Expression of a maize proteinase inhibitor in rice plants enhances resistance toward Chilo supressalis larvae [M-12]
The mpi gene encodes a proteinase inhibitor, the MPI (Maize Proteinase Inhibitor) protein, whose expression is induced in response to mechanical wounding and insect feeding in maize plants. MPI was found to be an effective inhibitor of elastase and chymotrypsin activities from the larval midgut of the lepidopteran insect Spodoptera littoralis. In this study, the mpi gene was expressed in rice plants either under the control of the maize-1 ubiquitin promoter or under the control of its own promoter and 3' terminator sequences. MPI was identified in protein extracts from transgenic rice plants by immunoblotting. The stability of transgene expression through successive generations of mpi rice plants was confirmed. Insect feeding experiments revealed that transgenic expression of mpi in rice enhances resistance against striped stem borer (SSB) (Chilo suppressalis Walker), one of the major insect pest of rice. Growth and development of C. suppressalis larvae fed on leaves of rice plants harbouring the mpi gene was significantly retarded, compared to growth of larvae fed in control untransformed leaves. Analysis of proteinase activities present in the gut of C. suppressalis larvae fed on mpi rice plants showed an increase in the total proteolytic activity. The observed overproduction of proteinases is, however, not effective to compensate the inhibitory effect of MPI on the digestive proteolytic activities of C. suppressalis. (Texte intégral)
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Subjects: | F30 - Génétique et amélioration des plantes, inhibiteur de protéinases, expression des gènes, Zea mays, plante transgénique, résistance aux organismes nuisibles, Chilo suppressalis, génie génétique, http://aims.fao.org/aos/agrovoc/c_36656, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_8504, http://aims.fao.org/aos/agrovoc/c_27619, http://aims.fao.org/aos/agrovoc/c_5731, http://aims.fao.org/aos/agrovoc/c_27157, http://aims.fao.org/aos/agrovoc/c_15974, |
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dig-cirad-fr-5140212024-01-28T11:28:05Z http://agritrop.cirad.fr/514021/ http://agritrop.cirad.fr/514021/ Expression of a maize proteinase inhibitor in rice plants enhances resistance toward Chilo supressalis larvae [M-12]. Vila Laura, Meynard Donaldo, Breitler Jean-Christophe, Quilis Jordi, Marfa Victoria, Vassal Jean-Michel, Guiderdoni Emmanuel, San Segundo Blanca. 2003. In : 7th International Congress of Plant Molecular Biology, ISPMB 2003, Barcelona (Spain), June 23-28. Book of abstracts. ISPMB. Barcelone : ISPMB Office, Résumé, 431. International Congress of Plant Molecular Biology. 7, Barcelone, Espagne, 23 Juin 2003/28 Juin 2003. Expression of a maize proteinase inhibitor in rice plants enhances resistance toward Chilo supressalis larvae [M-12] Vila, Laura Meynard, Donaldo Breitler, Jean-Christophe Quilis, Jordi Marfa, Victoria Vassal, Jean-Michel Guiderdoni, Emmanuel San Segundo, Blanca eng 2003 ISPMB Office 7th International Congress of Plant Molecular Biology, ISPMB 2003, Barcelona (Spain), June 23-28. Book of abstracts F30 - Génétique et amélioration des plantes inhibiteur de protéinases expression des gènes Zea mays plante transgénique résistance aux organismes nuisibles Chilo suppressalis génie génétique http://aims.fao.org/aos/agrovoc/c_36656 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_15974 The mpi gene encodes a proteinase inhibitor, the MPI (Maize Proteinase Inhibitor) protein, whose expression is induced in response to mechanical wounding and insect feeding in maize plants. MPI was found to be an effective inhibitor of elastase and chymotrypsin activities from the larval midgut of the lepidopteran insect Spodoptera littoralis. In this study, the mpi gene was expressed in rice plants either under the control of the maize-1 ubiquitin promoter or under the control of its own promoter and 3' terminator sequences. MPI was identified in protein extracts from transgenic rice plants by immunoblotting. The stability of transgene expression through successive generations of mpi rice plants was confirmed. Insect feeding experiments revealed that transgenic expression of mpi in rice enhances resistance against striped stem borer (SSB) (Chilo suppressalis Walker), one of the major insect pest of rice. Growth and development of C. suppressalis larvae fed on leaves of rice plants harbouring the mpi gene was significantly retarded, compared to growth of larvae fed in control untransformed leaves. Analysis of proteinase activities present in the gut of C. suppressalis larvae fed on mpi rice plants showed an increase in the total proteolytic activity. The observed overproduction of proteinases is, however, not effective to compensate the inhibitory effect of MPI on the digestive proteolytic activities of C. suppressalis. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=176945 |
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F30 - Génétique et amélioration des plantes inhibiteur de protéinases expression des gènes Zea mays plante transgénique résistance aux organismes nuisibles Chilo suppressalis génie génétique http://aims.fao.org/aos/agrovoc/c_36656 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_15974 F30 - Génétique et amélioration des plantes inhibiteur de protéinases expression des gènes Zea mays plante transgénique résistance aux organismes nuisibles Chilo suppressalis génie génétique http://aims.fao.org/aos/agrovoc/c_36656 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_15974 |
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F30 - Génétique et amélioration des plantes inhibiteur de protéinases expression des gènes Zea mays plante transgénique résistance aux organismes nuisibles Chilo suppressalis génie génétique http://aims.fao.org/aos/agrovoc/c_36656 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_15974 F30 - Génétique et amélioration des plantes inhibiteur de protéinases expression des gènes Zea mays plante transgénique résistance aux organismes nuisibles Chilo suppressalis génie génétique http://aims.fao.org/aos/agrovoc/c_36656 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_15974 Vila, Laura Meynard, Donaldo Breitler, Jean-Christophe Quilis, Jordi Marfa, Victoria Vassal, Jean-Michel Guiderdoni, Emmanuel San Segundo, Blanca Expression of a maize proteinase inhibitor in rice plants enhances resistance toward Chilo supressalis larvae [M-12] |
description |
The mpi gene encodes a proteinase inhibitor, the MPI (Maize Proteinase Inhibitor) protein, whose expression is induced in response to mechanical wounding and insect feeding in maize plants. MPI was found to be an effective inhibitor of elastase and chymotrypsin activities from the larval midgut of the lepidopteran insect Spodoptera littoralis. In this study, the mpi gene was expressed in rice plants either under the control of the maize-1 ubiquitin promoter or under the control of its own promoter and 3' terminator sequences. MPI was identified in protein extracts from transgenic rice plants by immunoblotting. The stability of transgene expression through successive generations of mpi rice plants was confirmed. Insect feeding experiments revealed that transgenic expression of mpi in rice enhances resistance against striped stem borer (SSB) (Chilo suppressalis Walker), one of the major insect pest of rice. Growth and development of C. suppressalis larvae fed on leaves of rice plants harbouring the mpi gene was significantly retarded, compared to growth of larvae fed in control untransformed leaves. Analysis of proteinase activities present in the gut of C. suppressalis larvae fed on mpi rice plants showed an increase in the total proteolytic activity. The observed overproduction of proteinases is, however, not effective to compensate the inhibitory effect of MPI on the digestive proteolytic activities of C. suppressalis. (Texte intégral) |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes inhibiteur de protéinases expression des gènes Zea mays plante transgénique résistance aux organismes nuisibles Chilo suppressalis génie génétique http://aims.fao.org/aos/agrovoc/c_36656 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_15974 |
author |
Vila, Laura Meynard, Donaldo Breitler, Jean-Christophe Quilis, Jordi Marfa, Victoria Vassal, Jean-Michel Guiderdoni, Emmanuel San Segundo, Blanca |
author_facet |
Vila, Laura Meynard, Donaldo Breitler, Jean-Christophe Quilis, Jordi Marfa, Victoria Vassal, Jean-Michel Guiderdoni, Emmanuel San Segundo, Blanca |
author_sort |
Vila, Laura |
title |
Expression of a maize proteinase inhibitor in rice plants enhances resistance toward Chilo supressalis larvae [M-12] |
title_short |
Expression of a maize proteinase inhibitor in rice plants enhances resistance toward Chilo supressalis larvae [M-12] |
title_full |
Expression of a maize proteinase inhibitor in rice plants enhances resistance toward Chilo supressalis larvae [M-12] |
title_fullStr |
Expression of a maize proteinase inhibitor in rice plants enhances resistance toward Chilo supressalis larvae [M-12] |
title_full_unstemmed |
Expression of a maize proteinase inhibitor in rice plants enhances resistance toward Chilo supressalis larvae [M-12] |
title_sort |
expression of a maize proteinase inhibitor in rice plants enhances resistance toward chilo supressalis larvae [m-12] |
publisher |
ISPMB Office |
url |
http://agritrop.cirad.fr/514021/ |
work_keys_str_mv |
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