Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize
In order to develop a resistance management strategy to control tropical pests based on the co-expression of different toxins, a fully modified Bacillus thuringiensis cry1B gene and the translational fusion cry1B-cry1Ab gene have been developed. Both constructs were cloned under the control of a maize ubiquitin-1 or a rice actin-1 promoter and linked to the bar gene driven by the CaMV 35S promoter. Immature embryos from the tropical lines CML72, CML216, and their hybrids, were used as the target for transformation by microprojectile bombardment. Twenty five percent of the transformed maize plants with cry1B expressed a protein that is active against southwestern corn borer and sugarcane borer. Ten percent of the transgenic maize expressed single fusion proteins from the translational fusion gene cry1B-1Ab and showed resistance to these two pests as well as to the fall armyworm. Transgenic maize plants that carried the cry1B gene in T1 to T3 progenies transmitted trangenes with expected Mendelian segregation and conferred resistance to the two target insects. Molecular analyses confirmed the cry genes integration, the copy number, the size of protein(s) expressed in maize plants, the transmission, and the inheritance of the introduced cry gene. These new transgenic products will provide another recourse for reducing the build-up of resistance in pest populations.
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Subjects: | F30 - Génétique et amélioration des plantes, Zea mays, plante transgénique, Bacillus thuringiensis, gène, résistance aux organismes nuisibles, Diatraea grandiosella, Spodoptera frugiperda, Diatraea saccharalis, http://aims.fao.org/aos/agrovoc/c_8504, http://aims.fao.org/aos/agrovoc/c_27619, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_5731, http://aims.fao.org/aos/agrovoc/c_30309, http://aims.fao.org/aos/agrovoc/c_25452, http://aims.fao.org/aos/agrovoc/c_30310, |
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dig-cirad-fr-4871642024-01-28T10:03:54Z http://agritrop.cirad.fr/487164/ http://agritrop.cirad.fr/487164/ Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize. Bohorova N., Frutos Roger, Royer Monique, Estanol Pilar, Pacheco M., Rascon Q., McLean S., Hoisington David. 2001. Theoretical and Applied Genetics, 103 : 817-826.https://doi.org/10.1007/s001220100686 <https://doi.org/10.1007/s001220100686> Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize Bohorova, N. Frutos, Roger Royer, Monique Estanol, Pilar Pacheco, M. Rascon, Q. McLean, S. Hoisington, David eng 2001 Theoretical and Applied Genetics F30 - Génétique et amélioration des plantes Zea mays plante transgénique Bacillus thuringiensis gène résistance aux organismes nuisibles Diatraea grandiosella Spodoptera frugiperda Diatraea saccharalis http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_30309 http://aims.fao.org/aos/agrovoc/c_25452 http://aims.fao.org/aos/agrovoc/c_30310 In order to develop a resistance management strategy to control tropical pests based on the co-expression of different toxins, a fully modified Bacillus thuringiensis cry1B gene and the translational fusion cry1B-cry1Ab gene have been developed. Both constructs were cloned under the control of a maize ubiquitin-1 or a rice actin-1 promoter and linked to the bar gene driven by the CaMV 35S promoter. Immature embryos from the tropical lines CML72, CML216, and their hybrids, were used as the target for transformation by microprojectile bombardment. Twenty five percent of the transformed maize plants with cry1B expressed a protein that is active against southwestern corn borer and sugarcane borer. Ten percent of the transgenic maize expressed single fusion proteins from the translational fusion gene cry1B-1Ab and showed resistance to these two pests as well as to the fall armyworm. Transgenic maize plants that carried the cry1B gene in T1 to T3 progenies transmitted trangenes with expected Mendelian segregation and conferred resistance to the two target insects. Molecular analyses confirmed the cry genes integration, the copy number, the size of protein(s) expressed in maize plants, the transmission, and the inheritance of the introduced cry gene. These new transgenic products will provide another recourse for reducing the build-up of resistance in pest populations. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/487164/1/487164.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s001220100686 10.1007/s001220100686 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=170546 info:eu-repo/semantics/altIdentifier/doi/10.1007/s001220100686 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s001220100686 |
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F30 - Génétique et amélioration des plantes Zea mays plante transgénique Bacillus thuringiensis gène résistance aux organismes nuisibles Diatraea grandiosella Spodoptera frugiperda Diatraea saccharalis http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_30309 http://aims.fao.org/aos/agrovoc/c_25452 http://aims.fao.org/aos/agrovoc/c_30310 F30 - Génétique et amélioration des plantes Zea mays plante transgénique Bacillus thuringiensis gène résistance aux organismes nuisibles Diatraea grandiosella Spodoptera frugiperda Diatraea saccharalis http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_30309 http://aims.fao.org/aos/agrovoc/c_25452 http://aims.fao.org/aos/agrovoc/c_30310 |
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F30 - Génétique et amélioration des plantes Zea mays plante transgénique Bacillus thuringiensis gène résistance aux organismes nuisibles Diatraea grandiosella Spodoptera frugiperda Diatraea saccharalis http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_30309 http://aims.fao.org/aos/agrovoc/c_25452 http://aims.fao.org/aos/agrovoc/c_30310 F30 - Génétique et amélioration des plantes Zea mays plante transgénique Bacillus thuringiensis gène résistance aux organismes nuisibles Diatraea grandiosella Spodoptera frugiperda Diatraea saccharalis http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_30309 http://aims.fao.org/aos/agrovoc/c_25452 http://aims.fao.org/aos/agrovoc/c_30310 Bohorova, N. Frutos, Roger Royer, Monique Estanol, Pilar Pacheco, M. Rascon, Q. McLean, S. Hoisington, David Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize |
description |
In order to develop a resistance management strategy to control tropical pests based on the co-expression of different toxins, a fully modified Bacillus thuringiensis cry1B gene and the translational fusion cry1B-cry1Ab gene have been developed. Both constructs were cloned under the control of a maize ubiquitin-1 or a rice actin-1 promoter and linked to the bar gene driven by the CaMV 35S promoter. Immature embryos from the tropical lines CML72, CML216, and their hybrids, were used as the target for transformation by microprojectile bombardment. Twenty five percent of the transformed maize plants with cry1B expressed a protein that is active against southwestern corn borer and sugarcane borer. Ten percent of the transgenic maize expressed single fusion proteins from the translational fusion gene cry1B-1Ab and showed resistance to these two pests as well as to the fall armyworm. Transgenic maize plants that carried the cry1B gene in T1 to T3 progenies transmitted trangenes with expected Mendelian segregation and conferred resistance to the two target insects. Molecular analyses confirmed the cry genes integration, the copy number, the size of protein(s) expressed in maize plants, the transmission, and the inheritance of the introduced cry gene. These new transgenic products will provide another recourse for reducing the build-up of resistance in pest populations. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Zea mays plante transgénique Bacillus thuringiensis gène résistance aux organismes nuisibles Diatraea grandiosella Spodoptera frugiperda Diatraea saccharalis http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_30309 http://aims.fao.org/aos/agrovoc/c_25452 http://aims.fao.org/aos/agrovoc/c_30310 |
author |
Bohorova, N. Frutos, Roger Royer, Monique Estanol, Pilar Pacheco, M. Rascon, Q. McLean, S. Hoisington, David |
author_facet |
Bohorova, N. Frutos, Roger Royer, Monique Estanol, Pilar Pacheco, M. Rascon, Q. McLean, S. Hoisington, David |
author_sort |
Bohorova, N. |
title |
Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize |
title_short |
Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize |
title_full |
Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize |
title_fullStr |
Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize |
title_full_unstemmed |
Novel synthetic Bacillus thuringiensis cry1B gene and the cry1B-cry1Ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize |
title_sort |
novel synthetic bacillus thuringiensis cry1b gene and the cry1b-cry1ab translational fusion confer resistance to southwestern corn borer, sugarcane borer and fall armyworm in transgenic tropical maize |
url |
http://agritrop.cirad.fr/487164/ http://agritrop.cirad.fr/487164/1/487164.pdf |
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