Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in africa and Europe
Bluetongue (BT) and African Horse Sickness (AHS) are infectious arthropod-borne viral diseases affecting ruminants and horses, respectively. Culicoides imicola Kieffer, 1913, a biting midge, is the principal vector of these livestock diseases in Africa and Europe. Recently bluetongue disease has re-emerged in the Mediterranean Basin and has had a devastating effect on the sheep industry in Italy and on the islands of Sicily, Sardinia, Corsica and the Balearics, but fortunately, has not penetrated onto mainland France and Spain. To survey for the presence of C. imicola, an extensive light-trap network for the collection of Culicoides, was implemented in 2002 in southern mainland France. The morphological identification of Culicoides can be both tedious and time-consuming because its size ranges from 1.5 to 3 mm. Therefore, an ITS 1 rDNA polymerase chain reaction (PCR)-based diagnostic assay was developed to rapidly and reliably identify Culicoides spp. and C. imicola. The aim of this work was to set up a rapid test for the detection of C. imicola amongst a pool of insects collected in areas at risk for BT. The sequence similarity of the rDNA (nuclear ribosomal DNA), which is greater within species than between species, is the foundation of its utilisation in species-diagnostic assays. The alignment of the 11 ITS 1 sequences of Culicoides obtained from Genbank and EMBL databases helped us to identify one region in the 5' end and one in the 3' end that appear highly conserved. PCR primers were designed within these regions to amplify genus-specific fragments. In order to set up a C. imicola-specific PCR, another forward primer was designed and used in combination with the previously designed reverse primer. These primers proved to be highly specific and sensitive and permitted a rapid diagnostic separation of C. imicola from Culicoides spp.
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Language: | eng |
Subjects: | L73 - Maladies des animaux, contrôle de maladies, biologie moléculaire, fièvre catarrhale ovine, Culicoides, PCR, http://aims.fao.org/aos/agrovoc/c_2327, http://aims.fao.org/aos/agrovoc/c_4891, http://aims.fao.org/aos/agrovoc/c_976, http://aims.fao.org/aos/agrovoc/c_10196, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_2724, http://aims.fao.org/aos/agrovoc/c_165, |
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dig-cirad-fr-5204582024-01-28T12:32:50Z http://agritrop.cirad.fr/520458/ http://agritrop.cirad.fr/520458/ Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in africa and Europe. Cetre-Sossah Catherine, Baldet Thierry, Delecolle Jean Claude, Mathieu Bruno, Perrin Aurélie, Grillet Colette, Albina Emmanuel. 2004. Veterinary Research, 35 : 325-337.https://doi.org/10.1051/vetres:2004015 <https://doi.org/10.1051/vetres:2004015> Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in africa and Europe Cetre-Sossah, Catherine Baldet, Thierry Delecolle, Jean Claude Mathieu, Bruno Perrin, Aurélie Grillet, Colette Albina, Emmanuel eng 2004 Veterinary Research L73 - Maladies des animaux contrôle de maladies biologie moléculaire fièvre catarrhale ovine Culicoides PCR http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_976 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_34079 Europe Afrique http://aims.fao.org/aos/agrovoc/c_2724 http://aims.fao.org/aos/agrovoc/c_165 Bluetongue (BT) and African Horse Sickness (AHS) are infectious arthropod-borne viral diseases affecting ruminants and horses, respectively. Culicoides imicola Kieffer, 1913, a biting midge, is the principal vector of these livestock diseases in Africa and Europe. Recently bluetongue disease has re-emerged in the Mediterranean Basin and has had a devastating effect on the sheep industry in Italy and on the islands of Sicily, Sardinia, Corsica and the Balearics, but fortunately, has not penetrated onto mainland France and Spain. To survey for the presence of C. imicola, an extensive light-trap network for the collection of Culicoides, was implemented in 2002 in southern mainland France. The morphological identification of Culicoides can be both tedious and time-consuming because its size ranges from 1.5 to 3 mm. Therefore, an ITS 1 rDNA polymerase chain reaction (PCR)-based diagnostic assay was developed to rapidly and reliably identify Culicoides spp. and C. imicola. The aim of this work was to set up a rapid test for the detection of C. imicola amongst a pool of insects collected in areas at risk for BT. The sequence similarity of the rDNA (nuclear ribosomal DNA), which is greater within species than between species, is the foundation of its utilisation in species-diagnostic assays. The alignment of the 11 ITS 1 sequences of Culicoides obtained from Genbank and EMBL databases helped us to identify one region in the 5' end and one in the 3' end that appear highly conserved. PCR primers were designed within these regions to amplify genus-specific fragments. In order to set up a C. imicola-specific PCR, another forward primer was designed and used in combination with the previously designed reverse primer. These primers proved to be highly specific and sensitive and permitted a rapid diagnostic separation of C. imicola from Culicoides spp. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/520458/1/520458.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1051/vetres:2004015 10.1051/vetres:2004015 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=181748 info:eu-repo/semantics/altIdentifier/doi/10.1051/vetres:2004015 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1051/vetres:2004015 |
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L73 - Maladies des animaux contrôle de maladies biologie moléculaire fièvre catarrhale ovine Culicoides PCR http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_976 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2724 http://aims.fao.org/aos/agrovoc/c_165 L73 - Maladies des animaux contrôle de maladies biologie moléculaire fièvre catarrhale ovine Culicoides PCR http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_976 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2724 http://aims.fao.org/aos/agrovoc/c_165 |
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L73 - Maladies des animaux contrôle de maladies biologie moléculaire fièvre catarrhale ovine Culicoides PCR http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_976 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2724 http://aims.fao.org/aos/agrovoc/c_165 L73 - Maladies des animaux contrôle de maladies biologie moléculaire fièvre catarrhale ovine Culicoides PCR http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_976 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2724 http://aims.fao.org/aos/agrovoc/c_165 Cetre-Sossah, Catherine Baldet, Thierry Delecolle, Jean Claude Mathieu, Bruno Perrin, Aurélie Grillet, Colette Albina, Emmanuel Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in africa and Europe |
description |
Bluetongue (BT) and African Horse Sickness (AHS) are infectious arthropod-borne viral diseases affecting ruminants and horses, respectively. Culicoides imicola Kieffer, 1913, a biting midge, is the principal vector of these livestock diseases in Africa and Europe. Recently bluetongue disease has re-emerged in the Mediterranean Basin and has had a devastating effect on the sheep industry in Italy and on the islands of Sicily, Sardinia, Corsica and the Balearics, but fortunately, has not penetrated onto mainland France and Spain. To survey for the presence of C. imicola, an extensive light-trap network for the collection of Culicoides, was implemented in 2002 in southern mainland France. The morphological identification of Culicoides can be both tedious and time-consuming because its size ranges from 1.5 to 3 mm. Therefore, an ITS 1 rDNA polymerase chain reaction (PCR)-based diagnostic assay was developed to rapidly and reliably identify Culicoides spp. and C. imicola. The aim of this work was to set up a rapid test for the detection of C. imicola amongst a pool of insects collected in areas at risk for BT. The sequence similarity of the rDNA (nuclear ribosomal DNA), which is greater within species than between species, is the foundation of its utilisation in species-diagnostic assays. The alignment of the 11 ITS 1 sequences of Culicoides obtained from Genbank and EMBL databases helped us to identify one region in the 5' end and one in the 3' end that appear highly conserved. PCR primers were designed within these regions to amplify genus-specific fragments. In order to set up a C. imicola-specific PCR, another forward primer was designed and used in combination with the previously designed reverse primer. These primers proved to be highly specific and sensitive and permitted a rapid diagnostic separation of C. imicola from Culicoides spp. |
format |
article |
topic_facet |
L73 - Maladies des animaux contrôle de maladies biologie moléculaire fièvre catarrhale ovine Culicoides PCR http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_976 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2724 http://aims.fao.org/aos/agrovoc/c_165 |
author |
Cetre-Sossah, Catherine Baldet, Thierry Delecolle, Jean Claude Mathieu, Bruno Perrin, Aurélie Grillet, Colette Albina, Emmanuel |
author_facet |
Cetre-Sossah, Catherine Baldet, Thierry Delecolle, Jean Claude Mathieu, Bruno Perrin, Aurélie Grillet, Colette Albina, Emmanuel |
author_sort |
Cetre-Sossah, Catherine |
title |
Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in africa and Europe |
title_short |
Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in africa and Europe |
title_full |
Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in africa and Europe |
title_fullStr |
Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in africa and Europe |
title_full_unstemmed |
Molecular detection of Culicoides spp. and Culicoides imicola, the principal vector of bluetongue (BT) and African horse sickness (AHS) in africa and Europe |
title_sort |
molecular detection of culicoides spp. and culicoides imicola, the principal vector of bluetongue (bt) and african horse sickness (ahs) in africa and europe |
url |
http://agritrop.cirad.fr/520458/ http://agritrop.cirad.fr/520458/1/520458.pdf |
work_keys_str_mv |
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