High genetic variability in an RNA plant virus, banana mild mosaic virus
Background: Banana Mild Mosaic virus (BanMMV) is an unassigned member of the Flexiviridae family infecting banana and plantain (Musa spp). Vertical transmission by vegetative propagation is currently the only known means of propagation of this virus, since no biological vector has been identified nor has experimental mechanical inoculation on susceptible hosts been successful so far. Methods: Nucleotide sequences were obtained from BanMMV isolates originating from various locations worldwide. Two distinct regions of the viral genome were targeted: ORF 1, which encodes the viral RNA-dependent RNA polymerase (RdRp), and a region encompassing the 3' end of ORF5, which encodes the coat protein (CP), and the 3' non-coding region (3' NCR). Both regions play a key role in viral replication and are therefore supposedly very conserved. Results: BanMMV RdRp sequences displayed an extremely high level of molecular diversity when compared to similar sequences obtained from other plant RNA viruses. This situation resulted essentially from a very high ratio of synonymous mutations. A similar analysis performed for a limited selection of banana accessions on the CP/3'NCR region confirmed the high level of diversity observed in the RdRp dataset, characterized by a very high level of synonymous mutations. Analysis of intra-host diversity indicated the existence of two distinct situations with some plants harbouring only closely related sequence variants while others contained up to three widely divergent isolates. Analyses indicated an absence of structuration of the virus genetic diversity either by the geographical origin of the infected Musa accessions or by their genotype. This situation may be, in part, explained by the exchange of banana germplasm between different parts of the world and also by plant-to-plant transmission of the virus, the evidence for which is, for the first time, provided by this study.
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Subjects: | F30 - Génétique et amélioration des plantes, H20 - Maladies des plantes, Musa, virus des végétaux, variation génétique, ressource génétique, arn, transmission des maladies, multiplication des plantes, vecteur de maladie, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_3218, http://aims.fao.org/aos/agrovoc/c_6618, http://aims.fao.org/aos/agrovoc/c_2329, http://aims.fao.org/aos/agrovoc/c_5977, http://aims.fao.org/aos/agrovoc/c_8164, |
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dig-cirad-fr-5277122024-01-28T13:49:49Z http://agritrop.cirad.fr/527712/ http://agritrop.cirad.fr/527712/ High genetic variability in an RNA plant virus, banana mild mosaic virus. Teycheney Pierre-Yves, Laboureau Nathalie, Iskra Caruana Marie-Line, Candresse Thierry. 2005. In : Microbes in a changing world = XIIIth International Congress of Virology, San Fransisco (USA), July 23-28, 2005. s.l. : s.n., 2 p. International Congress of Virology. 13, San Francisco, États-Unis, 23 Juillet 2005/28 Juillet 2005. High genetic variability in an RNA plant virus, banana mild mosaic virus Teycheney, Pierre-Yves Laboureau, Nathalie Iskra Caruana, Marie-Line Candresse, Thierry eng 2005 s.n. Microbes in a changing world = XIIIth International Congress of Virology, San Fransisco (USA), July 23-28, 2005 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Musa virus des végétaux variation génétique ressource génétique arn transmission des maladies multiplication des plantes vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_3218 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5977 http://aims.fao.org/aos/agrovoc/c_8164 Background: Banana Mild Mosaic virus (BanMMV) is an unassigned member of the Flexiviridae family infecting banana and plantain (Musa spp). Vertical transmission by vegetative propagation is currently the only known means of propagation of this virus, since no biological vector has been identified nor has experimental mechanical inoculation on susceptible hosts been successful so far. Methods: Nucleotide sequences were obtained from BanMMV isolates originating from various locations worldwide. Two distinct regions of the viral genome were targeted: ORF 1, which encodes the viral RNA-dependent RNA polymerase (RdRp), and a region encompassing the 3' end of ORF5, which encodes the coat protein (CP), and the 3' non-coding region (3' NCR). Both regions play a key role in viral replication and are therefore supposedly very conserved. Results: BanMMV RdRp sequences displayed an extremely high level of molecular diversity when compared to similar sequences obtained from other plant RNA viruses. This situation resulted essentially from a very high ratio of synonymous mutations. A similar analysis performed for a limited selection of banana accessions on the CP/3'NCR region confirmed the high level of diversity observed in the RdRp dataset, characterized by a very high level of synonymous mutations. Analysis of intra-host diversity indicated the existence of two distinct situations with some plants harbouring only closely related sequence variants while others contained up to three widely divergent isolates. Analyses indicated an absence of structuration of the virus genetic diversity either by the geographical origin of the infected Musa accessions or by their genotype. This situation may be, in part, explained by the exchange of banana germplasm between different parts of the world and also by plant-to-plant transmission of the virus, the evidence for which is, for the first time, provided by this study. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/527712/1/document_527712.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=187416 |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Musa virus des végétaux variation génétique ressource génétique arn transmission des maladies multiplication des plantes vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_3218 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5977 http://aims.fao.org/aos/agrovoc/c_8164 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Musa virus des végétaux variation génétique ressource génétique arn transmission des maladies multiplication des plantes vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_3218 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5977 http://aims.fao.org/aos/agrovoc/c_8164 |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Musa virus des végétaux variation génétique ressource génétique arn transmission des maladies multiplication des plantes vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_3218 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5977 http://aims.fao.org/aos/agrovoc/c_8164 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Musa virus des végétaux variation génétique ressource génétique arn transmission des maladies multiplication des plantes vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_3218 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5977 http://aims.fao.org/aos/agrovoc/c_8164 Teycheney, Pierre-Yves Laboureau, Nathalie Iskra Caruana, Marie-Line Candresse, Thierry High genetic variability in an RNA plant virus, banana mild mosaic virus |
description |
Background: Banana Mild Mosaic virus (BanMMV) is an unassigned member of the Flexiviridae family infecting banana and plantain (Musa spp). Vertical transmission by vegetative propagation is currently the only known means of propagation of this virus, since no biological vector has been identified nor has experimental mechanical inoculation on susceptible hosts been successful so far. Methods: Nucleotide sequences were obtained from BanMMV isolates originating from various locations worldwide. Two distinct regions of the viral genome were targeted: ORF 1, which encodes the viral RNA-dependent RNA polymerase (RdRp), and a region encompassing the 3' end of ORF5, which encodes the coat protein (CP), and the 3' non-coding region (3' NCR). Both regions play a key role in viral replication and are therefore supposedly very conserved. Results: BanMMV RdRp sequences displayed an extremely high level of molecular diversity when compared to similar sequences obtained from other plant RNA viruses. This situation resulted essentially from a very high ratio of synonymous mutations. A similar analysis performed for a limited selection of banana accessions on the CP/3'NCR region confirmed the high level of diversity observed in the RdRp dataset, characterized by a very high level of synonymous mutations. Analysis of intra-host diversity indicated the existence of two distinct situations with some plants harbouring only closely related sequence variants while others contained up to three widely divergent isolates. Analyses indicated an absence of structuration of the virus genetic diversity either by the geographical origin of the infected Musa accessions or by their genotype. This situation may be, in part, explained by the exchange of banana germplasm between different parts of the world and also by plant-to-plant transmission of the virus, the evidence for which is, for the first time, provided by this study. |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Musa virus des végétaux variation génétique ressource génétique arn transmission des maladies multiplication des plantes vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_3218 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5977 http://aims.fao.org/aos/agrovoc/c_8164 |
author |
Teycheney, Pierre-Yves Laboureau, Nathalie Iskra Caruana, Marie-Line Candresse, Thierry |
author_facet |
Teycheney, Pierre-Yves Laboureau, Nathalie Iskra Caruana, Marie-Line Candresse, Thierry |
author_sort |
Teycheney, Pierre-Yves |
title |
High genetic variability in an RNA plant virus, banana mild mosaic virus |
title_short |
High genetic variability in an RNA plant virus, banana mild mosaic virus |
title_full |
High genetic variability in an RNA plant virus, banana mild mosaic virus |
title_fullStr |
High genetic variability in an RNA plant virus, banana mild mosaic virus |
title_full_unstemmed |
High genetic variability in an RNA plant virus, banana mild mosaic virus |
title_sort |
high genetic variability in an rna plant virus, banana mild mosaic virus |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/527712/ http://agritrop.cirad.fr/527712/1/document_527712.pdf |
work_keys_str_mv |
AT teycheneypierreyves highgeneticvariabilityinanrnaplantvirusbananamildmosaicvirus AT laboureaunathalie highgeneticvariabilityinanrnaplantvirusbananamildmosaicvirus AT iskracaruanamarieline highgeneticvariabilityinanrnaplantvirusbananamildmosaicvirus AT candressethierry highgeneticvariabilityinanrnaplantvirusbananamildmosaicvirus |
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1792496250124238848 |