT brenguescecile multipleinsecticideresistancemechanismsinanophelesgambiaeandculexquinquefasciatusfrombeninwestafrica AT chandrefabrice multipleinsecticideresistancemechanismsinanophelesgambiaeandculexquinquefasciatusfrombeninwestafrica AT djogbenouluc multipleinsecticideresistancemechanismsinanophelesgambiaeandculexquinquefasciatusfrombeninwestafrica AT martinthibaud multipleinsecticideresistancemechanismsinanophelesgambiaeandculexquinquefasciatusfrombeninwestafrica AT akogbetomartin multipleinsecticideresistancemechanismsinanophelesgambiaeandculexquinquefasciatusfrombeninwestafrica AT hougardjeanmarc multipleinsecticideresistancemechanismsinanophelesgambiaeandculexquinquefasciatusfrombeninwestafrica AT rowlandm multipleinsecticideresistancemechanismsinanophelesgambiaeandculexquinquefasciatusfrombeninwestafrica |
_version_ |
1792496765110321152 |