Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus : editing of the gene transcript
The gene encoding the phosphoprotein of the vaccine strain of Peste des petits ruminants (PPR) virus (Nigeria 75/1 vaccine strain) has been cloned and its nucleotide sequence been determined. This gene is 1655 nucleotides long and encodes two overlapping open reading frames (ORFs). Translation from the first AUG would produce a polypeptide of 509 amino acid residues with a predicted molecular mass of 54.9 kDa, the longest of the published morbillivirus P proteins. Translation from the second AUG would produce a protein of 177 amino acid residues with a predicted molecular mass of 20.3 kDa, analogous to the C proteins of other morbilliviruses. Evidence was found for the production of two types of P mRNA transcript, one a faithful transcript of the gene and the other with an extra G residue inserted at position 751. Translation from the first AUG of this second mRNA would produce a protein of 298 amino acids, with a predicted molecular mass 32.3 kDa, analogous to the V protein produced by other morbilliviruses. Sequences of the predicted P, C and V proteins were compared with those of the other morbillivirus sequences available to date. The P protein was found to be the most poorly conserved of the morbillivirus proteins, the amino acid identity ranging from 54% in case of Canine distemper virus (CDV) to 60% in the case of the Dolphin morbillivirus (DMV). © 2003 Elsevier B.V. All rights reserved.
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Subjects: | L50 - Physiologie et biochimie animales, L73 - Maladies des animaux, morbillivirus, phosphoprotéine, peste des petits ruminants, virus, analyse biologique, http://aims.fao.org/aos/agrovoc/c_4937, http://aims.fao.org/aos/agrovoc/c_26871, http://aims.fao.org/aos/agrovoc/c_16789, http://aims.fao.org/aos/agrovoc/c_8262, http://aims.fao.org/aos/agrovoc/c_15944, |
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dig-cirad-fr-5354562024-01-28T14:56:47Z http://agritrop.cirad.fr/535456/ http://agritrop.cirad.fr/535456/ Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus : editing of the gene transcript. Mahapatra Madhuchhanda, Parida Satya, Egziabher Berhe G., Diallo Adama, Barrett Tom. 2003. Virus Research, 96 : 85-98.https://doi.org/10.1016/S0168-1702(03)00176-X <https://doi.org/10.1016/S0168-1702(03)00176-X> Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus : editing of the gene transcript Mahapatra, Madhuchhanda Parida, Satya Egziabher, Berhe G. Diallo, Adama Barrett, Tom eng 2003 Virus Research L50 - Physiologie et biochimie animales L73 - Maladies des animaux morbillivirus phosphoprotéine peste des petits ruminants virus analyse biologique http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_26871 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_8262 http://aims.fao.org/aos/agrovoc/c_15944 The gene encoding the phosphoprotein of the vaccine strain of Peste des petits ruminants (PPR) virus (Nigeria 75/1 vaccine strain) has been cloned and its nucleotide sequence been determined. This gene is 1655 nucleotides long and encodes two overlapping open reading frames (ORFs). Translation from the first AUG would produce a polypeptide of 509 amino acid residues with a predicted molecular mass of 54.9 kDa, the longest of the published morbillivirus P proteins. Translation from the second AUG would produce a protein of 177 amino acid residues with a predicted molecular mass of 20.3 kDa, analogous to the C proteins of other morbilliviruses. Evidence was found for the production of two types of P mRNA transcript, one a faithful transcript of the gene and the other with an extra G residue inserted at position 751. Translation from the first AUG of this second mRNA would produce a protein of 298 amino acids, with a predicted molecular mass 32.3 kDa, analogous to the V protein produced by other morbilliviruses. Sequences of the predicted P, C and V proteins were compared with those of the other morbillivirus sequences available to date. The P protein was found to be the most poorly conserved of the morbillivirus proteins, the amino acid identity ranging from 54% in case of Canine distemper virus (CDV) to 60% in the case of the Dolphin morbillivirus (DMV). © 2003 Elsevier B.V. All rights reserved. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/535456/1/document_535456.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/S0168-1702(03)00176-X 10.1016/S0168-1702(03)00176-X http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=194336 info:eu-repo/semantics/altIdentifier/doi/10.1016/S0168-1702(03)00176-X info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/S0168-1702(03)00176-X |
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L50 - Physiologie et biochimie animales L73 - Maladies des animaux morbillivirus phosphoprotéine peste des petits ruminants virus analyse biologique http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_26871 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_8262 http://aims.fao.org/aos/agrovoc/c_15944 L50 - Physiologie et biochimie animales L73 - Maladies des animaux morbillivirus phosphoprotéine peste des petits ruminants virus analyse biologique http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_26871 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_8262 http://aims.fao.org/aos/agrovoc/c_15944 |
spellingShingle |
L50 - Physiologie et biochimie animales L73 - Maladies des animaux morbillivirus phosphoprotéine peste des petits ruminants virus analyse biologique http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_26871 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_8262 http://aims.fao.org/aos/agrovoc/c_15944 L50 - Physiologie et biochimie animales L73 - Maladies des animaux morbillivirus phosphoprotéine peste des petits ruminants virus analyse biologique http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_26871 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_8262 http://aims.fao.org/aos/agrovoc/c_15944 Mahapatra, Madhuchhanda Parida, Satya Egziabher, Berhe G. Diallo, Adama Barrett, Tom Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus : editing of the gene transcript |
description |
The gene encoding the phosphoprotein of the vaccine strain of Peste des petits ruminants (PPR) virus (Nigeria 75/1 vaccine strain) has been cloned and its nucleotide sequence been determined. This gene is 1655 nucleotides long and encodes two overlapping open reading frames (ORFs). Translation from the first AUG would produce a polypeptide of 509 amino acid residues with a predicted molecular mass of 54.9 kDa, the longest of the published morbillivirus P proteins. Translation from the second AUG would produce a protein of 177 amino acid residues with a predicted molecular mass of 20.3 kDa, analogous to the C proteins of other morbilliviruses. Evidence was found for the production of two types of P mRNA transcript, one a faithful transcript of the gene and the other with an extra G residue inserted at position 751. Translation from the first AUG of this second mRNA would produce a protein of 298 amino acids, with a predicted molecular mass 32.3 kDa, analogous to the V protein produced by other morbilliviruses. Sequences of the predicted P, C and V proteins were compared with those of the other morbillivirus sequences available to date. The P protein was found to be the most poorly conserved of the morbillivirus proteins, the amino acid identity ranging from 54% in case of Canine distemper virus (CDV) to 60% in the case of the Dolphin morbillivirus (DMV). © 2003 Elsevier B.V. All rights reserved. |
format |
article |
topic_facet |
L50 - Physiologie et biochimie animales L73 - Maladies des animaux morbillivirus phosphoprotéine peste des petits ruminants virus analyse biologique http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_26871 http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_8262 http://aims.fao.org/aos/agrovoc/c_15944 |
author |
Mahapatra, Madhuchhanda Parida, Satya Egziabher, Berhe G. Diallo, Adama Barrett, Tom |
author_facet |
Mahapatra, Madhuchhanda Parida, Satya Egziabher, Berhe G. Diallo, Adama Barrett, Tom |
author_sort |
Mahapatra, Madhuchhanda |
title |
Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus : editing of the gene transcript |
title_short |
Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus : editing of the gene transcript |
title_full |
Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus : editing of the gene transcript |
title_fullStr |
Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus : editing of the gene transcript |
title_full_unstemmed |
Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus : editing of the gene transcript |
title_sort |
sequence analysis of the phosphoprotein gene of peste des petits ruminants (ppr) virus : editing of the gene transcript |
url |
http://agritrop.cirad.fr/535456/ http://agritrop.cirad.fr/535456/1/document_535456.pdf |
work_keys_str_mv |
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