Is DNA methylation underlying somaclonal variation in oil palm?
The role of DNA methylation in the regulation of gene expression and its implication in somaclonal variation has been extensively investigated. Significant changes in the methylation level of genomic DNA during dedifferentiation and somatic embryogenesis have been reported for higher plants. The occurrence of somaclonal variants (ca 5%) among populations of somatic embryo-derived oil palms (Elaeis guineensis Jacq.) currently hampers the scaling-up of clonal plant production. Variant "mantled" palms show a feminisation of flower male parts, which can result in complete sterility in the most severe cases. Given the observed features of the abnormality (spatial heterogeneity, temporal instability, non-Mendelian sexual transmission, absence of any detectable defect in DNA organisation as revealed by AFLP/RAPD studies) the hypothesis of an epigenetic alteration of genome expression has been proposed to explain the origin of the variant plants. The relationship between the "mantled" variation and hypomethylation of genomic DNA has been assessed, using two complementary approaches: HPLC quantification of relative amounts of 5-methyl-deoxycytidine and the Methylase Accepting Assay (MAA), based on the enzymatic saturation of CG sites with methyl groups (Jaligot et al., 2000). RFLP and AFLP studies are currently implemented, using isoschizomeric restriction enzyme pairs showing differential sensitivity to the methylation of dC residues (eg MspI and HpaII). As active chromatin may show reduced methylation rates as compared with its inactive counterpart, we are now comparing methylation rates in DNA corresponding to active chromatin in normal and variant regenerants.
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Subjects: | F02 - Multiplication végétative des plantes, F30 - Génétique et amélioration des plantes, Elaeis guineensis, clone, Embryogénèse somatique, adn, méthylation, génome, variation somaclonale, trouble de différenciation sexuelle, RFLP, PCR, chromatine, analyse enzymatique, http://aims.fao.org/aos/agrovoc/c_2509, http://aims.fao.org/aos/agrovoc/c_1678, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_24872, http://aims.fao.org/aos/agrovoc/c_3224, http://aims.fao.org/aos/agrovoc/c_34127, http://aims.fao.org/aos/agrovoc/c_7012, http://aims.fao.org/aos/agrovoc/c_34255, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_1593, http://aims.fao.org/aos/agrovoc/c_26867, |
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dig-cirad-fr-5101332024-01-28T10:47:00Z http://agritrop.cirad.fr/510133/ http://agritrop.cirad.fr/510133/ Is DNA methylation underlying somaclonal variation in oil palm? Rival Alain, Jaligot Estelle, Laroche A., Beulé Thierry, Oakeley E.J., Verdeil Jean-Luc, Tregear James. 2002. In : Proceedings of the International Symposium on Tropical and Subtropical Fruits. Drew R.A. (ed.). ISHS. Wageningen : ISHS, 47-59. (Acta Horticulturae, 575) ISBN 90-6605-885-4 International Symposium on Tropical and Subtropical Fruits, Cairns, Australie, 26 Novembre 2000/1 Décembre 2000. Is DNA methylation underlying somaclonal variation in oil palm? Rival, Alain Jaligot, Estelle Laroche, A. Beulé, Thierry Oakeley, E.J. Verdeil, Jean-Luc Tregear, James eng 2002 ISHS Proceedings of the International Symposium on Tropical and Subtropical Fruits F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Elaeis guineensis clone Embryogénèse somatique adn méthylation génome variation somaclonale trouble de différenciation sexuelle RFLP PCR chromatine analyse enzymatique http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_7012 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1593 http://aims.fao.org/aos/agrovoc/c_26867 The role of DNA methylation in the regulation of gene expression and its implication in somaclonal variation has been extensively investigated. Significant changes in the methylation level of genomic DNA during dedifferentiation and somatic embryogenesis have been reported for higher plants. The occurrence of somaclonal variants (ca 5%) among populations of somatic embryo-derived oil palms (Elaeis guineensis Jacq.) currently hampers the scaling-up of clonal plant production. Variant "mantled" palms show a feminisation of flower male parts, which can result in complete sterility in the most severe cases. Given the observed features of the abnormality (spatial heterogeneity, temporal instability, non-Mendelian sexual transmission, absence of any detectable defect in DNA organisation as revealed by AFLP/RAPD studies) the hypothesis of an epigenetic alteration of genome expression has been proposed to explain the origin of the variant plants. The relationship between the "mantled" variation and hypomethylation of genomic DNA has been assessed, using two complementary approaches: HPLC quantification of relative amounts of 5-methyl-deoxycytidine and the Methylase Accepting Assay (MAA), based on the enzymatic saturation of CG sites with methyl groups (Jaligot et al., 2000). RFLP and AFLP studies are currently implemented, using isoschizomeric restriction enzyme pairs showing differential sensitivity to the methylation of dC residues (eg MspI and HpaII). As active chromatin may show reduced methylation rates as compared with its inactive counterpart, we are now comparing methylation rates in DNA corresponding to active chromatin in normal and variant regenerants. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=174629 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=176254 |
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F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Elaeis guineensis clone Embryogénèse somatique adn méthylation génome variation somaclonale trouble de différenciation sexuelle RFLP PCR chromatine analyse enzymatique http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_7012 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1593 http://aims.fao.org/aos/agrovoc/c_26867 F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Elaeis guineensis clone Embryogénèse somatique adn méthylation génome variation somaclonale trouble de différenciation sexuelle RFLP PCR chromatine analyse enzymatique http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_7012 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1593 http://aims.fao.org/aos/agrovoc/c_26867 |
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F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Elaeis guineensis clone Embryogénèse somatique adn méthylation génome variation somaclonale trouble de différenciation sexuelle RFLP PCR chromatine analyse enzymatique http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_7012 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1593 http://aims.fao.org/aos/agrovoc/c_26867 F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Elaeis guineensis clone Embryogénèse somatique adn méthylation génome variation somaclonale trouble de différenciation sexuelle RFLP PCR chromatine analyse enzymatique http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_7012 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1593 http://aims.fao.org/aos/agrovoc/c_26867 Rival, Alain Jaligot, Estelle Laroche, A. Beulé, Thierry Oakeley, E.J. Verdeil, Jean-Luc Tregear, James Is DNA methylation underlying somaclonal variation in oil palm? |
description |
The role of DNA methylation in the regulation of gene expression and its implication in somaclonal variation has been extensively investigated. Significant changes in the methylation level of genomic DNA during dedifferentiation and somatic embryogenesis have been reported for higher plants. The occurrence of somaclonal variants (ca 5%) among populations of somatic embryo-derived oil palms (Elaeis guineensis Jacq.) currently hampers the scaling-up of clonal plant production. Variant "mantled" palms show a feminisation of flower male parts, which can result in complete sterility in the most severe cases. Given the observed features of the abnormality (spatial heterogeneity, temporal instability, non-Mendelian sexual transmission, absence of any detectable defect in DNA organisation as revealed by AFLP/RAPD studies) the hypothesis of an epigenetic alteration of genome expression has been proposed to explain the origin of the variant plants. The relationship between the "mantled" variation and hypomethylation of genomic DNA has been assessed, using two complementary approaches: HPLC quantification of relative amounts of 5-methyl-deoxycytidine and the Methylase Accepting Assay (MAA), based on the enzymatic saturation of CG sites with methyl groups (Jaligot et al., 2000). RFLP and AFLP studies are currently implemented, using isoschizomeric restriction enzyme pairs showing differential sensitivity to the methylation of dC residues (eg MspI and HpaII). As active chromatin may show reduced methylation rates as compared with its inactive counterpart, we are now comparing methylation rates in DNA corresponding to active chromatin in normal and variant regenerants. |
format |
conference_item |
topic_facet |
F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Elaeis guineensis clone Embryogénèse somatique adn méthylation génome variation somaclonale trouble de différenciation sexuelle RFLP PCR chromatine analyse enzymatique http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1678 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_7012 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_1593 http://aims.fao.org/aos/agrovoc/c_26867 |
author |
Rival, Alain Jaligot, Estelle Laroche, A. Beulé, Thierry Oakeley, E.J. Verdeil, Jean-Luc Tregear, James |
author_facet |
Rival, Alain Jaligot, Estelle Laroche, A. Beulé, Thierry Oakeley, E.J. Verdeil, Jean-Luc Tregear, James |
author_sort |
Rival, Alain |
title |
Is DNA methylation underlying somaclonal variation in oil palm? |
title_short |
Is DNA methylation underlying somaclonal variation in oil palm? |
title_full |
Is DNA methylation underlying somaclonal variation in oil palm? |
title_fullStr |
Is DNA methylation underlying somaclonal variation in oil palm? |
title_full_unstemmed |
Is DNA methylation underlying somaclonal variation in oil palm? |
title_sort |
is dna methylation underlying somaclonal variation in oil palm? |
publisher |
ISHS |
url |
http://agritrop.cirad.fr/510133/ |
work_keys_str_mv |
AT rivalalain isdnamethylationunderlyingsomaclonalvariationinoilpalm AT jaligotestelle isdnamethylationunderlyingsomaclonalvariationinoilpalm AT larochea isdnamethylationunderlyingsomaclonalvariationinoilpalm AT beulethierry isdnamethylationunderlyingsomaclonalvariationinoilpalm AT oakeleyej isdnamethylationunderlyingsomaclonalvariationinoilpalm AT verdeiljeanluc isdnamethylationunderlyingsomaclonalvariationinoilpalm AT tregearjames isdnamethylationunderlyingsomaclonalvariationinoilpalm |
_version_ |
1792495372876120064 |