Association studies using random and candidate microsatellite loci in two infectious goat diseases
We established a set of 30 microsatellites of Bovidae origin for use in a biodiversity study in Swiss and Creole goats. Additional microsatellites located within or next to "candidate" genes of interest, such as cytokine genes (IL4, INF-gamma) and MHC class II genes (DRB, DYA) were tested in the caprine species in order to detect possible associations with two infectious caprine diseases. Microsatellite analysis was undertaken using automated sequencers (ABI373 & 3100). In the first study, a total of 82 unrelated Creole goats, 37 resistant and 45 susceptible to Heartwater disease (Cowdriosis) were analysed. In this study, the two microsatellite loci DRBPI (MHCII) and BOBT24 (IL4) were positively associated with disease susceptibility, demonstrating a corrected P-value of 0.002 and 0.005, respectively. In a second investigation, we tested 36 goats, naturally infected with the nematode parasite Trichostrongylus colubriformis. These animals were divided into a "low" and "high" excreting group on the basis of two independently recorded fecal egg counts. For this nematode resistance study, we detected a significant association of one of the alleles of the microsatellite locus SPS113 with "low" excretion (resistance). The MHC class II locus DYA (P19), was weakly associated with susceptibility in both diseases (Pc = 0.05). In future experiments, we will extend the sample size in order to verify the described associations.
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Subjects: | L10 - Génétique et amélioration des animaux, maladie infectieuse, chèvre, microsatellite, génétique moléculaire, résistance aux maladies, http://aims.fao.org/aos/agrovoc/c_34024, http://aims.fao.org/aos/agrovoc/c_5066, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_27577, http://aims.fao.org/aos/agrovoc/c_2328, |
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dig-cirad-fr-5153852024-01-28T11:44:48Z http://agritrop.cirad.fr/515385/ http://agritrop.cirad.fr/515385/ Association studies using random and candidate microsatellite loci in two infectious goat diseases. Obexer-Ruff Gabriela, Sattler Ursula, Martinez Dominique, Maillard Jean-Charles, Chartier Christophe, Saitbekova Nasikat, Glowatzki Marie-Louise, Gaillard Claude. 2003. Genetics Selection Evolution, 35suppl.1, S113-S119 International Symposium on Candidate Genes for Animal Health. 2, Montpellier, France, 16 Août 2002/18 Août 2002.https://doi.org/10.1051/gse:2003020 <https://doi.org/10.1051/gse:2003020> Association studies using random and candidate microsatellite loci in two infectious goat diseases Obexer-Ruff, Gabriela Sattler, Ursula Martinez, Dominique Maillard, Jean-Charles Chartier, Christophe Saitbekova, Nasikat Glowatzki, Marie-Louise Gaillard, Claude eng 2003 Genetics Selection Evolution L10 - Génétique et amélioration des animaux maladie infectieuse chèvre microsatellite génétique moléculaire résistance aux maladies http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_2328 We established a set of 30 microsatellites of Bovidae origin for use in a biodiversity study in Swiss and Creole goats. Additional microsatellites located within or next to "candidate" genes of interest, such as cytokine genes (IL4, INF-gamma) and MHC class II genes (DRB, DYA) were tested in the caprine species in order to detect possible associations with two infectious caprine diseases. Microsatellite analysis was undertaken using automated sequencers (ABI373 & 3100). In the first study, a total of 82 unrelated Creole goats, 37 resistant and 45 susceptible to Heartwater disease (Cowdriosis) were analysed. In this study, the two microsatellite loci DRBPI (MHCII) and BOBT24 (IL4) were positively associated with disease susceptibility, demonstrating a corrected P-value of 0.002 and 0.005, respectively. In a second investigation, we tested 36 goats, naturally infected with the nematode parasite Trichostrongylus colubriformis. These animals were divided into a "low" and "high" excreting group on the basis of two independently recorded fecal egg counts. For this nematode resistance study, we detected a significant association of one of the alleles of the microsatellite locus SPS113 with "low" excretion (resistance). The MHC class II locus DYA (P19), was weakly associated with susceptibility in both diseases (Pc = 0.05). In future experiments, we will extend the sample size in order to verify the described associations. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/515385/1/ID_515385.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1051/gse:2003020 10.1051/gse:2003020 info:eu-repo/semantics/altIdentifier/doi/10.1051/gse:2003020 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1051/gse:2003020 |
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L10 - Génétique et amélioration des animaux maladie infectieuse chèvre microsatellite génétique moléculaire résistance aux maladies http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_2328 L10 - Génétique et amélioration des animaux maladie infectieuse chèvre microsatellite génétique moléculaire résistance aux maladies http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_2328 |
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L10 - Génétique et amélioration des animaux maladie infectieuse chèvre microsatellite génétique moléculaire résistance aux maladies http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_2328 L10 - Génétique et amélioration des animaux maladie infectieuse chèvre microsatellite génétique moléculaire résistance aux maladies http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_2328 Obexer-Ruff, Gabriela Sattler, Ursula Martinez, Dominique Maillard, Jean-Charles Chartier, Christophe Saitbekova, Nasikat Glowatzki, Marie-Louise Gaillard, Claude Association studies using random and candidate microsatellite loci in two infectious goat diseases |
description |
We established a set of 30 microsatellites of Bovidae origin for use in a biodiversity study in Swiss and Creole goats. Additional microsatellites located within or next to "candidate" genes of interest, such as cytokine genes (IL4, INF-gamma) and MHC class II genes (DRB, DYA) were tested in the caprine species in order to detect possible associations with two infectious caprine diseases. Microsatellite analysis was undertaken using automated sequencers (ABI373 & 3100). In the first study, a total of 82 unrelated Creole goats, 37 resistant and 45 susceptible to Heartwater disease (Cowdriosis) were analysed. In this study, the two microsatellite loci DRBPI (MHCII) and BOBT24 (IL4) were positively associated with disease susceptibility, demonstrating a corrected P-value of 0.002 and 0.005, respectively. In a second investigation, we tested 36 goats, naturally infected with the nematode parasite Trichostrongylus colubriformis. These animals were divided into a "low" and "high" excreting group on the basis of two independently recorded fecal egg counts. For this nematode resistance study, we detected a significant association of one of the alleles of the microsatellite locus SPS113 with "low" excretion (resistance). The MHC class II locus DYA (P19), was weakly associated with susceptibility in both diseases (Pc = 0.05). In future experiments, we will extend the sample size in order to verify the described associations. |
format |
article |
topic_facet |
L10 - Génétique et amélioration des animaux maladie infectieuse chèvre microsatellite génétique moléculaire résistance aux maladies http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_5066 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_2328 |
author |
Obexer-Ruff, Gabriela Sattler, Ursula Martinez, Dominique Maillard, Jean-Charles Chartier, Christophe Saitbekova, Nasikat Glowatzki, Marie-Louise Gaillard, Claude |
author_facet |
Obexer-Ruff, Gabriela Sattler, Ursula Martinez, Dominique Maillard, Jean-Charles Chartier, Christophe Saitbekova, Nasikat Glowatzki, Marie-Louise Gaillard, Claude |
author_sort |
Obexer-Ruff, Gabriela |
title |
Association studies using random and candidate microsatellite loci in two infectious goat diseases |
title_short |
Association studies using random and candidate microsatellite loci in two infectious goat diseases |
title_full |
Association studies using random and candidate microsatellite loci in two infectious goat diseases |
title_fullStr |
Association studies using random and candidate microsatellite loci in two infectious goat diseases |
title_full_unstemmed |
Association studies using random and candidate microsatellite loci in two infectious goat diseases |
title_sort |
association studies using random and candidate microsatellite loci in two infectious goat diseases |
url |
http://agritrop.cirad.fr/515385/ http://agritrop.cirad.fr/515385/1/ID_515385.pdf |
work_keys_str_mv |
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_version_ |
1792495645689380864 |