La maladie de la mosaïque des bractées des bananiers

Two viruses are systematically involved in banana bract mosaic disease, BBrMD: a potyvirus, BBrMV, responsible for the disease and uncharacterized filamentous viral particles identified as potexvirus. The potyvirus was the only one transmitted by Pentalonia nigronervosa to healthy banana plants. It is pathogenic and induces typical symptoms of the disease (Fig. 1).Antisera were developed against it and reacted with one protein of an estimated size of 36kD in western blot. No reaction was obtained with the specific monoclonalpotexvirus, confirming infection by the potyvirus alone (Fig. 2). Because of these observations, this strain is considered the reference strain -"Ref" strain - of BBrMD in the Philippines. Molecular analysis of the CP gene and UTR region was conducted (Fig. 3). Comparisons with other BBrMV isolates (Philippines and India) and with the potyvirus gene data bank showed the "Ref" strain and the BBrMV isolates to be the same virus and the "Ref" strain a different potyvirus from the other potyviruses analysed (Fig. 4). Three couples of primers defined from the "Ref" sequence were used to study the molecular variability of BBrMV in the field. The expected amplified fragments were obtained for 5 differents isolates. No significant variability was observed between the isolates and the "Ref" strain after sequencing. The "Ref" strain was thus representative of BBrMD in the field. The identification of specific primers for molecular detection is in progress. The presence of the potexvirus in BBrMD remains unexplained.

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Bibliographic Details
Main Authors: Caruana, Marie-Line, Galzi, Serge, Laboureau, Nathalie, Bousalem, M., Royer, Monique, Frutos, Roger
Format: book_section biblioteca
Language:fre
Published: CIRAD
Subjects:H20 - Maladies des plantes, Musa, virus des végétaux, Potyvirus, bractée, identification, immunologie, séquence nucléotidique, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_32866, http://aims.fao.org/aos/agrovoc/c_1052, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_3808, http://aims.fao.org/aos/agrovoc/c_27583,
Online Access:http://agritrop.cirad.fr/392292/
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spelling dig-cirad-fr-3922922024-01-28T00:03:01Z http://agritrop.cirad.fr/392292/ http://agritrop.cirad.fr/392292/ La maladie de la mosaïque des bractées des bananiers. Caruana Marie-Line, Galzi Serge, Laboureau Nathalie, Bousalem M., Royer Monique, Frutos Roger. 1999. In : LPRC. Rapport d'activités 1996-1998. Fargette Denis (ed.), Peterschmitt Michel (ed.), Ferrer M. (ed.). CIRAD-LPRC, CIRAD-AMIS. Montpellier : CIRAD, 39-42. La maladie de la mosaïque des bractées des bananiers Caruana, Marie-Line Galzi, Serge Laboureau, Nathalie Bousalem, M. Royer, Monique Frutos, Roger fre 1999 CIRAD LPRC. Rapport d'activités 1996-1998 H20 - Maladies des plantes Musa virus des végétaux Potyvirus bractée identification immunologie séquence nucléotidique http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_32866 http://aims.fao.org/aos/agrovoc/c_1052 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_27583 Two viruses are systematically involved in banana bract mosaic disease, BBrMD: a potyvirus, BBrMV, responsible for the disease and uncharacterized filamentous viral particles identified as potexvirus. The potyvirus was the only one transmitted by Pentalonia nigronervosa to healthy banana plants. It is pathogenic and induces typical symptoms of the disease (Fig. 1).Antisera were developed against it and reacted with one protein of an estimated size of 36kD in western blot. No reaction was obtained with the specific monoclonalpotexvirus, confirming infection by the potyvirus alone (Fig. 2). Because of these observations, this strain is considered the reference strain -"Ref" strain - of BBrMD in the Philippines. Molecular analysis of the CP gene and UTR region was conducted (Fig. 3). Comparisons with other BBrMV isolates (Philippines and India) and with the potyvirus gene data bank showed the "Ref" strain and the BBrMV isolates to be the same virus and the "Ref" strain a different potyvirus from the other potyviruses analysed (Fig. 4). Three couples of primers defined from the "Ref" sequence were used to study the molecular variability of BBrMV in the field. The expected amplified fragments were obtained for 5 differents isolates. No significant variability was observed between the isolates and the "Ref" strain after sequencing. The "Ref" strain was thus representative of BBrMD in the field. The identification of specific primers for molecular detection is in progress. The presence of the potexvirus in BBrMD remains unexplained. book_section info:eu-repo/semantics/report Report info:eu-repo/semantics/closedAccess http://agritrop.cirad.fr/300654/
institution CIRAD FR
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-cirad-fr
tag biblioteca
region Europa del Oeste
libraryname Biblioteca del CIRAD Francia
language fre
topic H20 - Maladies des plantes
Musa
virus des végétaux
Potyvirus
bractée
identification
immunologie
séquence nucléotidique
http://aims.fao.org/aos/agrovoc/c_4993
http://aims.fao.org/aos/agrovoc/c_5985
http://aims.fao.org/aos/agrovoc/c_32866
http://aims.fao.org/aos/agrovoc/c_1052
http://aims.fao.org/aos/agrovoc/c_3791
http://aims.fao.org/aos/agrovoc/c_3808
http://aims.fao.org/aos/agrovoc/c_27583
H20 - Maladies des plantes
Musa
virus des végétaux
Potyvirus
bractée
identification
immunologie
séquence nucléotidique
http://aims.fao.org/aos/agrovoc/c_4993
http://aims.fao.org/aos/agrovoc/c_5985
http://aims.fao.org/aos/agrovoc/c_32866
http://aims.fao.org/aos/agrovoc/c_1052
http://aims.fao.org/aos/agrovoc/c_3791
http://aims.fao.org/aos/agrovoc/c_3808
http://aims.fao.org/aos/agrovoc/c_27583
spellingShingle H20 - Maladies des plantes
Musa
virus des végétaux
Potyvirus
bractée
identification
immunologie
séquence nucléotidique
http://aims.fao.org/aos/agrovoc/c_4993
http://aims.fao.org/aos/agrovoc/c_5985
http://aims.fao.org/aos/agrovoc/c_32866
http://aims.fao.org/aos/agrovoc/c_1052
http://aims.fao.org/aos/agrovoc/c_3791
http://aims.fao.org/aos/agrovoc/c_3808
http://aims.fao.org/aos/agrovoc/c_27583
H20 - Maladies des plantes
Musa
virus des végétaux
Potyvirus
bractée
identification
immunologie
séquence nucléotidique
http://aims.fao.org/aos/agrovoc/c_4993
http://aims.fao.org/aos/agrovoc/c_5985
http://aims.fao.org/aos/agrovoc/c_32866
http://aims.fao.org/aos/agrovoc/c_1052
http://aims.fao.org/aos/agrovoc/c_3791
http://aims.fao.org/aos/agrovoc/c_3808
http://aims.fao.org/aos/agrovoc/c_27583
Caruana, Marie-Line
Galzi, Serge
Laboureau, Nathalie
Bousalem, M.
Royer, Monique
Frutos, Roger
La maladie de la mosaïque des bractées des bananiers
description Two viruses are systematically involved in banana bract mosaic disease, BBrMD: a potyvirus, BBrMV, responsible for the disease and uncharacterized filamentous viral particles identified as potexvirus. The potyvirus was the only one transmitted by Pentalonia nigronervosa to healthy banana plants. It is pathogenic and induces typical symptoms of the disease (Fig. 1).Antisera were developed against it and reacted with one protein of an estimated size of 36kD in western blot. No reaction was obtained with the specific monoclonalpotexvirus, confirming infection by the potyvirus alone (Fig. 2). Because of these observations, this strain is considered the reference strain -"Ref" strain - of BBrMD in the Philippines. Molecular analysis of the CP gene and UTR region was conducted (Fig. 3). Comparisons with other BBrMV isolates (Philippines and India) and with the potyvirus gene data bank showed the "Ref" strain and the BBrMV isolates to be the same virus and the "Ref" strain a different potyvirus from the other potyviruses analysed (Fig. 4). Three couples of primers defined from the "Ref" sequence were used to study the molecular variability of BBrMV in the field. The expected amplified fragments were obtained for 5 differents isolates. No significant variability was observed between the isolates and the "Ref" strain after sequencing. The "Ref" strain was thus representative of BBrMD in the field. The identification of specific primers for molecular detection is in progress. The presence of the potexvirus in BBrMD remains unexplained.
format book_section
topic_facet H20 - Maladies des plantes
Musa
virus des végétaux
Potyvirus
bractée
identification
immunologie
séquence nucléotidique
http://aims.fao.org/aos/agrovoc/c_4993
http://aims.fao.org/aos/agrovoc/c_5985
http://aims.fao.org/aos/agrovoc/c_32866
http://aims.fao.org/aos/agrovoc/c_1052
http://aims.fao.org/aos/agrovoc/c_3791
http://aims.fao.org/aos/agrovoc/c_3808
http://aims.fao.org/aos/agrovoc/c_27583
author Caruana, Marie-Line
Galzi, Serge
Laboureau, Nathalie
Bousalem, M.
Royer, Monique
Frutos, Roger
author_facet Caruana, Marie-Line
Galzi, Serge
Laboureau, Nathalie
Bousalem, M.
Royer, Monique
Frutos, Roger
author_sort Caruana, Marie-Line
title La maladie de la mosaïque des bractées des bananiers
title_short La maladie de la mosaïque des bractées des bananiers
title_full La maladie de la mosaïque des bractées des bananiers
title_fullStr La maladie de la mosaïque des bractées des bananiers
title_full_unstemmed La maladie de la mosaïque des bractées des bananiers
title_sort la maladie de la mosaïque des bractées des bananiers
publisher CIRAD
url http://agritrop.cirad.fr/392292/
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AT galziserge lamaladiedelamosaiquedesbracteesdesbananiers
AT laboureaunathalie lamaladiedelamosaiquedesbracteesdesbananiers
AT bousalemm lamaladiedelamosaiquedesbracteesdesbananiers
AT royermonique lamaladiedelamosaiquedesbracteesdesbananiers
AT frutosroger lamaladiedelamosaiquedesbracteesdesbananiers
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