Assessing the genetic diversity in the International cocoa genebank, trinidad (ICG, T) using isozyme electrophoresis and RAPD

Random amplified polymorphic DNA (RAPD) and isozyme electrophoresis (IE) techniques were used to estimate the level of genetic diversity in a sample of cacao germplasm existing at the International Cocoa Genebank, Trinidad. Twenty-six cocoa populations represented by 459 cocoa genotypes were analysed using IE and 22 populations represented by 353 cocoa genotypes were analysed using RAPD. Despite few differences in the classification of the populations, both techniques revealed three major groups: the indigenous trees, the cultivated Trinitario and the cultivated trees from Ecuador. Two-thirds of the partitioned diversity were found within populations and one-third between the populations, with both techniques.

Bibliographic Details

Main Authors:	Sounigo, Olivier, Umaharan, Romina, Christopher, Yvette, Sankar, Antoinette, Ramdahin, Stacy
Format:	article biblioteca
Language:	eng
Published:	Springer
Subjects:	F30 - Génétique et amélioration des plantes, U30 - Méthodes de recherche, Theobroma cacao, http://aims.fao.org/aos/agrovoc/c_7713, http://aims.fao.org/aos/agrovoc/c_7933,
Online Access:	http://agritrop.cirad.fr/530729/ http://agritrop.cirad.fr/530729/1/530729.pdf
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