Characterisation of the effects of a major QTL of the partial resistance to Rice yellow mottle virus using a near-isogenic-line approach
Two quantitative trait loci (QTL12 and QTL7) acting in epistatis and associated with Rice yellow mottle virus (RYMV) infection were introgressed from the traditional Oryza sativa japonica cultivar Azucena into the improved indica cultivar IR64. The response to RYMV infection was followed over time in this nearly isogenic line (NIL). In leaves, the virus coat protein content was detected by ELISA and RNA by RT-PCR. In tissues, the coat protein was localised by immuno-fluorescence labelling and RNA by in situ hybridisation. We found (i) that Azucena combined a tolerance-characterised by mild symptoms despite generalised distribution and large accumulation of the virus-and a partial resistance, (ii) that partial resistance under the control of QTL12 and QTL7, dissociated in the NIL from tolerance and any other morphological or physiological Azucena traits, was expressed similarly in a japonica and in an indica genetic background, (iii) that partial resistance was transient and consisted in a 1-week delay in virus accumulation and symptom expression; passed this delay, partial resistance brokedown, and (iv) a similar delay in virus detection was observed at tissue levels, in the vascular bundle-sheath layers. Impaired virus movement caused by the absence or mutation of a QTL12-encoded plant factor necessary for virus transport through vascular bundle sheaths is proposed to explain this partial resistance.
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Subjects: | F30 - Génétique et amélioration des plantes, H20 - Maladies des plantes, Oryza, virus des végétaux, marqueur génétique, résistance aux maladies, http://aims.fao.org/aos/agrovoc/c_5435, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_2328, |
Online Access: | http://agritrop.cirad.fr/526817/ http://agritrop.cirad.fr/526817/1/526817.pdf |
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dig-cirad-fr-5268172024-01-28T13:39:32Z http://agritrop.cirad.fr/526817/ http://agritrop.cirad.fr/526817/ Characterisation of the effects of a major QTL of the partial resistance to Rice yellow mottle virus using a near-isogenic-line approach. Ioannidou D., Pinel A., Brugidou Christophe, Albar Laurence, Ahmadi Nourollah, Ghesquière Alain, Nicole Michel, Farguette Denis. 2003. Physiological and Molecular Plant Pathology, 63 (4) : 213-221.https://doi.org/10.1016/j.pmpp.2003.12.005 <https://doi.org/10.1016/j.pmpp.2003.12.005> Characterisation of the effects of a major QTL of the partial resistance to Rice yellow mottle virus using a near-isogenic-line approach Ioannidou, D. Pinel, A. Brugidou, Christophe Albar, Laurence Ahmadi, Nourollah Ghesquière, Alain Nicole, Michel Farguette, Denis eng 2003 Physiological and Molecular Plant Pathology F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza virus des végétaux marqueur génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_2328 Two quantitative trait loci (QTL12 and QTL7) acting in epistatis and associated with Rice yellow mottle virus (RYMV) infection were introgressed from the traditional Oryza sativa japonica cultivar Azucena into the improved indica cultivar IR64. The response to RYMV infection was followed over time in this nearly isogenic line (NIL). In leaves, the virus coat protein content was detected by ELISA and RNA by RT-PCR. In tissues, the coat protein was localised by immuno-fluorescence labelling and RNA by in situ hybridisation. We found (i) that Azucena combined a tolerance-characterised by mild symptoms despite generalised distribution and large accumulation of the virus-and a partial resistance, (ii) that partial resistance under the control of QTL12 and QTL7, dissociated in the NIL from tolerance and any other morphological or physiological Azucena traits, was expressed similarly in a japonica and in an indica genetic background, (iii) that partial resistance was transient and consisted in a 1-week delay in virus accumulation and symptom expression; passed this delay, partial resistance brokedown, and (iv) a similar delay in virus detection was observed at tissue levels, in the vascular bundle-sheath layers. Impaired virus movement caused by the absence or mutation of a QTL12-encoded plant factor necessary for virus transport through vascular bundle sheaths is proposed to explain this partial resistance. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/526817/1/526817.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.pmpp.2003.12.005 10.1016/j.pmpp.2003.12.005 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=186812 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pmpp.2003.12.005 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.pmpp.2003.12.005 |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza virus des végétaux marqueur génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_2328 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza virus des végétaux marqueur génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_2328 |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza virus des végétaux marqueur génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_2328 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza virus des végétaux marqueur génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_2328 Ioannidou, D. Pinel, A. Brugidou, Christophe Albar, Laurence Ahmadi, Nourollah Ghesquière, Alain Nicole, Michel Farguette, Denis Characterisation of the effects of a major QTL of the partial resistance to Rice yellow mottle virus using a near-isogenic-line approach |
description |
Two quantitative trait loci (QTL12 and QTL7) acting in epistatis and associated with Rice yellow mottle virus (RYMV) infection were introgressed from the traditional Oryza sativa japonica cultivar Azucena into the improved indica cultivar IR64. The response to RYMV infection was followed over time in this nearly isogenic line (NIL). In leaves, the virus coat protein content was detected by ELISA and RNA by RT-PCR. In tissues, the coat protein was localised by immuno-fluorescence labelling and RNA by in situ hybridisation. We found (i) that Azucena combined a tolerance-characterised by mild symptoms despite generalised distribution and large accumulation of the virus-and a partial resistance, (ii) that partial resistance under the control of QTL12 and QTL7, dissociated in the NIL from tolerance and any other morphological or physiological Azucena traits, was expressed similarly in a japonica and in an indica genetic background, (iii) that partial resistance was transient and consisted in a 1-week delay in virus accumulation and symptom expression; passed this delay, partial resistance brokedown, and (iv) a similar delay in virus detection was observed at tissue levels, in the vascular bundle-sheath layers. Impaired virus movement caused by the absence or mutation of a QTL12-encoded plant factor necessary for virus transport through vascular bundle sheaths is proposed to explain this partial resistance. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza virus des végétaux marqueur génétique résistance aux maladies http://aims.fao.org/aos/agrovoc/c_5435 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_2328 |
author |
Ioannidou, D. Pinel, A. Brugidou, Christophe Albar, Laurence Ahmadi, Nourollah Ghesquière, Alain Nicole, Michel Farguette, Denis |
author_facet |
Ioannidou, D. Pinel, A. Brugidou, Christophe Albar, Laurence Ahmadi, Nourollah Ghesquière, Alain Nicole, Michel Farguette, Denis |
author_sort |
Ioannidou, D. |
title |
Characterisation of the effects of a major QTL of the partial resistance to Rice yellow mottle virus using a near-isogenic-line approach |
title_short |
Characterisation of the effects of a major QTL of the partial resistance to Rice yellow mottle virus using a near-isogenic-line approach |
title_full |
Characterisation of the effects of a major QTL of the partial resistance to Rice yellow mottle virus using a near-isogenic-line approach |
title_fullStr |
Characterisation of the effects of a major QTL of the partial resistance to Rice yellow mottle virus using a near-isogenic-line approach |
title_full_unstemmed |
Characterisation of the effects of a major QTL of the partial resistance to Rice yellow mottle virus using a near-isogenic-line approach |
title_sort |
characterisation of the effects of a major qtl of the partial resistance to rice yellow mottle virus using a near-isogenic-line approach |
url |
http://agritrop.cirad.fr/526817/ http://agritrop.cirad.fr/526817/1/526817.pdf |
work_keys_str_mv |
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