Genome distribution and characterization of EST derived sugarcane resistance gene analogs
A large sugarcane EST project recently gave us access to 261,609 sugarcane EST sequences assembled into 81,223 clusters. Among them, we identified 88 resistance gene analogs (RGAs) based on their homology with typical pathogen resistance genes using a stringent BLAST search with an expect value of e-50. They included representatives of the three groups of resistance genes with NBS/LRR, LRR or S/T KINASE domains. Fifty RGAs revealed a total of 148 single dose polymorphic RFLP markers that could be located on the sugarcane reference genetic map (cultivar R570, 2n=ca 115). Fifty-five SSR loci corresponding to 134 markers in R570 were also mapped to enable the classification of the various haplotypes into homology groups. Several RGA clusters were found. One cluster of two LRR-like loci mapped close to the only disease resistance gene known so far in sugarcane, that confers resistance to common rust. Detailed sequence comparison between two NBS/LRR RGA clusters in relation to their orthologs in rice and maize suggests their polyphyletic origins and indicates that the divergence between sugarcane paralogous RGAs can be larger than the divergence with an orthologue in a distant species. (Texte intégral)
Main Authors: | , , , , , , , |
---|---|
Format: | conference_item biblioteca |
Language: | eng |
Published: |
CIRAD-CA
|
Subjects: | U30 - Méthodes de recherche, F30 - Génétique et amélioration des plantes, Saccharum, séquence nucléotidique, sonde nucléique, identification, gène, résistance aux maladies, carte génétique, http://aims.fao.org/aos/agrovoc/c_6725, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_35729, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_24002, |
Online Access: | http://agritrop.cirad.fr/519858/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-519858 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5198582024-01-28T12:26:30Z http://agritrop.cirad.fr/519858/ http://agritrop.cirad.fr/519858/ Genome distribution and characterization of EST derived sugarcane resistance gene analogs. Rossi Magdalena, Araujo Paula G., Paulet Florence, Garsmeur Olivier, Dias Viviane M., Chen Hui, Van Sluys Marie-Anne, D'Hont Angélique. 2003. In : IVth Molecular biology workshop, Montpellier, France, 7-11 April 2003 [Abstracts]. ISSCT, Languedoc-Roussillon-Conseil régional, CIRAD-CA. Montpellier : CIRAD-CA, Résumé, 9. Molecular Biology Workshop. 4, Montpellier, France, 7 Avril 2003/11 Avril 2003. Genome distribution and characterization of EST derived sugarcane resistance gene analogs Rossi, Magdalena Araujo, Paula G. Paulet, Florence Garsmeur, Olivier Dias, Viviane M. Chen Hui, Van Sluys, Marie-Anne D'Hont, Angélique eng 2003 CIRAD-CA IVth Molecular biology workshop, Montpellier, France, 7-11 April 2003 [Abstracts] U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique sonde nucléique identification gène résistance aux maladies carte génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35729 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24002 A large sugarcane EST project recently gave us access to 261,609 sugarcane EST sequences assembled into 81,223 clusters. Among them, we identified 88 resistance gene analogs (RGAs) based on their homology with typical pathogen resistance genes using a stringent BLAST search with an expect value of e-50. They included representatives of the three groups of resistance genes with NBS/LRR, LRR or S/T KINASE domains. Fifty RGAs revealed a total of 148 single dose polymorphic RFLP markers that could be located on the sugarcane reference genetic map (cultivar R570, 2n=ca 115). Fifty-five SSR loci corresponding to 134 markers in R570 were also mapped to enable the classification of the various haplotypes into homology groups. Several RGA clusters were found. One cluster of two LRR-like loci mapped close to the only disease resistance gene known so far in sugarcane, that confers resistance to common rust. Detailed sequence comparison between two NBS/LRR RGA clusters in relation to their orthologs in rice and maize suggests their polyphyletic origins and indicates that the divergence between sugarcane paralogous RGAs can be larger than the divergence with an orthologue in a distant species. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://agritrop.cirad.fr/519847/ |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique sonde nucléique identification gène résistance aux maladies carte génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35729 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24002 U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique sonde nucléique identification gène résistance aux maladies carte génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35729 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24002 |
spellingShingle |
U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique sonde nucléique identification gène résistance aux maladies carte génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35729 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24002 U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique sonde nucléique identification gène résistance aux maladies carte génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35729 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24002 Rossi, Magdalena Araujo, Paula G. Paulet, Florence Garsmeur, Olivier Dias, Viviane M. Chen Hui, Van Sluys, Marie-Anne D'Hont, Angélique Genome distribution and characterization of EST derived sugarcane resistance gene analogs |
description |
A large sugarcane EST project recently gave us access to 261,609 sugarcane EST sequences assembled into 81,223 clusters. Among them, we identified 88 resistance gene analogs (RGAs) based on their homology with typical pathogen resistance genes using a stringent BLAST search with an expect value of e-50. They included representatives of the three groups of resistance genes with NBS/LRR, LRR or S/T KINASE domains. Fifty RGAs revealed a total of 148 single dose polymorphic RFLP markers that could be located on the sugarcane reference genetic map (cultivar R570, 2n=ca 115). Fifty-five SSR loci corresponding to 134 markers in R570 were also mapped to enable the classification of the various haplotypes into homology groups. Several RGA clusters were found. One cluster of two LRR-like loci mapped close to the only disease resistance gene known so far in sugarcane, that confers resistance to common rust. Detailed sequence comparison between two NBS/LRR RGA clusters in relation to their orthologs in rice and maize suggests their polyphyletic origins and indicates that the divergence between sugarcane paralogous RGAs can be larger than the divergence with an orthologue in a distant species. (Texte intégral) |
format |
conference_item |
topic_facet |
U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique sonde nucléique identification gène résistance aux maladies carte génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_35729 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24002 |
author |
Rossi, Magdalena Araujo, Paula G. Paulet, Florence Garsmeur, Olivier Dias, Viviane M. Chen Hui, Van Sluys, Marie-Anne D'Hont, Angélique |
author_facet |
Rossi, Magdalena Araujo, Paula G. Paulet, Florence Garsmeur, Olivier Dias, Viviane M. Chen Hui, Van Sluys, Marie-Anne D'Hont, Angélique |
author_sort |
Rossi, Magdalena |
title |
Genome distribution and characterization of EST derived sugarcane resistance gene analogs |
title_short |
Genome distribution and characterization of EST derived sugarcane resistance gene analogs |
title_full |
Genome distribution and characterization of EST derived sugarcane resistance gene analogs |
title_fullStr |
Genome distribution and characterization of EST derived sugarcane resistance gene analogs |
title_full_unstemmed |
Genome distribution and characterization of EST derived sugarcane resistance gene analogs |
title_sort |
genome distribution and characterization of est derived sugarcane resistance gene analogs |
publisher |
CIRAD-CA |
url |
http://agritrop.cirad.fr/519858/ |
work_keys_str_mv |
AT rossimagdalena genomedistributionandcharacterizationofestderivedsugarcaneresistancegeneanalogs AT araujopaulag genomedistributionandcharacterizationofestderivedsugarcaneresistancegeneanalogs AT pauletflorence genomedistributionandcharacterizationofestderivedsugarcaneresistancegeneanalogs AT garsmeurolivier genomedistributionandcharacterizationofestderivedsugarcaneresistancegeneanalogs AT diasvivianem genomedistributionandcharacterizationofestderivedsugarcaneresistancegeneanalogs AT chenhui genomedistributionandcharacterizationofestderivedsugarcaneresistancegeneanalogs AT vansluysmarieanne genomedistributionandcharacterizationofestderivedsugarcaneresistancegeneanalogs AT dhontangelique genomedistributionandcharacterizationofestderivedsugarcaneresistancegeneanalogs |
_version_ |
1792495843820961792 |