Cloning and expression of genes involved in oxidative stress in the latex from TPD trees
Tapping Panel Dryness (TPD) is a disease of rubber tree characterized by the reduction or total cessation of latex flow upon tapping, due to senescence of the laticifers leading to abnormal coagulation processes. The hypothesis of an oxidative stress-responsible for the damaging of the lutoid membrane is considered. Northern blot analysis showed that moderate ethylene treatment stimulated catalase, Mn-superoxide dismutase gene expression. However, overstimulation-indu ced TPD trees do not respond to ethylene, both in views of catalase and Mn-superoxide dismutase mRNA level and catalase activity. The overall results led to conclude that the modification of expression of catalase and Mn-superoxide dismutase genes in sick and healthy trees may be involved in the onset of overstimulation-induced TPD. Oxidative stress may not be involved in spontaneous TPD as no difference was observed in terms of catalase and Mn-superoxide dismutase transcripts level between sick and healthy trees. A full-length catalase cDNA from rubber tree latex was cloned and sequenced. It was found that the catalase cDNA consisted of1,476 nucleotides which encoded for a protein of 492 amino acids with molecular weight 56.7 kDa. Sequence comparison showed that this rubber tree catalase cDNA was highly homologous to several other plant catalases, in which the highest score of homology was the #R. communis#. catalase gene CAT1, with 89 and 91 % homology in nucleotides and amino acids, respectivel.
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Mahidol University
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Subjects: | F30 - Génétique et amélioration des plantes, Hevea brasiliensis, latex, expression des gènes, clonage, stress, oxydation, incision, superoxyde dismutase, éthylène, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_4214, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_15952, http://aims.fao.org/aos/agrovoc/c_7452, http://aims.fao.org/aos/agrovoc/c_5472, http://aims.fao.org/aos/agrovoc/c_3819, http://aims.fao.org/aos/agrovoc/c_24091, http://aims.fao.org/aos/agrovoc/c_2679, |
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dig-cirad-fr-4649912024-01-28T07:03:43Z http://agritrop.cirad.fr/464991/ http://agritrop.cirad.fr/464991/ Cloning and expression of genes involved in oxidative stress in the latex from TPD trees. Kongsawadworakul Panida, Pujade-Renaud Valérie, Chrestin Hervé, Montoro Pascal, Lacrotte Régis, Narangajavana J.. 1997. In : Seminar on the biochemical and molecular tools for exploitation diagnostic and rubber tree improvement. Workshop on electrophoresis application to rubber tree clone identification. Mahidol University, RRIT, ORSTOM, CIRAD. Bangkok : Mahidol University, 9 p. Seminar on the Biochemical and Molecular Tools for Exploitation Diagnostic and Rubber Tree Improvement, Bangkok, Thaïlande, 20 Octobre 1997/22 Octobre 1997. Cloning and expression of genes involved in oxidative stress in the latex from TPD trees Kongsawadworakul, Panida Pujade-Renaud, Valérie Chrestin, Hervé Montoro, Pascal Lacrotte, Régis Narangajavana, J. eng 1997 Mahidol University Seminar on the biochemical and molecular tools for exploitation diagnostic and rubber tree improvement. Workshop on electrophoresis application to rubber tree clone identification F30 - Génétique et amélioration des plantes Hevea brasiliensis latex expression des gènes clonage stress oxydation incision superoxyde dismutase éthylène http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_15952 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_3819 http://aims.fao.org/aos/agrovoc/c_24091 http://aims.fao.org/aos/agrovoc/c_2679 Tapping Panel Dryness (TPD) is a disease of rubber tree characterized by the reduction or total cessation of latex flow upon tapping, due to senescence of the laticifers leading to abnormal coagulation processes. The hypothesis of an oxidative stress-responsible for the damaging of the lutoid membrane is considered. Northern blot analysis showed that moderate ethylene treatment stimulated catalase, Mn-superoxide dismutase gene expression. However, overstimulation-indu ced TPD trees do not respond to ethylene, both in views of catalase and Mn-superoxide dismutase mRNA level and catalase activity. The overall results led to conclude that the modification of expression of catalase and Mn-superoxide dismutase genes in sick and healthy trees may be involved in the onset of overstimulation-induced TPD. Oxidative stress may not be involved in spontaneous TPD as no difference was observed in terms of catalase and Mn-superoxide dismutase transcripts level between sick and healthy trees. A full-length catalase cDNA from rubber tree latex was cloned and sequenced. It was found that the catalase cDNA consisted of1,476 nucleotides which encoded for a protein of 492 amino acids with molecular weight 56.7 kDa. Sequence comparison showed that this rubber tree catalase cDNA was highly homologous to several other plant catalases, in which the highest score of homology was the #R. communis#. catalase gene CAT1, with 89 and 91 % homology in nucleotides and amino acids, respectivel. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/464991/1/ID464991.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html http://agritrop.cirad.fr/314321/ |
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F30 - Génétique et amélioration des plantes Hevea brasiliensis latex expression des gènes clonage stress oxydation incision superoxyde dismutase éthylène http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_15952 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_3819 http://aims.fao.org/aos/agrovoc/c_24091 http://aims.fao.org/aos/agrovoc/c_2679 F30 - Génétique et amélioration des plantes Hevea brasiliensis latex expression des gènes clonage stress oxydation incision superoxyde dismutase éthylène http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_15952 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_3819 http://aims.fao.org/aos/agrovoc/c_24091 http://aims.fao.org/aos/agrovoc/c_2679 |
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F30 - Génétique et amélioration des plantes Hevea brasiliensis latex expression des gènes clonage stress oxydation incision superoxyde dismutase éthylène http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_15952 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_3819 http://aims.fao.org/aos/agrovoc/c_24091 http://aims.fao.org/aos/agrovoc/c_2679 F30 - Génétique et amélioration des plantes Hevea brasiliensis latex expression des gènes clonage stress oxydation incision superoxyde dismutase éthylène http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_15952 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_3819 http://aims.fao.org/aos/agrovoc/c_24091 http://aims.fao.org/aos/agrovoc/c_2679 Kongsawadworakul, Panida Pujade-Renaud, Valérie Chrestin, Hervé Montoro, Pascal Lacrotte, Régis Narangajavana, J. Cloning and expression of genes involved in oxidative stress in the latex from TPD trees |
description |
Tapping Panel Dryness (TPD) is a disease of rubber tree characterized by the reduction or total cessation of latex flow upon tapping, due to senescence of the laticifers leading to abnormal coagulation processes. The hypothesis of an oxidative stress-responsible for the damaging of the lutoid membrane is considered. Northern blot analysis showed that moderate ethylene treatment stimulated catalase, Mn-superoxide dismutase gene expression. However, overstimulation-indu ced TPD trees do not respond to ethylene, both in views of catalase and Mn-superoxide dismutase mRNA level and catalase activity. The overall results led to conclude that the modification of expression of catalase and Mn-superoxide dismutase genes in sick and healthy trees may be involved in the onset of overstimulation-induced TPD. Oxidative stress may not be involved in spontaneous TPD as no difference was observed in terms of catalase and Mn-superoxide dismutase transcripts level between sick and healthy trees. A full-length catalase cDNA from rubber tree latex was cloned and sequenced. It was found that the catalase cDNA consisted of1,476 nucleotides which encoded for a protein of 492 amino acids with molecular weight 56.7 kDa. Sequence comparison showed that this rubber tree catalase cDNA was highly homologous to several other plant catalases, in which the highest score of homology was the #R. communis#. catalase gene CAT1, with 89 and 91 % homology in nucleotides and amino acids, respectivel. |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes Hevea brasiliensis latex expression des gènes clonage stress oxydation incision superoxyde dismutase éthylène http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_15952 http://aims.fao.org/aos/agrovoc/c_7452 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_3819 http://aims.fao.org/aos/agrovoc/c_24091 http://aims.fao.org/aos/agrovoc/c_2679 |
author |
Kongsawadworakul, Panida Pujade-Renaud, Valérie Chrestin, Hervé Montoro, Pascal Lacrotte, Régis Narangajavana, J. |
author_facet |
Kongsawadworakul, Panida Pujade-Renaud, Valérie Chrestin, Hervé Montoro, Pascal Lacrotte, Régis Narangajavana, J. |
author_sort |
Kongsawadworakul, Panida |
title |
Cloning and expression of genes involved in oxidative stress in the latex from TPD trees |
title_short |
Cloning and expression of genes involved in oxidative stress in the latex from TPD trees |
title_full |
Cloning and expression of genes involved in oxidative stress in the latex from TPD trees |
title_fullStr |
Cloning and expression of genes involved in oxidative stress in the latex from TPD trees |
title_full_unstemmed |
Cloning and expression of genes involved in oxidative stress in the latex from TPD trees |
title_sort |
cloning and expression of genes involved in oxidative stress in the latex from tpd trees |
publisher |
Mahidol University |
url |
http://agritrop.cirad.fr/464991/ http://agritrop.cirad.fr/464991/1/ID464991.pdf |
work_keys_str_mv |
AT kongsawadworakulpanida cloningandexpressionofgenesinvolvedinoxidativestressinthelatexfromtpdtrees AT pujaderenaudvalerie cloningandexpressionofgenesinvolvedinoxidativestressinthelatexfromtpdtrees AT chrestinherve cloningandexpressionofgenesinvolvedinoxidativestressinthelatexfromtpdtrees AT montoropascal cloningandexpressionofgenesinvolvedinoxidativestressinthelatexfromtpdtrees AT lacrotteregis cloningandexpressionofgenesinvolvedinoxidativestressinthelatexfromtpdtrees AT narangajavanaj cloningandexpressionofgenesinvolvedinoxidativestressinthelatexfromtpdtrees |
_version_ |
1792494310946504704 |