The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice
Abstract Almost all the nuclear genes of four Gramineae (maize, wheat, barley, rice) and pea are located in DNA fractions covering only a 1-2% GC range and representing between 10 and 25% of the different genomes. These DNA fractions comprise large gene-rich regions (collectively called the 'gene space') separated by vast gene-empty, repeated sequences. In contrast, in Arabidopsis thaliana, genes are distributed in DNA fractions covering an 8% GC range and representing 85% of the genome. Here, we investigated the integration of a transferred DNA (T-DNA) in the genomes of Arabidopsis and rice and found different patterns of integration, which are correlated with the different gene distributions. While T-DNA integrates essentially everywhere in the Arabidopsis genome, integration was detected only in the gene space, namely in the gene-rich, transcriptionally active, regions of the rice genome. The implications of these results for the integration of foreign DNA are discussed.
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Subjects: | F30 - Génétique et amélioration des plantes, Arabidopsis, Oryza sativa, plante transgénique, transfert de gène, localisation de gène, http://aims.fao.org/aos/agrovoc/c_33291, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_27619, http://aims.fao.org/aos/agrovoc/c_24846, http://aims.fao.org/aos/agrovoc/c_24845, |
Online Access: | http://agritrop.cirad.fr/476847/ http://agritrop.cirad.fr/476847/1/476847.pdf |
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dig-cirad-fr-4768472024-01-28T08:40:03Z http://agritrop.cirad.fr/476847/ http://agritrop.cirad.fr/476847/ The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice. Barakat Abdelali, Gallois Patrick, Raynal Martine, Mestre-Ortega Dominique, Sallaud Christophe, Guiderdoni Emmanuel, Delseny Michel, Bernardi Giorgio. 2000. FEBS Letters (471) : 161-164.https://doi.org/10.1016/S0014-5793(00)01393-4 <https://doi.org/10.1016/S0014-5793(00)01393-4> The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice Barakat, Abdelali Gallois, Patrick Raynal, Martine Mestre-Ortega, Dominique Sallaud, Christophe Guiderdoni, Emmanuel Delseny, Michel Bernardi, Giorgio eng 2000 FEBS Letters F30 - Génétique et amélioration des plantes Arabidopsis Oryza sativa plante transgénique transfert de gène localisation de gène http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_24845 Abstract Almost all the nuclear genes of four Gramineae (maize, wheat, barley, rice) and pea are located in DNA fractions covering only a 1-2% GC range and representing between 10 and 25% of the different genomes. These DNA fractions comprise large gene-rich regions (collectively called the 'gene space') separated by vast gene-empty, repeated sequences. In contrast, in Arabidopsis thaliana, genes are distributed in DNA fractions covering an 8% GC range and representing 85% of the genome. Here, we investigated the integration of a transferred DNA (T-DNA) in the genomes of Arabidopsis and rice and found different patterns of integration, which are correlated with the different gene distributions. While T-DNA integrates essentially everywhere in the Arabidopsis genome, integration was detected only in the gene space, namely in the gene-rich, transcriptionally active, regions of the rice genome. The implications of these results for the integration of foreign DNA are discussed. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/476847/1/476847.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/S0014-5793(00)01393-4 10.1016/S0014-5793(00)01393-4 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=162508 info:eu-repo/semantics/altIdentifier/doi/10.1016/S0014-5793(00)01393-4 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/S0014-5793(00)01393-4 |
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F30 - Génétique et amélioration des plantes Arabidopsis Oryza sativa plante transgénique transfert de gène localisation de gène http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_24845 F30 - Génétique et amélioration des plantes Arabidopsis Oryza sativa plante transgénique transfert de gène localisation de gène http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_24845 |
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F30 - Génétique et amélioration des plantes Arabidopsis Oryza sativa plante transgénique transfert de gène localisation de gène http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_24845 F30 - Génétique et amélioration des plantes Arabidopsis Oryza sativa plante transgénique transfert de gène localisation de gène http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_24845 Barakat, Abdelali Gallois, Patrick Raynal, Martine Mestre-Ortega, Dominique Sallaud, Christophe Guiderdoni, Emmanuel Delseny, Michel Bernardi, Giorgio The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice |
description |
Abstract Almost all the nuclear genes of four Gramineae (maize, wheat, barley, rice) and pea are located in DNA fractions covering only a 1-2% GC range and representing between 10 and 25% of the different genomes. These DNA fractions comprise large gene-rich regions (collectively called the 'gene space') separated by vast gene-empty, repeated sequences. In contrast, in Arabidopsis thaliana, genes are distributed in DNA fractions covering an 8% GC range and representing 85% of the genome. Here, we investigated the integration of a transferred DNA (T-DNA) in the genomes of Arabidopsis and rice and found different patterns of integration, which are correlated with the different gene distributions. While T-DNA integrates essentially everywhere in the Arabidopsis genome, integration was detected only in the gene space, namely in the gene-rich, transcriptionally active, regions of the rice genome. The implications of these results for the integration of foreign DNA are discussed. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Arabidopsis Oryza sativa plante transgénique transfert de gène localisation de gène http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_27619 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_24845 |
author |
Barakat, Abdelali Gallois, Patrick Raynal, Martine Mestre-Ortega, Dominique Sallaud, Christophe Guiderdoni, Emmanuel Delseny, Michel Bernardi, Giorgio |
author_facet |
Barakat, Abdelali Gallois, Patrick Raynal, Martine Mestre-Ortega, Dominique Sallaud, Christophe Guiderdoni, Emmanuel Delseny, Michel Bernardi, Giorgio |
author_sort |
Barakat, Abdelali |
title |
The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice |
title_short |
The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice |
title_full |
The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice |
title_fullStr |
The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice |
title_full_unstemmed |
The distribution of T-DNA in the genomes of transgenic Arabidopsis and rice |
title_sort |
distribution of t-dna in the genomes of transgenic arabidopsis and rice |
url |
http://agritrop.cirad.fr/476847/ http://agritrop.cirad.fr/476847/1/476847.pdf |
work_keys_str_mv |
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