Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure
Optimization of protoplast fusion parameters is a prerequisite for the establishment of somatic fusion technology for banana breeding. In the present investigations, we compared the most frequently used fusion methods: the electrofusion technique and chemical procedure (polyethylene glycol). With regard to frequency of binary fusion, protoplast fusion with the fusogen polyethylene glycol was best. Conversely, electric fusion was found to be better with respect to mitotic activities, somatic embryogenesis and plantlet regeneration rate.
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dig-cirad-fr-5291482022-12-30T18:27:28Z http://agritrop.cirad.fr/529148/ http://agritrop.cirad.fr/529148/ Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure. Assani Akym, Chabane Djamila, Haïcour Robert, Bakry Frédéric, Wenzel Gerhard, Foroughi-Wehr Barbel. 2005. Plant Cell, Tissue and Organ Culture, 83 (2) : 145-151.https://doi.org/10.1007/s11240-005-4633-9 <https://doi.org/10.1007/s11240-005-4633-9> Researchers Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure Assani, Akym Chabane, Djamila Haïcour, Robert Bakry, Frédéric Wenzel, Gerhard Foroughi-Wehr, Barbel eng 2005 Plant Cell, Tissue and Organ Culture F30 - Génétique et amélioration des plantes Optimization of protoplast fusion parameters is a prerequisite for the establishment of somatic fusion technology for banana breeding. In the present investigations, we compared the most frequently used fusion methods: the electrofusion technique and chemical procedure (polyethylene glycol). With regard to frequency of binary fusion, protoplast fusion with the fusogen polyethylene glycol was best. Conversely, electric fusion was found to be better with respect to mitotic activities, somatic embryogenesis and plantlet regeneration rate. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/529148/1/document_529148.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s11240-005-4633-9 10.1007/s11240-005-4633-9 info:eu-repo/semantics/altIdentifier/doi/10.1007/s11240-005-4633-9 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s11240-005-4633-9 |
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F30 - Génétique et amélioration des plantes F30 - Génétique et amélioration des plantes Assani, Akym Chabane, Djamila Haïcour, Robert Bakry, Frédéric Wenzel, Gerhard Foroughi-Wehr, Barbel Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure |
| description |
Optimization of protoplast fusion parameters is a prerequisite for the establishment of somatic fusion technology for banana breeding. In the present investigations, we compared the most frequently used fusion methods: the electrofusion technique and chemical procedure (polyethylene glycol). With regard to frequency of binary fusion, protoplast fusion with the fusogen polyethylene glycol was best. Conversely, electric fusion was found to be better with respect to mitotic activities, somatic embryogenesis and plantlet regeneration rate. |
| format |
article |
| topic_facet |
F30 - Génétique et amélioration des plantes |
| author |
Assani, Akym Chabane, Djamila Haïcour, Robert Bakry, Frédéric Wenzel, Gerhard Foroughi-Wehr, Barbel |
| author_facet |
Assani, Akym Chabane, Djamila Haïcour, Robert Bakry, Frédéric Wenzel, Gerhard Foroughi-Wehr, Barbel |
| author_sort |
Assani, Akym |
| title |
Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure |
| title_short |
Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure |
| title_full |
Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure |
| title_fullStr |
Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure |
| title_full_unstemmed |
Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure |
| title_sort |
protoplast fusion in banana (musa spp.) : comparison of chemical (peg: polyethylene glycol) and electrical procedure |
| url |
http://agritrop.cirad.fr/529148/ http://agritrop.cirad.fr/529148/1/document_529148.pdf |
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