AFLP variation within Passiflora subgenus Tacsonia and between Tacsonia and other subgenera

DNA variation was studied in 35 accessions representing 14 species of Passiflora subgenus Tacsonia (including five cultivated species), P. manicata (subg. Manicata), four species of subgenus Passiflora, P. trinervia (subg. Psylanthus), and P. alnifolia (subg. Decaloba). Two primer combinations were retained out of 24 tested. They generated 34 to 80 scorable bands per genotype, for a total of 260 bands on the whole sample. The five wild accessions of P. mixta showed higher polymorphism than those of the cultivated P. tripartita var. mollissima, P. sp'curuba india', and P. pinnatistipula. DNA fingerprints of P. x rosea clearly correspond to the superposition of the bands from its putative parents, P. tripartita var. mollissima and P. pinnatistipula. Cluster analysis is consistent with taxonomical classification into subgenera, with P. manicata intermediate between subgenera Tacsonia and Passijlora, but less so concerning Tacsonia sections. Within this subgenus, P. mixta, P. tripartita var. mollissima, P. sp'curuba india', and P. cumbalensis show particular affinity, as compared with P. pinnatistipula and less common species. Distances between Tacsonia and other subgenera appear limîted as compared to distances within Tacsonia, suggesting the use of other techniques to study the relationships between subgenera.

Bibliographic Details

Main Authors:	Segura, S.D., Coppens D'Eeckenbrugge, Géo, Ollitrault, Patrick, Bohorquez, Adriana, Tohmé, Joe
Format:	conference_item biblioteca
Language:	eng
Published:	s.n.
Subjects:	F30 - Génétique et amélioration des plantes,
Online Access:	http://agritrop.cirad.fr/477499/
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