A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats
Mycoplasma putrefaciens is listed as one of the etiologic agents of the contagious agalactia syndrome by the world organisation for animal health. This species has been characterized only recently, 1974, and the number of outbreaks caused by this microorganism so far is very scarce. It induces mastitis in infected goats although other symptoms such as arthritis in adults and septicaemia in kids are also frequently described. Up to now, the identification of M. putrefaciens relied on classical isolation and identification techniques which present a number of limitations. Specific primers for PCR have been designed based on sequence comparisons of the Arc B gene among the 'Mycoplasma mycoides cluster' and related species such as Mycoplasma cottewii and Mycoplasma yeatsii. Sequence alignments confirmed the taxonomic position of M. putrefaciens, which is related to the W. mycoides cluster' but also very close to M. yeatsii. The polymorphism observed amongst the different Arc B sequences allowed the determination of a primer pair yielding a specific amplification of a 316 bp-long DNA fragment by PCR. This PCR was validated in two different laboratories with a variety of mycoplasma strains isolated from goats. This new PCR technique will be very useful for a quicker determination of M. putrefaciens strains as well as a better understanding of the prevalence of M. putrefaciens infections.
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Subjects: | L74 - Troubles divers des animaux, chèvre, PCR, mycoplasmose, Mycoplasma putrefaciens, http://aims.fao.org/aos/agrovoc/c_5066, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_5022, http://aims.fao.org/aos/agrovoc/c_37783, |
Online Access: | http://agritrop.cirad.fr/517194/ http://agritrop.cirad.fr/517194/1/517194.pdf |
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dig-cirad-fr-5171942024-01-28T11:59:21Z http://agritrop.cirad.fr/517194/ http://agritrop.cirad.fr/517194/ A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats. Peyraud Armelle, Woubit Salah, Poveda J.B., De La Fé C., Mercier Pascale, Thiaucourt François. 2003. Molecular and Cellular Probes, 17 : 289-294.https://doi.org/10.1016/j.mcp.2003.07.006 <https://doi.org/10.1016/j.mcp.2003.07.006> A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats Peyraud, Armelle Woubit, Salah Poveda, J.B. De La Fé, C. Mercier, Pascale Thiaucourt, François eng 2003 Molecular and Cellular Probes L74 - Troubles divers des animaux chèvre PCR mycoplasmose Mycoplasma putrefaciens http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_37783 Mycoplasma putrefaciens is listed as one of the etiologic agents of the contagious agalactia syndrome by the world organisation for animal health. This species has been characterized only recently, 1974, and the number of outbreaks caused by this microorganism so far is very scarce. It induces mastitis in infected goats although other symptoms such as arthritis in adults and septicaemia in kids are also frequently described. Up to now, the identification of M. putrefaciens relied on classical isolation and identification techniques which present a number of limitations. Specific primers for PCR have been designed based on sequence comparisons of the Arc B gene among the 'Mycoplasma mycoides cluster' and related species such as Mycoplasma cottewii and Mycoplasma yeatsii. Sequence alignments confirmed the taxonomic position of M. putrefaciens, which is related to the W. mycoides cluster' but also very close to M. yeatsii. The polymorphism observed amongst the different Arc B sequences allowed the determination of a primer pair yielding a specific amplification of a 316 bp-long DNA fragment by PCR. This PCR was validated in two different laboratories with a variety of mycoplasma strains isolated from goats. This new PCR technique will be very useful for a quicker determination of M. putrefaciens strains as well as a better understanding of the prevalence of M. putrefaciens infections. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/517194/1/517194.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.mcp.2003.07.006 10.1016/j.mcp.2003.07.006 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=179543 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mcp.2003.07.006 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.mcp.2003.07.006 |
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L74 - Troubles divers des animaux chèvre PCR mycoplasmose Mycoplasma putrefaciens http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_37783 L74 - Troubles divers des animaux chèvre PCR mycoplasmose Mycoplasma putrefaciens http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_37783 |
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L74 - Troubles divers des animaux chèvre PCR mycoplasmose Mycoplasma putrefaciens http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_37783 L74 - Troubles divers des animaux chèvre PCR mycoplasmose Mycoplasma putrefaciens http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_37783 Peyraud, Armelle Woubit, Salah Poveda, J.B. De La Fé, C. Mercier, Pascale Thiaucourt, François A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats |
description |
Mycoplasma putrefaciens is listed as one of the etiologic agents of the contagious agalactia syndrome by the world organisation for animal health. This species has been characterized only recently, 1974, and the number of outbreaks caused by this microorganism so far is very scarce. It induces mastitis in infected goats although other symptoms such as arthritis in adults and septicaemia in kids are also frequently described. Up to now, the identification of M. putrefaciens relied on classical isolation and identification techniques which present a number of limitations. Specific primers for PCR have been designed based on sequence comparisons of the Arc B gene among the 'Mycoplasma mycoides cluster' and related species such as Mycoplasma cottewii and Mycoplasma yeatsii. Sequence alignments confirmed the taxonomic position of M. putrefaciens, which is related to the W. mycoides cluster' but also very close to M. yeatsii. The polymorphism observed amongst the different Arc B sequences allowed the determination of a primer pair yielding a specific amplification of a 316 bp-long DNA fragment by PCR. This PCR was validated in two different laboratories with a variety of mycoplasma strains isolated from goats. This new PCR technique will be very useful for a quicker determination of M. putrefaciens strains as well as a better understanding of the prevalence of M. putrefaciens infections. |
format |
article |
topic_facet |
L74 - Troubles divers des animaux chèvre PCR mycoplasmose Mycoplasma putrefaciens http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_37783 |
author |
Peyraud, Armelle Woubit, Salah Poveda, J.B. De La Fé, C. Mercier, Pascale Thiaucourt, François |
author_facet |
Peyraud, Armelle Woubit, Salah Poveda, J.B. De La Fé, C. Mercier, Pascale Thiaucourt, François |
author_sort |
Peyraud, Armelle |
title |
A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats |
title_short |
A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats |
title_full |
A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats |
title_fullStr |
A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats |
title_full_unstemmed |
A specific PCR for the detection of Mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats |
title_sort |
specific pcr for the detection of mycoplasma putrefaciens, one of the agents of the contagious agalactia syndrome of goats |
url |
http://agritrop.cirad.fr/517194/ http://agritrop.cirad.fr/517194/1/517194.pdf |
work_keys_str_mv |
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