Production of haploids from anther culture of banana (Musa balbisiana (BB))

We report here, for the first time, the production of haploid plants of banana #Musa balbisiana# (BB). Callus was induced from anthers in which the majority of the microspores were at the uninucleate stage. The frequency of callus induction was 77%. Callus proliferation usually preceded embryo formation. About 8% of the anthers developed androgenic embryos. Of the 147 plantlets obtained, 41 were haploids (n=x=11). The frequency of haploid production depended on genotypes used: 18 haploid plants were produced from genotype 'Pisang klutuk', 12 from 'Pisang batu', seven from 'Pisang klutuk wulung' and four from 'Tani'. The frequency of regeneration was 1.1%, which was based on the total number of anthers cultured. Diploid plants (2n=2x=22) were also observed in the regenerated plants. The haploid banana plants that were developed will be important material for the improvement of banana through breeding programmes.

Bibliographic Details

Main Authors:	Assani, A., Bakry, Frédéric, Kerbellec, Florence, Haïcour, R., Wenzel, Gerhard, Foroughi-Wehr, Barbel
Format:	article biblioteca
Language:	eng
Subjects:	F02 - Multiplication végétative des plantes, F30 - Génétique et amélioration des plantes, Musa balbisiana, haploïdie, culture d'anthère, androgénèse, régénération in vitro, nombre chromosomique, amélioration des plantes, http://aims.fao.org/aos/agrovoc/c_4995, http://aims.fao.org/aos/agrovoc/c_3490, http://aims.fao.org/aos/agrovoc/c_8837, http://aims.fao.org/aos/agrovoc/c_25421, http://aims.fao.org/aos/agrovoc/c_34114, http://aims.fao.org/aos/agrovoc/c_1597, http://aims.fao.org/aos/agrovoc/c_5956,
Online Access:	http://agritrop.cirad.fr/513229/ http://agritrop.cirad.fr/513229/1/ID513229.pdf
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