Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants
An efficient procedure for producing transgenic Hevea brasiliensis callus and plant lines from clone PB 260 was established with Agrobacterium tumefaciens using strain EHA 105 harbouring the vector pCAMB1A230I . Transformation capacity and competence of the embryo-genic calli were improved after two cycles of cryopreservation. When the cocultivation temperature was reduced from 27 to 20°C, the duration of this phase could be increased up to 7 days, promoting an increase in GUS activity. These transformation conditions led to the isolation of 24 callus lines resistant to paromomycin, which is used as a selection agent. Nineteen of these lines revealed the existence of one to four copies of T-DNA by Southern-blot analysis. Nine of them were transferred for regeneration by somatic embryo-genesis. Three hundred seventy-four transgenic plants have thus been generated from six independent lines bearing 1, 2 or 3 copies of T-DNA. The efficiency and reproducibility of this method means that functional characterization of genes involved in natural rubber production can be envisaged.
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Language: | eng |
Subjects: | F30 - Génétique et amélioration des plantes, Hevea brasiliensis, Agrobacterium tumefaciens, transformation génétique, Embryogénèse somatique, antibiotique, plante transgénique, séquence nucléotidique, adn, génie génétique, cryoconservation, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_27444, http://aims.fao.org/aos/agrovoc/c_3220, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_492, http://aims.fao.org/aos/agrovoc/c_27619, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_15974, http://aims.fao.org/aos/agrovoc/c_24155, |
Online Access: | http://agritrop.cirad.fr/530928/ http://agritrop.cirad.fr/530928/1/document_530928.pdf |
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dig-cirad-fr-5309282024-01-28T14:19:05Z http://agritrop.cirad.fr/530928/ http://agritrop.cirad.fr/530928/ Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants. Blanc Géraldine, Baptiste Christelle, Oliver Gérald, Martin Florence, Montoro Pascal. 2006. Plant Cell Reports, 24 (12) : 724-733.https://doi.org/10.1007/s00299-005-0023-3 <https://doi.org/10.1007/s00299-005-0023-3> Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants Blanc, Géraldine Baptiste, Christelle Oliver, Gérald Martin, Florence Montoro, Pascal eng 2006 Plant Cell Reports F30 - Génétique et amélioration des plantes Hevea brasiliensis Agrobacterium tumefaciens transformation génétique Embryogénèse somatique antibiotique plante transgénique séquence nucléotidique adn génie génétique cryoconservation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_24155 An efficient procedure for producing transgenic Hevea brasiliensis callus and plant lines from clone PB 260 was established with Agrobacterium tumefaciens using strain EHA 105 harbouring the vector pCAMB1A230I . Transformation capacity and competence of the embryo-genic calli were improved after two cycles of cryopreservation. When the cocultivation temperature was reduced from 27 to 20°C, the duration of this phase could be increased up to 7 days, promoting an increase in GUS activity. These transformation conditions led to the isolation of 24 callus lines resistant to paromomycin, which is used as a selection agent. Nineteen of these lines revealed the existence of one to four copies of T-DNA by Southern-blot analysis. Nine of them were transferred for regeneration by somatic embryo-genesis. Three hundred seventy-four transgenic plants have thus been generated from six independent lines bearing 1, 2 or 3 copies of T-DNA. The efficiency and reproducibility of this method means that functional characterization of genes involved in natural rubber production can be envisaged. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/530928/1/document_530928.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s00299-005-0023-3 10.1007/s00299-005-0023-3 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00299-005-0023-3 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s00299-005-0023-3 |
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F30 - Génétique et amélioration des plantes Hevea brasiliensis Agrobacterium tumefaciens transformation génétique Embryogénèse somatique antibiotique plante transgénique séquence nucléotidique adn génie génétique cryoconservation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_24155 F30 - Génétique et amélioration des plantes Hevea brasiliensis Agrobacterium tumefaciens transformation génétique Embryogénèse somatique antibiotique plante transgénique séquence nucléotidique adn génie génétique cryoconservation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_24155 |
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F30 - Génétique et amélioration des plantes Hevea brasiliensis Agrobacterium tumefaciens transformation génétique Embryogénèse somatique antibiotique plante transgénique séquence nucléotidique adn génie génétique cryoconservation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_24155 F30 - Génétique et amélioration des plantes Hevea brasiliensis Agrobacterium tumefaciens transformation génétique Embryogénèse somatique antibiotique plante transgénique séquence nucléotidique adn génie génétique cryoconservation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_24155 Blanc, Géraldine Baptiste, Christelle Oliver, Gérald Martin, Florence Montoro, Pascal Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants |
description |
An efficient procedure for producing transgenic Hevea brasiliensis callus and plant lines from clone PB 260 was established with Agrobacterium tumefaciens using strain EHA 105 harbouring the vector pCAMB1A230I . Transformation capacity and competence of the embryo-genic calli were improved after two cycles of cryopreservation. When the cocultivation temperature was reduced from 27 to 20°C, the duration of this phase could be increased up to 7 days, promoting an increase in GUS activity. These transformation conditions led to the isolation of 24 callus lines resistant to paromomycin, which is used as a selection agent. Nineteen of these lines revealed the existence of one to four copies of T-DNA by Southern-blot analysis. Nine of them were transferred for regeneration by somatic embryo-genesis. Three hundred seventy-four transgenic plants have thus been generated from six independent lines bearing 1, 2 or 3 copies of T-DNA. The efficiency and reproducibility of this method means that functional characterization of genes involved in natural rubber production can be envisaged. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Hevea brasiliensis Agrobacterium tumefaciens transformation génétique Embryogénèse somatique antibiotique plante transgénique séquence nucléotidique adn génie génétique cryoconservation http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_24155 |
author |
Blanc, Géraldine Baptiste, Christelle Oliver, Gérald Martin, Florence Montoro, Pascal |
author_facet |
Blanc, Géraldine Baptiste, Christelle Oliver, Gérald Martin, Florence Montoro, Pascal |
author_sort |
Blanc, Géraldine |
title |
Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants |
title_short |
Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants |
title_full |
Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants |
title_fullStr |
Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants |
title_full_unstemmed |
Efficient Agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of Hevea brasiliensis Müll Arg. plants |
title_sort |
efficient agrobacterium tumefaciens-mediated transformation of embryogenic calli and regeneration of hevea brasiliensis müll arg. plants |
url |
http://agritrop.cirad.fr/530928/ http://agritrop.cirad.fr/530928/1/document_530928.pdf |
work_keys_str_mv |
AT blancgeraldine efficientagrobacteriumtumefaciensmediatedtransformationofembryogeniccalliandregenerationofheveabrasiliensismullargplants AT baptistechristelle efficientagrobacteriumtumefaciensmediatedtransformationofembryogeniccalliandregenerationofheveabrasiliensismullargplants AT olivergerald efficientagrobacteriumtumefaciensmediatedtransformationofembryogeniccalliandregenerationofheveabrasiliensismullargplants AT martinflorence efficientagrobacteriumtumefaciensmediatedtransformationofembryogeniccalliandregenerationofheveabrasiliensismullargplants AT montoropascal efficientagrobacteriumtumefaciensmediatedtransformationofembryogeniccalliandregenerationofheveabrasiliensismullargplants |
_version_ |
1792496390582042624 |