RT-PCR for a broad detection of pecluviruses

The pecluviruses Peanut clump virus (PCV) and Indian peanut clump virus (IPCV) cause significant losses to crops of groundnut. They are transmitted by the Plasmodiophoromycete Polymyxa graminis and via the seeds. The reported host ranges of these viruses are very wide and include both monocotyledonous and dicotyledonous plants. Clearly, there is a need for integrated control measures for the disease they cause, and these measures would include the use of virus-free groundnut seeds and the limitation of virus spread in soil, weeds and crop species. Virus serological detection is hampered by the substantial differences observed among the amino acid sequences of pecluvirus coat proteins. Therefore, the availability of non-serological, sensitive and broad-spectrum detection methods are essential for the management of clump disease. RT-PCR targeting the 5'-end and 3'-end of the both RNAs of bi-partite PCV and IPCV genome was used for the diagnosis of infection on plant species usually associated with groundnut crops, and compared to the ELISA. Both viruses were detected in the picogram. range in purified preparations and in extracts of infected leaves and roots. Plants suspected to be infected by pecluviruses were collected from India (Bapatla, Hyderabad, Orissa), as well as from Africa (Burkina Faso, Mali, Niger, Senegal). On 199 samples collected at Hyderabad from roots, more than 94% of the ELISA results were similar to RT-PCR and 6% of the samples were positive by RT-PCR only. The RT-PCR proved useful in the detection of the different serotypes and serogroups of the Pecluvirus genus. It detected viruses belonging to each of the three known serotypes of IPCV as well as PCV isolates from Africa collected from 1988 up to 2002. In addition, it gave also positive reaction with samples that had failed to react with any of the available antisera. Positive results were obtained for samples of naturally infected groundnut, durum wheat (Triticum durum) and Bermudagrass (Cynodon dactylon) as well as of mechanically infected Nicotiana benthamiana. The results underline the diversity observed between African isolates in West Africa. They emphasise that pecluviruses are present in graminaceous hosts even when no symptomis apparent and stresses the risk linked with seed exchanges. (Texte intégral)

Saved in:
Bibliographic Details
Main Authors: Bragard, Claude, Hénin, S., Legrève, Anne, Dollet, Michel
Format: conference_item biblioteca
Language:eng
Published: AAB
Subjects:F60 - Physiologie et biochimie végétale, arachide, Myxomycetes, contrôle de maladies, Test ELISA, http://aims.fao.org/aos/agrovoc/c_11368, http://aims.fao.org/aos/agrovoc/c_5057, http://aims.fao.org/aos/agrovoc/c_2327, http://aims.fao.org/aos/agrovoc/c_15968,
Online Access:http://agritrop.cirad.fr/516967/
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-cirad-fr-516967
record_format koha
spelling dig-cirad-fr-5169672024-01-28T11:58:06Z http://agritrop.cirad.fr/516967/ http://agritrop.cirad.fr/516967/ RT-PCR for a broad detection of pecluviruses. Bragard Claude, Hénin S., Legrève Anne, Dollet Michel. 2003. In : Advances in plant virology : A three day International conference at CIRAD, Montpellier, France on 29 September - 1 October 2003. AAB, CIRAD-AMIS, INRA, ENSAM, IRD. Warwick : AAB, Résumé, 1 p. International Conference : Advances in Plant Virology, Montpellier, France, 29 Septembre 2003/1 Octobre 2003. RT-PCR for a broad detection of pecluviruses Bragard, Claude Hénin, S. Legrève, Anne Dollet, Michel eng 2003 AAB Advances in plant virology : A three day International conference at CIRAD, Montpellier, France on 29 September - 1 October 2003 F60 - Physiologie et biochimie végétale arachide Myxomycetes contrôle de maladies Test ELISA http://aims.fao.org/aos/agrovoc/c_11368 http://aims.fao.org/aos/agrovoc/c_5057 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_15968 The pecluviruses Peanut clump virus (PCV) and Indian peanut clump virus (IPCV) cause significant losses to crops of groundnut. They are transmitted by the Plasmodiophoromycete Polymyxa graminis and via the seeds. The reported host ranges of these viruses are very wide and include both monocotyledonous and dicotyledonous plants. Clearly, there is a need for integrated control measures for the disease they cause, and these measures would include the use of virus-free groundnut seeds and the limitation of virus spread in soil, weeds and crop species. Virus serological detection is hampered by the substantial differences observed among the amino acid sequences of pecluvirus coat proteins. Therefore, the availability of non-serological, sensitive and broad-spectrum detection methods are essential for the management of clump disease. RT-PCR targeting the 5'-end and 3'-end of the both RNAs of bi-partite PCV and IPCV genome was used for the diagnosis of infection on plant species usually associated with groundnut crops, and compared to the ELISA. Both viruses were detected in the picogram. range in purified preparations and in extracts of infected leaves and roots. Plants suspected to be infected by pecluviruses were collected from India (Bapatla, Hyderabad, Orissa), as well as from Africa (Burkina Faso, Mali, Niger, Senegal). On 199 samples collected at Hyderabad from roots, more than 94% of the ELISA results were similar to RT-PCR and 6% of the samples were positive by RT-PCR only. The RT-PCR proved useful in the detection of the different serotypes and serogroups of the Pecluvirus genus. It detected viruses belonging to each of the three known serotypes of IPCV as well as PCV isolates from Africa collected from 1988 up to 2002. In addition, it gave also positive reaction with samples that had failed to react with any of the available antisera. Positive results were obtained for samples of naturally infected groundnut, durum wheat (Triticum durum) and Bermudagrass (Cynodon dactylon) as well as of mechanically infected Nicotiana benthamiana. The results underline the diversity observed between African isolates in West Africa. They emphasise that pecluviruses are present in graminaceous hosts even when no symptomis apparent and stresses the risk linked with seed exchanges. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://agritrop.cirad.fr/516952/
institution CIRAD FR
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-cirad-fr
tag biblioteca
region Europa del Oeste
libraryname Biblioteca del CIRAD Francia
language eng
topic F60 - Physiologie et biochimie végétale
arachide
Myxomycetes
contrôle de maladies
Test ELISA
http://aims.fao.org/aos/agrovoc/c_11368
http://aims.fao.org/aos/agrovoc/c_5057
http://aims.fao.org/aos/agrovoc/c_2327
http://aims.fao.org/aos/agrovoc/c_15968
F60 - Physiologie et biochimie végétale
arachide
Myxomycetes
contrôle de maladies
Test ELISA
http://aims.fao.org/aos/agrovoc/c_11368
http://aims.fao.org/aos/agrovoc/c_5057
http://aims.fao.org/aos/agrovoc/c_2327
http://aims.fao.org/aos/agrovoc/c_15968
spellingShingle F60 - Physiologie et biochimie végétale
arachide
Myxomycetes
contrôle de maladies
Test ELISA
http://aims.fao.org/aos/agrovoc/c_11368
http://aims.fao.org/aos/agrovoc/c_5057
http://aims.fao.org/aos/agrovoc/c_2327
http://aims.fao.org/aos/agrovoc/c_15968
F60 - Physiologie et biochimie végétale
arachide
Myxomycetes
contrôle de maladies
Test ELISA
http://aims.fao.org/aos/agrovoc/c_11368
http://aims.fao.org/aos/agrovoc/c_5057
http://aims.fao.org/aos/agrovoc/c_2327
http://aims.fao.org/aos/agrovoc/c_15968
Bragard, Claude
Hénin, S.
Legrève, Anne
Dollet, Michel
RT-PCR for a broad detection of pecluviruses
description The pecluviruses Peanut clump virus (PCV) and Indian peanut clump virus (IPCV) cause significant losses to crops of groundnut. They are transmitted by the Plasmodiophoromycete Polymyxa graminis and via the seeds. The reported host ranges of these viruses are very wide and include both monocotyledonous and dicotyledonous plants. Clearly, there is a need for integrated control measures for the disease they cause, and these measures would include the use of virus-free groundnut seeds and the limitation of virus spread in soil, weeds and crop species. Virus serological detection is hampered by the substantial differences observed among the amino acid sequences of pecluvirus coat proteins. Therefore, the availability of non-serological, sensitive and broad-spectrum detection methods are essential for the management of clump disease. RT-PCR targeting the 5'-end and 3'-end of the both RNAs of bi-partite PCV and IPCV genome was used for the diagnosis of infection on plant species usually associated with groundnut crops, and compared to the ELISA. Both viruses were detected in the picogram. range in purified preparations and in extracts of infected leaves and roots. Plants suspected to be infected by pecluviruses were collected from India (Bapatla, Hyderabad, Orissa), as well as from Africa (Burkina Faso, Mali, Niger, Senegal). On 199 samples collected at Hyderabad from roots, more than 94% of the ELISA results were similar to RT-PCR and 6% of the samples were positive by RT-PCR only. The RT-PCR proved useful in the detection of the different serotypes and serogroups of the Pecluvirus genus. It detected viruses belonging to each of the three known serotypes of IPCV as well as PCV isolates from Africa collected from 1988 up to 2002. In addition, it gave also positive reaction with samples that had failed to react with any of the available antisera. Positive results were obtained for samples of naturally infected groundnut, durum wheat (Triticum durum) and Bermudagrass (Cynodon dactylon) as well as of mechanically infected Nicotiana benthamiana. The results underline the diversity observed between African isolates in West Africa. They emphasise that pecluviruses are present in graminaceous hosts even when no symptomis apparent and stresses the risk linked with seed exchanges. (Texte intégral)
format conference_item
topic_facet F60 - Physiologie et biochimie végétale
arachide
Myxomycetes
contrôle de maladies
Test ELISA
http://aims.fao.org/aos/agrovoc/c_11368
http://aims.fao.org/aos/agrovoc/c_5057
http://aims.fao.org/aos/agrovoc/c_2327
http://aims.fao.org/aos/agrovoc/c_15968
author Bragard, Claude
Hénin, S.
Legrève, Anne
Dollet, Michel
author_facet Bragard, Claude
Hénin, S.
Legrève, Anne
Dollet, Michel
author_sort Bragard, Claude
title RT-PCR for a broad detection of pecluviruses
title_short RT-PCR for a broad detection of pecluviruses
title_full RT-PCR for a broad detection of pecluviruses
title_fullStr RT-PCR for a broad detection of pecluviruses
title_full_unstemmed RT-PCR for a broad detection of pecluviruses
title_sort rt-pcr for a broad detection of pecluviruses
publisher AAB
url http://agritrop.cirad.fr/516967/
work_keys_str_mv AT bragardclaude rtpcrforabroaddetectionofpecluviruses
AT henins rtpcrforabroaddetectionofpecluviruses
AT legreveanne rtpcrforabroaddetectionofpecluviruses
AT dolletmichel rtpcrforabroaddetectionofpecluviruses
_version_ 1792495709493133312