Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree
Cassiicolin, a phytotoxin produced by the necrotrophic fungus Corynespora cassiicola, was purified to homogeneity from a rubber tree isolate. The optimized protocol involves reverse phase chromatography followed by size exclusion chromatography, with monitoring of the toxicity on detached rubber tree leaves. Cassiicolin appeared to be a peptide composed of 27 amino acids, glycosylated on the second residue, with a N-terminal pyroglutamic acid and 6 cysteines involved in disulfide bonds. Its molecular mass was estimated to he 2885 Da. No significant sequence homology with other proteins could be found. The availability of pure toxin in sufficient amount is a prerequisite for its structure determination, which is a key step in the understanding of the aggression mechanism. © 2006 Published by Elsevier B.V.
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Subjects: | H20 - Maladies des plantes, U30 - Méthodes de recherche, Corynespora cassiicola, toxine, glycoprotéine, purification, chromatographie, séquence nucléotidique, spectrométrie de masse, Hevea brasiliensis, http://aims.fao.org/aos/agrovoc/c_33337, http://aims.fao.org/aos/agrovoc/c_7828, http://aims.fao.org/aos/agrovoc/c_3307, http://aims.fao.org/aos/agrovoc/c_6377, http://aims.fao.org/aos/agrovoc/c_1594, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_12421, http://aims.fao.org/aos/agrovoc/c_3589, |
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dig-cirad-fr-5387432024-06-27T16:02:14Z http://agritrop.cirad.fr/538743/ http://agritrop.cirad.fr/538743/ Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree. De Lamotte Frédéric, Duviau Marie-Pierre, Sanier Christine, Thai Robert, Poncet Joël, Bieysse Daniel, Breton Frédéric, Pujade-Renaud Valérie. 2007. Journal of Chromatography. B (849) : 357-362.https://doi.org/10.1016/j.jchromb.2006.10.051 <https://doi.org/10.1016/j.jchromb.2006.10.051> Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree De Lamotte, Frédéric Duviau, Marie-Pierre Sanier, Christine Thai, Robert Poncet, Joël Bieysse, Daniel Breton, Frédéric Pujade-Renaud, Valérie eng 2007 Journal of Chromatography. B H20 - Maladies des plantes U30 - Méthodes de recherche Corynespora cassiicola toxine glycoprotéine purification chromatographie séquence nucléotidique spectrométrie de masse Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_33337 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_3307 http://aims.fao.org/aos/agrovoc/c_6377 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_3589 Cassiicolin, a phytotoxin produced by the necrotrophic fungus Corynespora cassiicola, was purified to homogeneity from a rubber tree isolate. The optimized protocol involves reverse phase chromatography followed by size exclusion chromatography, with monitoring of the toxicity on detached rubber tree leaves. Cassiicolin appeared to be a peptide composed of 27 amino acids, glycosylated on the second residue, with a N-terminal pyroglutamic acid and 6 cysteines involved in disulfide bonds. Its molecular mass was estimated to he 2885 Da. No significant sequence homology with other proteins could be found. The availability of pure toxin in sufficient amount is a prerequisite for its structure determination, which is a key step in the understanding of the aggression mechanism. © 2006 Published by Elsevier B.V. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/538743/1/document_538743.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.jchromb.2006.10.051 10.1016/j.jchromb.2006.10.051 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jchromb.2006.10.051 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.jchromb.2006.10.051 |
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H20 - Maladies des plantes U30 - Méthodes de recherche Corynespora cassiicola toxine glycoprotéine purification chromatographie séquence nucléotidique spectrométrie de masse Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_33337 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_3307 http://aims.fao.org/aos/agrovoc/c_6377 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_3589 H20 - Maladies des plantes U30 - Méthodes de recherche Corynespora cassiicola toxine glycoprotéine purification chromatographie séquence nucléotidique spectrométrie de masse Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_33337 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_3307 http://aims.fao.org/aos/agrovoc/c_6377 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_3589 |
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H20 - Maladies des plantes U30 - Méthodes de recherche Corynespora cassiicola toxine glycoprotéine purification chromatographie séquence nucléotidique spectrométrie de masse Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_33337 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_3307 http://aims.fao.org/aos/agrovoc/c_6377 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_3589 H20 - Maladies des plantes U30 - Méthodes de recherche Corynespora cassiicola toxine glycoprotéine purification chromatographie séquence nucléotidique spectrométrie de masse Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_33337 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_3307 http://aims.fao.org/aos/agrovoc/c_6377 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_3589 De Lamotte, Frédéric Duviau, Marie-Pierre Sanier, Christine Thai, Robert Poncet, Joël Bieysse, Daniel Breton, Frédéric Pujade-Renaud, Valérie Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree |
description |
Cassiicolin, a phytotoxin produced by the necrotrophic fungus Corynespora cassiicola, was purified to homogeneity from a rubber tree isolate. The optimized protocol involves reverse phase chromatography followed by size exclusion chromatography, with monitoring of the toxicity on detached rubber tree leaves. Cassiicolin appeared to be a peptide composed of 27 amino acids, glycosylated on the second residue, with a N-terminal pyroglutamic acid and 6 cysteines involved in disulfide bonds. Its molecular mass was estimated to he 2885 Da. No significant sequence homology with other proteins could be found. The availability of pure toxin in sufficient amount is a prerequisite for its structure determination, which is a key step in the understanding of the aggression mechanism. © 2006 Published by Elsevier B.V. |
format |
article |
topic_facet |
H20 - Maladies des plantes U30 - Méthodes de recherche Corynespora cassiicola toxine glycoprotéine purification chromatographie séquence nucléotidique spectrométrie de masse Hevea brasiliensis http://aims.fao.org/aos/agrovoc/c_33337 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_3307 http://aims.fao.org/aos/agrovoc/c_6377 http://aims.fao.org/aos/agrovoc/c_1594 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_3589 |
author |
De Lamotte, Frédéric Duviau, Marie-Pierre Sanier, Christine Thai, Robert Poncet, Joël Bieysse, Daniel Breton, Frédéric Pujade-Renaud, Valérie |
author_facet |
De Lamotte, Frédéric Duviau, Marie-Pierre Sanier, Christine Thai, Robert Poncet, Joël Bieysse, Daniel Breton, Frédéric Pujade-Renaud, Valérie |
author_sort |
De Lamotte, Frédéric |
title |
Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree |
title_short |
Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree |
title_full |
Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree |
title_fullStr |
Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree |
title_full_unstemmed |
Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree |
title_sort |
purification and characterization of cassiicolin, the toxin produced by corynespora cassiicola, causal agent of the leaf fall disease of rubber tree |
url |
http://agritrop.cirad.fr/538743/ http://agritrop.cirad.fr/538743/1/document_538743.pdf |
work_keys_str_mv |
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