Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family
The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. Different isoforms exist in plants but information about their specific role remains to be established. Our purpose is to find out the structural features common to three rice VDAC isoforms and to investigate their (post)transcriptional regulation in response to an osmotic stress. Two new cDNAs encoding mitochondrial VDAC from rice (Oryza sativa) were isolated, sequenced and characterized: a phylogenetic reconstruction permitted identification of orthologues in Poaceae and computer-based analyses predicted 18 transmembrane [bêta]-strands, one amphipathic [alpha]-helix and two different phosphorylation motifs. The expression of three rice vdac genes was investigated. Northern blot analyses indicated that they were expressed in all plant tissues. There was a differential expression of osvdacl and osvdac3, whereas osvdac2 was homogeneously expressed in all tissues. No change in vdac expression was observed under an osmotic stress. However, a fast-enhanced expression of vdac was observed in roots during the recovery period after stress release. This enhanced expression is not correlated to the amount of VDAC protein detected in roots suggesting a posttranscriptional regulation. © 2002 Elsevier Science B.V. All rights reserved. © 2002 Elsevier Science B.V. All rights reserved.
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Subjects: | F30 - Génétique et amélioration des plantes, Oryza sativa, gène, identification, expression des gènes, mitochondrie, échange d'ion, séquence nucléotidique, adn, phylogénie, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_4869, http://aims.fao.org/aos/agrovoc/c_3933, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_13325, |
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dig-cirad-fr-5359892024-01-28T15:00:30Z http://agritrop.cirad.fr/535989/ http://agritrop.cirad.fr/535989/ Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family. Al Bitar Fawaz, Roosens Nancy, Smeyers Mathias, Vauterin Marc, Van Boxtel Jos, Jacobs Michel, Homblé Fabrice. 2003. Biochimica and Biophysica Acta, N. Gene structure and Expression, 1625 : 43-51.https://doi.org/10.1016/S0167-4781(02)00590-0 <https://doi.org/10.1016/S0167-4781(02)00590-0> Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family Al Bitar, Fawaz Roosens, Nancy Smeyers, Mathias Vauterin, Marc Van Boxtel, Jos Jacobs, Michel Homblé, Fabrice eng 2003 Biochimica and Biophysica Acta, N. Gene structure and Expression F30 - Génétique et amélioration des plantes Oryza sativa gène identification expression des gènes mitochondrie échange d'ion séquence nucléotidique adn phylogénie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_4869 http://aims.fao.org/aos/agrovoc/c_3933 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_13325 The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. Different isoforms exist in plants but information about their specific role remains to be established. Our purpose is to find out the structural features common to three rice VDAC isoforms and to investigate their (post)transcriptional regulation in response to an osmotic stress. Two new cDNAs encoding mitochondrial VDAC from rice (Oryza sativa) were isolated, sequenced and characterized: a phylogenetic reconstruction permitted identification of orthologues in Poaceae and computer-based analyses predicted 18 transmembrane [bêta]-strands, one amphipathic [alpha]-helix and two different phosphorylation motifs. The expression of three rice vdac genes was investigated. Northern blot analyses indicated that they were expressed in all plant tissues. There was a differential expression of osvdacl and osvdac3, whereas osvdac2 was homogeneously expressed in all tissues. No change in vdac expression was observed under an osmotic stress. However, a fast-enhanced expression of vdac was observed in roots during the recovery period after stress release. This enhanced expression is not correlated to the amount of VDAC protein detected in roots suggesting a posttranscriptional regulation. © 2002 Elsevier Science B.V. All rights reserved. © 2002 Elsevier Science B.V. All rights reserved. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/535989/1/document_535989.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/S0167-4781(02)00590-0 10.1016/S0167-4781(02)00590-0 info:eu-repo/semantics/altIdentifier/doi/10.1016/S0167-4781(02)00590-0 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/S0167-4781(02)00590-0 |
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F30 - Génétique et amélioration des plantes Oryza sativa gène identification expression des gènes mitochondrie échange d'ion séquence nucléotidique adn phylogénie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_4869 http://aims.fao.org/aos/agrovoc/c_3933 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_13325 F30 - Génétique et amélioration des plantes Oryza sativa gène identification expression des gènes mitochondrie échange d'ion séquence nucléotidique adn phylogénie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_4869 http://aims.fao.org/aos/agrovoc/c_3933 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_13325 |
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F30 - Génétique et amélioration des plantes Oryza sativa gène identification expression des gènes mitochondrie échange d'ion séquence nucléotidique adn phylogénie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_4869 http://aims.fao.org/aos/agrovoc/c_3933 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_13325 F30 - Génétique et amélioration des plantes Oryza sativa gène identification expression des gènes mitochondrie échange d'ion séquence nucléotidique adn phylogénie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_4869 http://aims.fao.org/aos/agrovoc/c_3933 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_13325 Al Bitar, Fawaz Roosens, Nancy Smeyers, Mathias Vauterin, Marc Van Boxtel, Jos Jacobs, Michel Homblé, Fabrice Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family |
description |
The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. Different isoforms exist in plants but information about their specific role remains to be established. Our purpose is to find out the structural features common to three rice VDAC isoforms and to investigate their (post)transcriptional regulation in response to an osmotic stress. Two new cDNAs encoding mitochondrial VDAC from rice (Oryza sativa) were isolated, sequenced and characterized: a phylogenetic reconstruction permitted identification of orthologues in Poaceae and computer-based analyses predicted 18 transmembrane [bêta]-strands, one amphipathic [alpha]-helix and two different phosphorylation motifs. The expression of three rice vdac genes was investigated. Northern blot analyses indicated that they were expressed in all plant tissues. There was a differential expression of osvdacl and osvdac3, whereas osvdac2 was homogeneously expressed in all tissues. No change in vdac expression was observed under an osmotic stress. However, a fast-enhanced expression of vdac was observed in roots during the recovery period after stress release. This enhanced expression is not correlated to the amount of VDAC protein detected in roots suggesting a posttranscriptional regulation. © 2002 Elsevier Science B.V. All rights reserved. © 2002 Elsevier Science B.V. All rights reserved. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Oryza sativa gène identification expression des gènes mitochondrie échange d'ion séquence nucléotidique adn phylogénie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_4869 http://aims.fao.org/aos/agrovoc/c_3933 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_13325 |
author |
Al Bitar, Fawaz Roosens, Nancy Smeyers, Mathias Vauterin, Marc Van Boxtel, Jos Jacobs, Michel Homblé, Fabrice |
author_facet |
Al Bitar, Fawaz Roosens, Nancy Smeyers, Mathias Vauterin, Marc Van Boxtel, Jos Jacobs, Michel Homblé, Fabrice |
author_sort |
Al Bitar, Fawaz |
title |
Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family |
title_short |
Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family |
title_full |
Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family |
title_fullStr |
Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family |
title_full_unstemmed |
Sequence analysis, transcriptional and posttranscriptional regulation of the rice VDAC family |
title_sort |
sequence analysis, transcriptional and posttranscriptional regulation of the rice vdac family |
url |
http://agritrop.cirad.fr/535989/ http://agritrop.cirad.fr/535989/1/document_535989.pdf |
work_keys_str_mv |
AT albitarfawaz sequenceanalysistranscriptionalandposttranscriptionalregulationofthericevdacfamily AT roosensnancy sequenceanalysistranscriptionalandposttranscriptionalregulationofthericevdacfamily AT smeyersmathias sequenceanalysistranscriptionalandposttranscriptionalregulationofthericevdacfamily AT vauterinmarc sequenceanalysistranscriptionalandposttranscriptionalregulationofthericevdacfamily AT vanboxteljos sequenceanalysistranscriptionalandposttranscriptionalregulationofthericevdacfamily AT jacobsmichel sequenceanalysistranscriptionalandposttranscriptionalregulationofthericevdacfamily AT homblefabrice sequenceanalysistranscriptionalandposttranscriptionalregulationofthericevdacfamily |
_version_ |
1792496598127738880 |