Evaluation of genetic relationships in Citrus genus by means of sequence tagged microsatellites

Simple Sequence Repeats (SSRs) have proven to be useful genetic markers in a wide variety of plants, but have not yet to be widely applied to #Citrus# identification. In this study, genetic relationships among 73 individuals of 8 more economically important species of the #Citrus# genus (#C. reticulata# Blanco, #C. sinensis# (L.) Osb., #C. aurantium# L., #C. paradisi# Macf., #C. maxima# (Burm.) Merr., #C. limon# (L.) Burm., #C. aurantifolia# (Christm,) Swing. and #C. medica# L.) were estimated by the amplification of 10 loci of SSR. The potential of STMS for cultivar identification within species has been evaluated and a neighbour joining analysis on the basis of a dissimilarity matrix among the different genotypes has been performed. Polymorphism of 8 STMS was sufficient so as to obtain genetic aggregations where three main groups were clearly identified: the orangemandarin group, the pummelo-grapefruit group and the lime-citron-lemon group. These #Citrus# species relationships were in large agreement with those established by previous analyses based on molecular markers and morphological traits, suggesting that SSR data will be useful for exploring infra-generic #Citrus# genetic taxonomy. The studied microsatellites have not enabled varieties within some of the species, known to have evolved by somatic mutations to be distinguished, such as sweet oranges, grapefruits and sour oranges. However polymorphism has been detected among lemon varieties.

Bibliographic Details

Main Authors:	Luro, François, Rist, Delphine, Ollitrault, Patrick
Format:	conference_item biblioteca
Language:	eng
Published:	ISHS
Subjects:	F30 - Génétique et amélioration des plantes, Citrus, ressource génétique, distance génétique, gène, microsatellite, marqueur génétique, taxonomie, identification, génotype, polymorphisme génétique, http://aims.fao.org/aos/agrovoc/c_1637, http://aims.fao.org/aos/agrovoc/c_3218, http://aims.fao.org/aos/agrovoc/c_27530, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_36574, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_7631, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_3225, http://aims.fao.org/aos/agrovoc/c_24031,
Online Access:	http://agritrop.cirad.fr/485551/
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