Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes
Xanthomonas albilineans, which causes leaf scald disease of sugarcane, produces a highly potent pathotoxin called albicidin. We report here sequencing and homology analysis of the major gene cluster, XALB1 (55,839 bp), and a second, smaller region, XALB2 (2,986 bp), involved in albicidin biosynthesis. XALB1 contains 20 open reading frames, including i) three large genes with a modular architecture characteristic of polyketide synthases (PKSs) and nonribosomal peptide synthases (NRPSs) and ii) several putative modifying, regulatory, and resistance genes. Sequencing and complementation studies of six albicidin-defective mutants enabled us to confirm the involvement of the three PKS and NRPS genes encoded by XALB1 in albicidin production. XALB2 contains only one gene that is required for post-translational activation of PKS and NRPS enzymes, confirming the involvement of these enzymes in albicidin biosynthesis. In silico analysis of these three PKS or NRPS enzymes allowed us to propose a model for the albicidin backbone assembly and to gain insight into the structural features of this pathotoxin. This is the first description of a complete mixed PKS-NRPS gene cluster for toxin production in the genus Xanthomonas.
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dig-cirad-fr-5190672022-04-21T16:28:21Z http://agritrop.cirad.fr/519067/ http://agritrop.cirad.fr/519067/ Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes. Royer Monique, Costet Laurent, Vivien Eric, Bes Martine, Cousin Arnaud, Damais Anne, Pieretti Isabelle, Savin Alexandre, Megessier Sandrine, Viard Magali, Frutos Roger, Gabriel Dean W., Rott Philippe. 2004. Molecular Plant-Microbe Interactions, 17 (4) : 414-427.https://doi.org/10.1094/MPMI.2004.17.4.414 <https://doi.org/10.1094/MPMI.2004.17.4.414> Researchers Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes Royer, Monique Costet, Laurent Vivien, Eric Bes, Martine Cousin, Arnaud Damais, Anne Pieretti, Isabelle Savin, Alexandre Megessier, Sandrine Viard, Magali Frutos, Roger Gabriel, Dean W. Rott, Philippe eng 2004 Molecular Plant-Microbe Interactions H20 - Maladies des plantes Xanthomonas albilineans, which causes leaf scald disease of sugarcane, produces a highly potent pathotoxin called albicidin. We report here sequencing and homology analysis of the major gene cluster, XALB1 (55,839 bp), and a second, smaller region, XALB2 (2,986 bp), involved in albicidin biosynthesis. XALB1 contains 20 open reading frames, including i) three large genes with a modular architecture characteristic of polyketide synthases (PKSs) and nonribosomal peptide synthases (NRPSs) and ii) several putative modifying, regulatory, and resistance genes. Sequencing and complementation studies of six albicidin-defective mutants enabled us to confirm the involvement of the three PKS and NRPS genes encoded by XALB1 in albicidin production. XALB2 contains only one gene that is required for post-translational activation of PKS and NRPS enzymes, confirming the involvement of these enzymes in albicidin biosynthesis. In silico analysis of these three PKS or NRPS enzymes allowed us to propose a model for the albicidin backbone assembly and to gain insight into the structural features of this pathotoxin. This is the first description of a complete mixed PKS-NRPS gene cluster for toxin production in the genus Xanthomonas. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/519067/1/document_519067.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1094/MPMI.2004.17.4.414 10.1094/MPMI.2004.17.4.414 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=180349 info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI.2004.17.4.414 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1094/MPMI.2004.17.4.414 |
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H20 - Maladies des plantes H20 - Maladies des plantes Royer, Monique Costet, Laurent Vivien, Eric Bes, Martine Cousin, Arnaud Damais, Anne Pieretti, Isabelle Savin, Alexandre Megessier, Sandrine Viard, Magali Frutos, Roger Gabriel, Dean W. Rott, Philippe Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes |
description |
Xanthomonas albilineans, which causes leaf scald disease of sugarcane, produces a highly potent pathotoxin called albicidin. We report here sequencing and homology analysis of the major gene cluster, XALB1 (55,839 bp), and a second, smaller region, XALB2 (2,986 bp), involved in albicidin biosynthesis. XALB1 contains 20 open reading frames, including i) three large genes with a modular architecture characteristic of polyketide synthases (PKSs) and nonribosomal peptide synthases (NRPSs) and ii) several putative modifying, regulatory, and resistance genes. Sequencing and complementation studies of six albicidin-defective mutants enabled us to confirm the involvement of the three PKS and NRPS genes encoded by XALB1 in albicidin production. XALB2 contains only one gene that is required for post-translational activation of PKS and NRPS enzymes, confirming the involvement of these enzymes in albicidin biosynthesis. In silico analysis of these three PKS or NRPS enzymes allowed us to propose a model for the albicidin backbone assembly and to gain insight into the structural features of this pathotoxin. This is the first description of a complete mixed PKS-NRPS gene cluster for toxin production in the genus Xanthomonas. |
format |
article |
topic_facet |
H20 - Maladies des plantes |
author |
Royer, Monique Costet, Laurent Vivien, Eric Bes, Martine Cousin, Arnaud Damais, Anne Pieretti, Isabelle Savin, Alexandre Megessier, Sandrine Viard, Magali Frutos, Roger Gabriel, Dean W. Rott, Philippe |
author_facet |
Royer, Monique Costet, Laurent Vivien, Eric Bes, Martine Cousin, Arnaud Damais, Anne Pieretti, Isabelle Savin, Alexandre Megessier, Sandrine Viard, Magali Frutos, Roger Gabriel, Dean W. Rott, Philippe |
author_sort |
Royer, Monique |
title |
Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes |
title_short |
Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes |
title_full |
Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes |
title_fullStr |
Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes |
title_full_unstemmed |
Albicidin pathotoxin produced by Xanthomonas albilineans is encoded by three large PKS and NRPS genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes |
title_sort |
albicidin pathotoxin produced by xanthomonas albilineans is encoded by three large pks and nrps genes present in a gene cluster also containing several putative modifying, regulatory, and resistance genes |
url |
http://agritrop.cirad.fr/519067/ http://agritrop.cirad.fr/519067/1/document_519067.pdf |
work_keys_str_mv |
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