Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara)
More than 750 plants were regenerated f rom protoplasts isolated from microspore callus-derived cell suspensions of the Mediterranean japonica rice Miara, using a nurse-feeder technique and N6-based culture medium. The mean plating efficiency and the mean regeneration ability of the protocalluses were 0.5% and 49% respectively. Flow cytometric evaluation of the DNA contents of 7 month old-cell and protoplast suspensions showed that they were still haploid. Contrastingly, the DNA contents of leaf cell nuclei of the regenerated protoclones ranged from 1C to 5C including 60% 2C plants. This was consistent with the morphological type and the fertility of the mature plants. These results and the absence of chimeric plants suggest that polyploidization occurred during the early phase of protoplast culture.
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Subjects: | F30 - Génétique et amélioration des plantes, Oryza sativa, protoplaste, culture de cellule, haploïdie, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_13629, http://aims.fao.org/aos/agrovoc/c_7178, http://aims.fao.org/aos/agrovoc/c_3490, |
Online Access: | http://agritrop.cirad.fr/419275/ http://agritrop.cirad.fr/419275/1/419275.pdf |
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dig-cirad-fr-4192752024-01-28T02:44:40Z http://agritrop.cirad.fr/419275/ http://agritrop.cirad.fr/419275/ Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara). Guiderdoni Emmanuel, Chaïr Hâna. 1992. Plant Cell Reports, 11 (12) : 618-622.https://doi.org/10.1007/BF00236385 <https://doi.org/10.1007/BF00236385> Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara) Guiderdoni, Emmanuel Chaïr, Hâna eng 1992 Plant Cell Reports F30 - Génétique et amélioration des plantes Oryza sativa protoplaste culture de cellule haploïdie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_7178 http://aims.fao.org/aos/agrovoc/c_3490 More than 750 plants were regenerated f rom protoplasts isolated from microspore callus-derived cell suspensions of the Mediterranean japonica rice Miara, using a nurse-feeder technique and N6-based culture medium. The mean plating efficiency and the mean regeneration ability of the protocalluses were 0.5% and 49% respectively. Flow cytometric evaluation of the DNA contents of 7 month old-cell and protoplast suspensions showed that they were still haploid. Contrastingly, the DNA contents of leaf cell nuclei of the regenerated protoclones ranged from 1C to 5C including 60% 2C plants. This was consistent with the morphological type and the fertility of the mature plants. These results and the absence of chimeric plants suggest that polyploidization occurred during the early phase of protoplast culture. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/419275/1/419275.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/BF00236385 10.1007/BF00236385 info:eu-repo/semantics/altIdentifier/doi/10.1007/BF00236385 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/BF00236385 |
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F30 - Génétique et amélioration des plantes Oryza sativa protoplaste culture de cellule haploïdie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_7178 http://aims.fao.org/aos/agrovoc/c_3490 F30 - Génétique et amélioration des plantes Oryza sativa protoplaste culture de cellule haploïdie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_7178 http://aims.fao.org/aos/agrovoc/c_3490 |
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F30 - Génétique et amélioration des plantes Oryza sativa protoplaste culture de cellule haploïdie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_7178 http://aims.fao.org/aos/agrovoc/c_3490 F30 - Génétique et amélioration des plantes Oryza sativa protoplaste culture de cellule haploïdie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_7178 http://aims.fao.org/aos/agrovoc/c_3490 Guiderdoni, Emmanuel Chaïr, Hâna Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara) |
description |
More than 750 plants were regenerated f rom protoplasts isolated from microspore callus-derived cell suspensions of the Mediterranean japonica rice Miara, using a nurse-feeder technique and N6-based culture medium. The mean plating efficiency and the mean regeneration ability of the protocalluses were 0.5% and 49% respectively. Flow cytometric evaluation of the DNA contents of 7 month old-cell and protoplast suspensions showed that they were still haploid. Contrastingly, the DNA contents of leaf cell nuclei of the regenerated protoclones ranged from 1C to 5C including 60% 2C plants. This was consistent with the morphological type and the fertility of the mature plants. These results and the absence of chimeric plants suggest that polyploidization occurred during the early phase of protoplast culture. |
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F30 - Génétique et amélioration des plantes Oryza sativa protoplaste culture de cellule haploïdie http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_7178 http://aims.fao.org/aos/agrovoc/c_3490 |
author |
Guiderdoni, Emmanuel Chaïr, Hâna |
author_facet |
Guiderdoni, Emmanuel Chaïr, Hâna |
author_sort |
Guiderdoni, Emmanuel |
title |
Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara) |
title_short |
Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara) |
title_full |
Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara) |
title_fullStr |
Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara) |
title_full_unstemmed |
Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara) |
title_sort |
plant regeneration from hybrid cell suspension derived protoplasts of mediterranean rice (oryza sativa l. cv. miara) |
url |
http://agritrop.cirad.fr/419275/ http://agritrop.cirad.fr/419275/1/419275.pdf |
work_keys_str_mv |
AT guiderdoniemmanuel plantregenerationfromhybridcellsuspensionderivedprotoplastsofmediterraneanriceoryzasativalcvmiara AT chairhana plantregenerationfromhybridcellsuspensionderivedprotoplastsofmediterraneanriceoryzasativalcvmiara |
_version_ |
1792492660876902400 |