Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting
Chromosomes counting and flow cytometry were used for assessing the ploidy level of various yam accessions of the INRA collection located in Guadeloupe. About 85 different clones were evaluated in the D. alata and D. cayenensis-rotundata and four other wild species related to D. cayenensis-rotundata. All the studied clones fitted in 4x, 6x and 8x ploidy levels. No diploid clone was found. Chromosomes counts and flow cytometry data led to the same results. The flow cytometry histograms for D. cayenensis-rotundata were not separated from those of its related wild species. Polyploidisation by fusion of 2n+n garnets was found to be unlikely for the two species D. cayenensis-rotundata and D. alata. Moreover, these results lead to the conclusion that the D. cayenensis-rotundata cultigens and the wild species analysed in this study may belong to the same gene pool.
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dig-cirad-fr-3916812023-07-05T13:08:30Z http://agritrop.cirad.fr/391681/ http://agritrop.cirad.fr/391681/ Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting. Gamiette Franciane, Bakry Frédéric, Ano Georges. 1999. Genetic Resources and Crop Evolution, 46 (1) : 19-27.https://doi.org/10.1023/A:1008649130567 <https://doi.org/10.1023/A:1008649130567> Researchers Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting Gamiette, Franciane Bakry, Frédéric Ano, Georges eng 1999 Genetic Resources and Crop Evolution F30 - Génétique et amélioration des plantes Chromosomes counting and flow cytometry were used for assessing the ploidy level of various yam accessions of the INRA collection located in Guadeloupe. About 85 different clones were evaluated in the D. alata and D. cayenensis-rotundata and four other wild species related to D. cayenensis-rotundata. All the studied clones fitted in 4x, 6x and 8x ploidy levels. No diploid clone was found. Chromosomes counts and flow cytometry data led to the same results. The flow cytometry histograms for D. cayenensis-rotundata were not separated from those of its related wild species. Polyploidisation by fusion of 2n+n garnets was found to be unlikely for the two species D. cayenensis-rotundata and D. alata. Moreover, these results lead to the conclusion that the D. cayenensis-rotundata cultigens and the wild species analysed in this study may belong to the same gene pool. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/391681/1/391681.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1023/A:1008649130567 10.1023/A:1008649130567 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=90857 info:eu-repo/semantics/altIdentifier/doi/10.1023/A:1008649130567 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1023/A:1008649130567 |
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F30 - Génétique et amélioration des plantes F30 - Génétique et amélioration des plantes Gamiette, Franciane Bakry, Frédéric Ano, Georges Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting |
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Chromosomes counting and flow cytometry were used for assessing the ploidy level of various yam accessions of the INRA collection located in Guadeloupe. About 85 different clones were evaluated in the D. alata and D. cayenensis-rotundata and four other wild species related to D. cayenensis-rotundata. All the studied clones fitted in 4x, 6x and 8x ploidy levels. No diploid clone was found. Chromosomes counts and flow cytometry data led to the same results. The flow cytometry histograms for D. cayenensis-rotundata were not separated from those of its related wild species. Polyploidisation by fusion of 2n+n garnets was found to be unlikely for the two species D. cayenensis-rotundata and D. alata. Moreover, these results lead to the conclusion that the D. cayenensis-rotundata cultigens and the wild species analysed in this study may belong to the same gene pool. |
| format |
article |
| topic_facet |
F30 - Génétique et amélioration des plantes |
| author |
Gamiette, Franciane Bakry, Frédéric Ano, Georges |
| author_facet |
Gamiette, Franciane Bakry, Frédéric Ano, Georges |
| author_sort |
Gamiette, Franciane |
| title |
Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting |
| title_short |
Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting |
| title_full |
Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting |
| title_fullStr |
Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting |
| title_full_unstemmed |
Ploidy determination of some yam species (Dioscorea spp.) by flow cytometry and conventional chromosomes counting |
| title_sort |
ploidy determination of some yam species (dioscorea spp.) by flow cytometry and conventional chromosomes counting |
| url |
http://agritrop.cirad.fr/391681/ http://agritrop.cirad.fr/391681/1/391681.pdf |
| work_keys_str_mv |
AT gamiettefranciane ploidydeterminationofsomeyamspeciesdioscoreasppbyflowcytometryandconventionalchromosomescounting AT bakryfrederic ploidydeterminationofsomeyamspeciesdioscoreasppbyflowcytometryandconventionalchromosomescounting AT anogeorges ploidydeterminationofsomeyamspeciesdioscoreasppbyflowcytometryandconventionalchromosomescounting |
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