In vitro cultivation of Phytomonas from latex and phloem-restricte d Phytomonas

The #in vitro# culture of #Phytomonas# was the gain obstacle to their study. In and after 1982 we were able to grow #Phytomonas# from laticiferous plants: #Euphorbia pinea#, #E. hirta#, #E. hyssopifolia#, #Mandevilla scabra#, from different areas of the world. But phloem-restricted #Phytomonas# remained difficult to grow and it was not until 1986 we obtained the first primoculture of #Phytomonas# associated with Hartrot disease of coconut, in a medium containing insect cells. After several passages axenic cultures have been obtained. Today we are growing 9 isolates of Hartrot of French Guiana, one from Brazil and two from Venezuela. #Phytomonas# associated with Marchitez disease of oil palm were cultured for the first time in 1989. Four isolates from Colombia are now being cultured. Four of these phloem-restricted isolates have been cloned (37 clones). Recently #Phytomonas# (apparently phloem-restricted) were discovered in Grenada by P. HUNT in #Alpinia purpurata#. Two isolates were obtained in insect cell cultures in the laboratory.

Bibliographic Details

Main Authors:	Gargani, Daniel, Menara, C., Segur, C., Dollet, Michel
Format:	conference_item biblioteca
Language:	eng
Published:	s.n.
Subjects:	H20 - Maladies des plantes, tissu vasculaire, phloème, Phytomonas, culture in vitro, latex, Euphorbia, expérimentation en laboratoire, http://aims.fao.org/aos/agrovoc/c_8161, http://aims.fao.org/aos/agrovoc/c_5787, http://aims.fao.org/aos/agrovoc/c_26618, http://aims.fao.org/aos/agrovoc/c_24135, http://aims.fao.org/aos/agrovoc/c_4214, http://aims.fao.org/aos/agrovoc/c_2717, http://aims.fao.org/aos/agrovoc/c_15989, http://aims.fao.org/aos/agrovoc/c_3081,
Online Access:	http://agritrop.cirad.fr/400820/
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