Enzymatic degradation of cell wall polysaccharides from mango (Mangifera indica L.) puree
Ripe mango puree (Smith cultivar) was treated with fungal polysaccharidases containing pectinolytic, hemicellulolytic, and cellulolytic activities for 2 h at 50°C. A loss of 30% of the cell wall material (CWM) was measured. CWM polysaccharides were hydrolyzed to varying degrees: 88, 65, and 65% of, respectively, galacturonic acid-, arabinose-, and rhamnose-containing polymers were hydrolyzed, whereas 50% of cellulose was degraded. After 30 min of treatment, the ethanol precipitation test on the serum was negative, indicating that pectic substances were rapidly hydrolyzed. Oligogalacturonic acids (degree of polymerization, 1-12) were observed in the serum. A viscosity drop of 90% was measured after 2 h, confirming the dominant role of pectic substances in puree viscosity.
Main Authors: | , , , |
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Format: | article biblioteca |
Language: | eng |
Subjects: | Q02 - Traitement et conservation des produits alimentaires, Mangifera indica, mangue, dégradation, traitement, hydrolyse enzymatique, polyholoside, membrane cellulaire, Viscosité, pulpe de fruits, purée de fruit, http://aims.fao.org/aos/agrovoc/c_4575, http://aims.fao.org/aos/agrovoc/c_12367, http://aims.fao.org/aos/agrovoc/c_2159, http://aims.fao.org/aos/agrovoc/c_6195, http://aims.fao.org/aos/agrovoc/c_27512, http://aims.fao.org/aos/agrovoc/c_6097, http://aims.fao.org/aos/agrovoc/c_9693, http://aims.fao.org/aos/agrovoc/c_15219, http://aims.fao.org/aos/agrovoc/c_3126, http://aims.fao.org/aos/agrovoc/c_11030, |
Online Access: | http://agritrop.cirad.fr/523349/ http://agritrop.cirad.fr/523349/1/523349.pdf |
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Summary: | Ripe mango puree (Smith cultivar) was treated with fungal polysaccharidases containing pectinolytic, hemicellulolytic, and cellulolytic activities for 2 h at 50°C. A loss of 30% of the cell wall material (CWM) was measured. CWM polysaccharides were hydrolyzed to varying degrees: 88, 65, and 65% of, respectively, galacturonic acid-, arabinose-, and rhamnose-containing polymers were hydrolyzed, whereas 50% of cellulose was degraded. After 30 min of treatment, the ethanol precipitation test on the serum was negative, indicating that pectic substances were rapidly hydrolyzed. Oligogalacturonic acids (degree of polymerization, 1-12) were observed in the serum. A viscosity drop of 90% was measured after 2 h, confirming the dominant role of pectic substances in puree viscosity. |
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