Susceptibility of the coffee leaf miner (Perileucoptera sp.) to Bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects
Binding of several Bacillus thuringiensis delta-endotoxins was studied on histological midgut sections of larvae of coffee leaf miner Perileucoptera coffeella from Brazil and Perileucoptera sp from Madagascar. CryIA(a), CryIA(b), CryIA(c), CryIB, CryIE, and CryllA were tested for binding, and only CrylA(c), CrylB, and CrylE yielded a positive response. The toxins bound to the whole midgut, and the result was identical on both insect populations. The same toxins, to the number of which CrylC was added, were tested on larvae of P. coffeella. CrylA(c) and CrylB were toxic with an LC50 of 1.47 pg/ml and 21.93 pg/ml, respectively. CrylE was not toxic to P. coffeella. CrylA(c) and CrylB were tested for synergistic activity and were shown to act by cumulative effect when delivered to the insect larvae as a mixture.
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Subjects: | H10 - Ravageurs des plantes, Perileucoptera, endotoxine, toxine, Bacillus thuringiensis, http://aims.fao.org/aos/agrovoc/c_30238, http://aims.fao.org/aos/agrovoc/c_2564, http://aims.fao.org/aos/agrovoc/c_7828, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_1070, http://aims.fao.org/aos/agrovoc/c_4510, |
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dig-cirad-fr-4012002024-01-28T01:13:17Z http://agritrop.cirad.fr/401200/ http://agritrop.cirad.fr/401200/ Susceptibility of the coffee leaf miner (Perileucoptera sp.) to Bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects. Guerreiro Filho Oliveiro, Denolf P., Peferoen M., Decazy Bernard, Eskes Albertus, Frutos Roger. 1998. Current Microbiology, 36 : 175-179.https://doi.org/10.1007/PL00006763 <https://doi.org/10.1007/PL00006763> Susceptibility of the coffee leaf miner (Perileucoptera sp.) to Bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects Guerreiro Filho, Oliveiro Denolf, P. Peferoen, M. Decazy, Bernard Eskes, Albertus Frutos, Roger eng 1998 Current Microbiology H10 - Ravageurs des plantes Perileucoptera endotoxine toxine Bacillus thuringiensis http://aims.fao.org/aos/agrovoc/c_30238 http://aims.fao.org/aos/agrovoc/c_2564 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_761 Brésil Madagascar http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4510 Binding of several Bacillus thuringiensis delta-endotoxins was studied on histological midgut sections of larvae of coffee leaf miner Perileucoptera coffeella from Brazil and Perileucoptera sp from Madagascar. CryIA(a), CryIA(b), CryIA(c), CryIB, CryIE, and CryllA were tested for binding, and only CrylA(c), CrylB, and CrylE yielded a positive response. The toxins bound to the whole midgut, and the result was identical on both insect populations. The same toxins, to the number of which CrylC was added, were tested on larvae of P. coffeella. CrylA(c) and CrylB were toxic with an LC50 of 1.47 pg/ml and 21.93 pg/ml, respectively. CrylE was not toxic to P. coffeella. CrylA(c) and CrylB were tested for synergistic activity and were shown to act by cumulative effect when delivered to the insect larvae as a mixture. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/401200/1/401200.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/PL00006763 10.1007/PL00006763 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=98394 info:eu-repo/semantics/altIdentifier/doi/10.1007/PL00006763 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/PL00006763 |
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H10 - Ravageurs des plantes Perileucoptera endotoxine toxine Bacillus thuringiensis http://aims.fao.org/aos/agrovoc/c_30238 http://aims.fao.org/aos/agrovoc/c_2564 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4510 H10 - Ravageurs des plantes Perileucoptera endotoxine toxine Bacillus thuringiensis http://aims.fao.org/aos/agrovoc/c_30238 http://aims.fao.org/aos/agrovoc/c_2564 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4510 |
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H10 - Ravageurs des plantes Perileucoptera endotoxine toxine Bacillus thuringiensis http://aims.fao.org/aos/agrovoc/c_30238 http://aims.fao.org/aos/agrovoc/c_2564 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4510 H10 - Ravageurs des plantes Perileucoptera endotoxine toxine Bacillus thuringiensis http://aims.fao.org/aos/agrovoc/c_30238 http://aims.fao.org/aos/agrovoc/c_2564 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4510 Guerreiro Filho, Oliveiro Denolf, P. Peferoen, M. Decazy, Bernard Eskes, Albertus Frutos, Roger Susceptibility of the coffee leaf miner (Perileucoptera sp.) to Bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects |
description |
Binding of several Bacillus thuringiensis delta-endotoxins was studied on histological midgut sections of larvae of coffee leaf miner Perileucoptera coffeella from Brazil and Perileucoptera sp from Madagascar. CryIA(a), CryIA(b), CryIA(c), CryIB, CryIE, and CryllA were tested for binding, and only CrylA(c), CrylB, and CrylE yielded a positive response. The toxins bound to the whole midgut, and the result was identical on both insect populations. The same toxins, to the number of which CrylC was added, were tested on larvae of P. coffeella. CrylA(c) and CrylB were toxic with an LC50 of 1.47 pg/ml and 21.93 pg/ml, respectively. CrylE was not toxic to P. coffeella. CrylA(c) and CrylB were tested for synergistic activity and were shown to act by cumulative effect when delivered to the insect larvae as a mixture. |
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article |
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H10 - Ravageurs des plantes Perileucoptera endotoxine toxine Bacillus thuringiensis http://aims.fao.org/aos/agrovoc/c_30238 http://aims.fao.org/aos/agrovoc/c_2564 http://aims.fao.org/aos/agrovoc/c_7828 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4510 |
author |
Guerreiro Filho, Oliveiro Denolf, P. Peferoen, M. Decazy, Bernard Eskes, Albertus Frutos, Roger |
author_facet |
Guerreiro Filho, Oliveiro Denolf, P. Peferoen, M. Decazy, Bernard Eskes, Albertus Frutos, Roger |
author_sort |
Guerreiro Filho, Oliveiro |
title |
Susceptibility of the coffee leaf miner (Perileucoptera sp.) to Bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects |
title_short |
Susceptibility of the coffee leaf miner (Perileucoptera sp.) to Bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects |
title_full |
Susceptibility of the coffee leaf miner (Perileucoptera sp.) to Bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects |
title_fullStr |
Susceptibility of the coffee leaf miner (Perileucoptera sp.) to Bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects |
title_full_unstemmed |
Susceptibility of the coffee leaf miner (Perileucoptera sp.) to Bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects |
title_sort |
susceptibility of the coffee leaf miner (perileucoptera sp.) to bacillus thuringiensis delta-endotoxins : a model for transgenic perennial crops resistant to endocarpic insects |
url |
http://agritrop.cirad.fr/401200/ http://agritrop.cirad.fr/401200/1/401200.pdf |
work_keys_str_mv |
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