PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats
Trypanosoma (Herpetosoma) lewisi is a trypanosome of the sub-genus Herpetosoma (Stercoraria section), parasite of rats (Rattus rattus and Rattus norvegicus) transmitted by fleas, T. lewisi has a stringent species specificity and cannot grow in other rodents such as mice. Rats are infected principally by oral route, through contamination by flea faeces or ingestion of fleas. Trypanosoma lewisi infections in rat colonies can interfere with research protocols and fleas of wild rats are often the source of such infections, Currently, diagnosis of T. lewisi in rats is performed by microscopic observation of stained blood smears. In the course of a research project at CIRDES, a T. lewisi infection was detected in the rat colony. In this study we evaluated PCR primer sets for their ability to diagnose multiple species of trypanosomes with a single amplification, We show that the use of ITSI sequence of ribosomal DNA provides an efficient and sensitive assay for detection and identification of T. lewisi infection in rats and recommend the use of this assay for monitoring of T. lewisi infections in rat colonies.
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dig-cirad-fr-5088622022-04-21T16:23:07Z http://agritrop.cirad.fr/508862/ http://agritrop.cirad.fr/508862/ PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats. Desquesnes Marc, Ravel Sophie, Cuny Gérard. 2002. Kinetoplastid Biology and Disease, 1 (2), 6 p.https://doi.org/10.1186/1475-9292-1-2 <https://doi.org/10.1186/1475-9292-1-2> Researchers PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats Desquesnes, Marc Ravel, Sophie Cuny, Gérard eng 2002 Kinetoplastid Biology and Disease L73 - Maladies des animaux Trypanosoma (Herpetosoma) lewisi is a trypanosome of the sub-genus Herpetosoma (Stercoraria section), parasite of rats (Rattus rattus and Rattus norvegicus) transmitted by fleas, T. lewisi has a stringent species specificity and cannot grow in other rodents such as mice. Rats are infected principally by oral route, through contamination by flea faeces or ingestion of fleas. Trypanosoma lewisi infections in rat colonies can interfere with research protocols and fleas of wild rats are often the source of such infections, Currently, diagnosis of T. lewisi in rats is performed by microscopic observation of stained blood smears. In the course of a research project at CIRDES, a T. lewisi infection was detected in the rat colony. In this study we evaluated PCR primer sets for their ability to diagnose multiple species of trypanosomes with a single amplification, We show that the use of ITSI sequence of ribosomal DNA provides an efficient and sensitive assay for detection and identification of T. lewisi infection in rats and recommend the use of this assay for monitoring of T. lewisi infections in rat colonies. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/508862/1/document_508862.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1186/1475-9292-1-2 10.1186/1475-9292-1-2 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=173510 info:eu-repo/semantics/altIdentifier/doi/10.1186/1475-9292-1-2 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/1475-9292-1-2 |
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L73 - Maladies des animaux L73 - Maladies des animaux Desquesnes, Marc Ravel, Sophie Cuny, Gérard PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats |
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Trypanosoma (Herpetosoma) lewisi is a trypanosome of the sub-genus Herpetosoma (Stercoraria section), parasite of rats (Rattus rattus and Rattus norvegicus) transmitted by fleas, T. lewisi has a stringent species specificity and cannot grow in other rodents such as mice. Rats are infected principally by oral route, through contamination by flea faeces or ingestion of fleas. Trypanosoma lewisi infections in rat colonies can interfere with research protocols and fleas of wild rats are often the source of such infections, Currently, diagnosis of T. lewisi in rats is performed by microscopic observation of stained blood smears. In the course of a research project at CIRDES, a T. lewisi infection was detected in the rat colony. In this study we evaluated PCR primer sets for their ability to diagnose multiple species of trypanosomes with a single amplification, We show that the use of ITSI sequence of ribosomal DNA provides an efficient and sensitive assay for detection and identification of T. lewisi infection in rats and recommend the use of this assay for monitoring of T. lewisi infections in rat colonies. |
format |
article |
topic_facet |
L73 - Maladies des animaux |
author |
Desquesnes, Marc Ravel, Sophie Cuny, Gérard |
author_facet |
Desquesnes, Marc Ravel, Sophie Cuny, Gérard |
author_sort |
Desquesnes, Marc |
title |
PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats |
title_short |
PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats |
title_full |
PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats |
title_fullStr |
PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats |
title_full_unstemmed |
PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats |
title_sort |
pcr identification of trypanosoma lewisi, a common parasite of laboratory rats |
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http://agritrop.cirad.fr/508862/ http://agritrop.cirad.fr/508862/1/document_508862.pdf |
work_keys_str_mv |
AT desquesnesmarc pcridentificationoftrypanosomalewisiacommonparasiteoflaboratoryrats AT ravelsophie pcridentificationoftrypanosomalewisiacommonparasiteoflaboratoryrats AT cunygerard pcridentificationoftrypanosomalewisiacommonparasiteoflaboratoryrats |
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1758019524886528000 |