Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara)

More than 750 plants were regenerated f rom protoplasts isolated from microspore callus-derived cell suspensions of the Mediterranean japonica rice Miara, using a nurse-feeder technique and N6-based culture medium. The mean plating efficiency and the mean regeneration ability of the protocalluses were 0.5% and 49% respectively. Flow cytometric evaluation of the DNA contents of 7 month old-cell and protoplast suspensions showed that they were still haploid. Contrastingly, the DNA contents of leaf cell nuclei of the regenerated protoclones ranged from 1C to 5C including 60% 2C plants. This was consistent with the morphological type and the fertility of the mature plants. These results and the absence of chimeric plants suggest that polyploidization occurred during the early phase of protoplast culture.

Bibliographic Details

Main Authors:	Guiderdoni, Emmanuel, Chaïr, Hâna
Format:	article biblioteca
Language:	eng
Subjects:	F30 - Génétique et amélioration des plantes, Oryza sativa, protoplaste, culture de cellule, haploïdie, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_13629, http://aims.fao.org/aos/agrovoc/c_7178, http://aims.fao.org/aos/agrovoc/c_3490,
Online Access:	http://agritrop.cirad.fr/419275/ http://agritrop.cirad.fr/419275/1/419275.pdf
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