Inter-Alu-like species-specific sequences in the Saccharum complex
#Alu# sequences constitute the most abundant family of short interspersed nuclear elements, SINEs, in the primate genome. The #Alu#-PCR method, which consists of amplification between "Alu# sequences, is usually applied in human genetics to provide polymorphic markers. Here we report the presence of #Alu#-like sequences in sugarcane and related species by applying the #Alu#-PCR-like method. Amplifications using a PCR primer defined in conserved regions of #Alu# human sequences lead to specific complex multiband profiles in all the #Saccharum# and related genera clones surveyed. The isolation and characterisation of the amplified genus-specific inter-#Alu#-like fragments allowed us to isolate repeated sequences that are specific for different genera of the #Saccharum# complex: MsCIR2 from #Miscanthus#, EaCIR6 and EaCIR7 from #Erianthus#, and SrCIR2 from #Saccharum#. Two PCR diagnostic tests were developed from the inter-#Alu#-like sequences MsCIR2 and EaCIR6, and proved efficient in identifying intergeneric hybrids #Saccharum# x #Miscanthus# or #Saccharum# x #Erianthus#, respectively. The present study illustrates how the #Alu#-PCR-like method could help investigating the origin of amphiploid species and monitoring introgression in plants. (Author's abstract)
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Subjects: | F30 - Génétique et amélioration des plantes, Saccharum, séquence nucléotidique, PCR, identification, empreinte ADN, http://aims.fao.org/aos/agrovoc/c_6725, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_34258, |
Online Access: | http://agritrop.cirad.fr/392201/ http://agritrop.cirad.fr/392201/1/392201.pdf |
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dig-cirad-fr-3922012024-01-28T00:00:27Z http://agritrop.cirad.fr/392201/ http://agritrop.cirad.fr/392201/ Inter-Alu-like species-specific sequences in the Saccharum complex. Alix Karine, Paulet Florence, Glaszmann Jean-Christophe, D'Hont Angélique. 1999. Theoretical and Applied Genetics, 99 : 962-968.https://doi.org/10.1007/s001220051403 <https://doi.org/10.1007/s001220051403> Inter-Alu-like species-specific sequences in the Saccharum complex Alix, Karine Paulet, Florence Glaszmann, Jean-Christophe D'Hont, Angélique eng 1999 Theoretical and Applied Genetics F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique PCR identification empreinte ADN http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 #Alu# sequences constitute the most abundant family of short interspersed nuclear elements, SINEs, in the primate genome. The #Alu#-PCR method, which consists of amplification between "Alu# sequences, is usually applied in human genetics to provide polymorphic markers. Here we report the presence of #Alu#-like sequences in sugarcane and related species by applying the #Alu#-PCR-like method. Amplifications using a PCR primer defined in conserved regions of #Alu# human sequences lead to specific complex multiband profiles in all the #Saccharum# and related genera clones surveyed. The isolation and characterisation of the amplified genus-specific inter-#Alu#-like fragments allowed us to isolate repeated sequences that are specific for different genera of the #Saccharum# complex: MsCIR2 from #Miscanthus#, EaCIR6 and EaCIR7 from #Erianthus#, and SrCIR2 from #Saccharum#. Two PCR diagnostic tests were developed from the inter-#Alu#-like sequences MsCIR2 and EaCIR6, and proved efficient in identifying intergeneric hybrids #Saccharum# x #Miscanthus# or #Saccharum# x #Erianthus#, respectively. The present study illustrates how the #Alu#-PCR-like method could help investigating the origin of amphiploid species and monitoring introgression in plants. (Author's abstract) article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/392201/1/392201.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s001220051403 10.1007/s001220051403 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=91217 info:eu-repo/semantics/altIdentifier/doi/10.1007/s001220051403 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s001220051403 |
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F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique PCR identification empreinte ADN http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique PCR identification empreinte ADN http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 |
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F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique PCR identification empreinte ADN http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique PCR identification empreinte ADN http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 Alix, Karine Paulet, Florence Glaszmann, Jean-Christophe D'Hont, Angélique Inter-Alu-like species-specific sequences in the Saccharum complex |
description |
#Alu# sequences constitute the most abundant family of short interspersed nuclear elements, SINEs, in the primate genome. The #Alu#-PCR method, which consists of amplification between "Alu# sequences, is usually applied in human genetics to provide polymorphic markers. Here we report the presence of #Alu#-like sequences in sugarcane and related species by applying the #Alu#-PCR-like method. Amplifications using a PCR primer defined in conserved regions of #Alu# human sequences lead to specific complex multiband profiles in all the #Saccharum# and related genera clones surveyed. The isolation and characterisation of the amplified genus-specific inter-#Alu#-like fragments allowed us to isolate repeated sequences that are specific for different genera of the #Saccharum# complex: MsCIR2 from #Miscanthus#, EaCIR6 and EaCIR7 from #Erianthus#, and SrCIR2 from #Saccharum#. Two PCR diagnostic tests were developed from the inter-#Alu#-like sequences MsCIR2 and EaCIR6, and proved efficient in identifying intergeneric hybrids #Saccharum# x #Miscanthus# or #Saccharum# x #Erianthus#, respectively. The present study illustrates how the #Alu#-PCR-like method could help investigating the origin of amphiploid species and monitoring introgression in plants. (Author's abstract) |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Saccharum séquence nucléotidique PCR identification empreinte ADN http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_34258 |
author |
Alix, Karine Paulet, Florence Glaszmann, Jean-Christophe D'Hont, Angélique |
author_facet |
Alix, Karine Paulet, Florence Glaszmann, Jean-Christophe D'Hont, Angélique |
author_sort |
Alix, Karine |
title |
Inter-Alu-like species-specific sequences in the Saccharum complex |
title_short |
Inter-Alu-like species-specific sequences in the Saccharum complex |
title_full |
Inter-Alu-like species-specific sequences in the Saccharum complex |
title_fullStr |
Inter-Alu-like species-specific sequences in the Saccharum complex |
title_full_unstemmed |
Inter-Alu-like species-specific sequences in the Saccharum complex |
title_sort |
inter-alu-like species-specific sequences in the saccharum complex |
url |
http://agritrop.cirad.fr/392201/ http://agritrop.cirad.fr/392201/1/392201.pdf |
work_keys_str_mv |
AT alixkarine interalulikespeciesspecificsequencesinthesaccharumcomplex AT pauletflorence interalulikespeciesspecificsequencesinthesaccharumcomplex AT glaszmannjeanchristophe interalulikespeciesspecificsequencesinthesaccharumcomplex AT dhontangelique interalulikespeciesspecificsequencesinthesaccharumcomplex |
_version_ |
1792491580287877120 |