Contribution to the development of a biological control method against crown rot disease
Crown rot is an important banana post-harvest disease infecting the pad of cut hands. The disease spreads rapidly during fruit ripening and contaminates the pedicel and ultimately the pulp. The disease is caused by a complex of fungi (occasionally bacteria) with one main pathogen: Colletotrichum musae. The aim of this study was to evaluate the antagonistic activity of two yeast strains against the parasitic complex in relation with their concentrations and their incubation period. The antagonistic activity of Pichia anomala strain K and Candida oleophila strain O, applied at three concentrations (106, 107 , 108 cfu/ml), have been evaluated on Colletotrichum musae (103 conidia/ml), Fusarium moniliforme (104 conidia/ml), Cephalosporium sp. (104 conidia/ml) or on a parasitic complex formed by the association of these three fungi. One hundred pl of the pathogen(s) were deposited on the crown of 4 'Grande naine' (AAA) hands. After 15 minutes, the crowns were soaked in a suspension of strain K or strain O for 10 seconds. Before the evaluation of the pathogen(s) progression into the crown, bananas were incubated for 13 days under conditions similar to exportation. Antagonistic effects have been observed on C. musae, F. moniliforme and the complex. Among the various treatments, the application of strain O at 108 cfu/ml showed the highest protective level (56%). This level was higher than those observed against the fungi separately inoculated. The influence of incubation period between strain O (108 ufc/ml) and complex inoculation has also been studied. Strain O was applied 24h before the complex, but also 15' or 3h after its inoculation. The highest protective level was observed when strain O was applied 24h before the complex. Finally, a correlation between symptom severity and protective level of strain O has been highlighted. When the severity of the disease increased, protection by the yeast decreased. In conclusion, both strains have showed antagonistic effects but strain O was more effective than strain K. (Texte intégral)
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Subjects: | H10 - Ravageurs des plantes, H20 - Maladies des plantes, Musa, ravageur des plantes, Colletotrichum, contrôle de maladies, lutte biologique, parasite, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_16196, http://aims.fao.org/aos/agrovoc/c_1761, http://aims.fao.org/aos/agrovoc/c_2327, http://aims.fao.org/aos/agrovoc/c_918, http://aims.fao.org/aos/agrovoc/c_5574, |
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dig-cirad-fr-5221562024-01-28T12:47:26Z http://agritrop.cirad.fr/522156/ http://agritrop.cirad.fr/522156/ Contribution to the development of a biological control method against crown rot disease. Lassois Ludivine, De Lapeyre de Bellaire Luc, Busogoro J.P., Jijakli M. Haïssam. 2004. In : First International congress on Musa: harnessing research for improved livelihoods, 6-9 July 2004, Penang, Malaysia. Abstract guide. Picq Claudine (ed.), Vézina Anne (ed.). INIBAP, MARDI, BAPNET, University of Malaya, University of Putra, IPGRI. Montpellier : INIBAP, Résumé, 163. International Congress on Musa: Harnessing Research for Improved Livelihoods. 1, Penang, Malaisie, 6 Juillet 2004/9 Juillet 2004. Contribution to the development of a biological control method against crown rot disease Lassois, Ludivine De Lapeyre de Bellaire, Luc Busogoro, J.P. Jijakli, M. Haïssam eng 2004 INIBAP First International congress on Musa: harnessing research for improved livelihoods, 6-9 July 2004, Penang, Malaysia. Abstract guide H10 - Ravageurs des plantes H20 - Maladies des plantes Musa ravageur des plantes Colletotrichum contrôle de maladies lutte biologique parasite http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_5574 Crown rot is an important banana post-harvest disease infecting the pad of cut hands. The disease spreads rapidly during fruit ripening and contaminates the pedicel and ultimately the pulp. The disease is caused by a complex of fungi (occasionally bacteria) with one main pathogen: Colletotrichum musae. The aim of this study was to evaluate the antagonistic activity of two yeast strains against the parasitic complex in relation with their concentrations and their incubation period. The antagonistic activity of Pichia anomala strain K and Candida oleophila strain O, applied at three concentrations (106, 107 , 108 cfu/ml), have been evaluated on Colletotrichum musae (103 conidia/ml), Fusarium moniliforme (104 conidia/ml), Cephalosporium sp. (104 conidia/ml) or on a parasitic complex formed by the association of these three fungi. One hundred pl of the pathogen(s) were deposited on the crown of 4 'Grande naine' (AAA) hands. After 15 minutes, the crowns were soaked in a suspension of strain K or strain O for 10 seconds. Before the evaluation of the pathogen(s) progression into the crown, bananas were incubated for 13 days under conditions similar to exportation. Antagonistic effects have been observed on C. musae, F. moniliforme and the complex. Among the various treatments, the application of strain O at 108 cfu/ml showed the highest protective level (56%). This level was higher than those observed against the fungi separately inoculated. The influence of incubation period between strain O (108 ufc/ml) and complex inoculation has also been studied. Strain O was applied 24h before the complex, but also 15' or 3h after its inoculation. The highest protective level was observed when strain O was applied 24h before the complex. Finally, a correlation between symptom severity and protective level of strain O has been highlighted. When the severity of the disease increased, protection by the yeast decreased. In conclusion, both strains have showed antagonistic effects but strain O was more effective than strain K. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=182712 |
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H10 - Ravageurs des plantes H20 - Maladies des plantes Musa ravageur des plantes Colletotrichum contrôle de maladies lutte biologique parasite http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_5574 H10 - Ravageurs des plantes H20 - Maladies des plantes Musa ravageur des plantes Colletotrichum contrôle de maladies lutte biologique parasite http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_5574 |
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H10 - Ravageurs des plantes H20 - Maladies des plantes Musa ravageur des plantes Colletotrichum contrôle de maladies lutte biologique parasite http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_5574 H10 - Ravageurs des plantes H20 - Maladies des plantes Musa ravageur des plantes Colletotrichum contrôle de maladies lutte biologique parasite http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_5574 Lassois, Ludivine De Lapeyre de Bellaire, Luc Busogoro, J.P. Jijakli, M. Haïssam Contribution to the development of a biological control method against crown rot disease |
description |
Crown rot is an important banana post-harvest disease infecting the pad of cut hands. The disease spreads rapidly during fruit ripening and contaminates the pedicel and ultimately the pulp. The disease is caused by a complex of fungi (occasionally bacteria) with one main pathogen: Colletotrichum musae. The aim of this study was to evaluate the antagonistic activity of two yeast strains against the parasitic complex in relation with their concentrations and their incubation period. The antagonistic activity of Pichia anomala strain K and Candida oleophila strain O, applied at three concentrations (106, 107 , 108 cfu/ml), have been evaluated on Colletotrichum musae (103 conidia/ml), Fusarium moniliforme (104 conidia/ml), Cephalosporium sp. (104 conidia/ml) or on a parasitic complex formed by the association of these three fungi. One hundred pl of the pathogen(s) were deposited on the crown of 4 'Grande naine' (AAA) hands. After 15 minutes, the crowns were soaked in a suspension of strain K or strain O for 10 seconds. Before the evaluation of the pathogen(s) progression into the crown, bananas were incubated for 13 days under conditions similar to exportation. Antagonistic effects have been observed on C. musae, F. moniliforme and the complex. Among the various treatments, the application of strain O at 108 cfu/ml showed the highest protective level (56%). This level was higher than those observed against the fungi separately inoculated. The influence of incubation period between strain O (108 ufc/ml) and complex inoculation has also been studied. Strain O was applied 24h before the complex, but also 15' or 3h after its inoculation. The highest protective level was observed when strain O was applied 24h before the complex. Finally, a correlation between symptom severity and protective level of strain O has been highlighted. When the severity of the disease increased, protection by the yeast decreased. In conclusion, both strains have showed antagonistic effects but strain O was more effective than strain K. (Texte intégral) |
format |
conference_item |
topic_facet |
H10 - Ravageurs des plantes H20 - Maladies des plantes Musa ravageur des plantes Colletotrichum contrôle de maladies lutte biologique parasite http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_1761 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_918 http://aims.fao.org/aos/agrovoc/c_5574 |
author |
Lassois, Ludivine De Lapeyre de Bellaire, Luc Busogoro, J.P. Jijakli, M. Haïssam |
author_facet |
Lassois, Ludivine De Lapeyre de Bellaire, Luc Busogoro, J.P. Jijakli, M. Haïssam |
author_sort |
Lassois, Ludivine |
title |
Contribution to the development of a biological control method against crown rot disease |
title_short |
Contribution to the development of a biological control method against crown rot disease |
title_full |
Contribution to the development of a biological control method against crown rot disease |
title_fullStr |
Contribution to the development of a biological control method against crown rot disease |
title_full_unstemmed |
Contribution to the development of a biological control method against crown rot disease |
title_sort |
contribution to the development of a biological control method against crown rot disease |
publisher |
INIBAP |
url |
http://agritrop.cirad.fr/522156/ |
work_keys_str_mv |
AT lassoisludivine contributiontothedevelopmentofabiologicalcontrolmethodagainstcrownrotdisease AT delapeyredebellaireluc contributiontothedevelopmentofabiologicalcontrolmethodagainstcrownrotdisease AT busogorojp contributiontothedevelopmentofabiologicalcontrolmethodagainstcrownrotdisease AT jijaklimhaissam contributiontothedevelopmentofabiologicalcontrolmethodagainstcrownrotdisease |
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