Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system
An assay system was developed for the adult silverleaf whitefly, #Bemisia argentifolii# Bellows & Perring (Homoptera : Aleyrodidae). This practical device was constructed from standard disposable laboratory materials. Whiteflies were harvested directly from the leaf and into a collection vial by vacuum aspiration, minimizing physical damage to the insect. Insects were fed throueh a cellulose mixed-ester membrane on a diet of 20-27% sucrose alone or sucrose in an extract of zucchini (#Curcurbita moschata# Duchense). Mortality and honeydew production were scored. At 22-25 °C and 50-55%, relative humidity, control mortality generally remained at or below 15% during a 48 h assay period. The bioassay system was first tested using the insecticide, Imidacloprid, then used to screen a number of natural products with potential insecticidal activity against the whitefly. Destruxins extracted from the entornopathogenic fungus, #Metarhizium anisopliae#, and the natural insccticide/nematicide, Ivermectin, as well as bee venom and two of its components, melittin and phospholipase A,, were found to be toxic to #B. argentifolii#. Five lectins, #Bacillus thuringiensis# toxins, gossypol, an extract of #Paeciliomyces fumosoroseus#, wasp and scorpion venom, and a trypsin inhibitor were not found to be insecticidal to adult #B. argentifolii#.
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Language: | eng |
Subjects: | H10 - Ravageurs des plantes, Bemisia argentifolii, toxine bactérienne, lutte anti-insecte, biopesticide, http://aims.fao.org/aos/agrovoc/c_36596, http://aims.fao.org/aos/agrovoc/c_9047, http://aims.fao.org/aos/agrovoc/c_3885, http://aims.fao.org/aos/agrovoc/c_27467, |
Online Access: | http://agritrop.cirad.fr/391532/ http://agritrop.cirad.fr/391532/1/ID391532.pdf |
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dig-cirad-fr-3915322024-01-27T23:43:11Z http://agritrop.cirad.fr/391532/ http://agritrop.cirad.fr/391532/ Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system. Davidson E.W., Patron R.B.R., Lacey Lawrence A., Frutos Roger, Vey A., Hendrix D.L.. 1996. Entomologia Experimentalis et Applicata, 79 : 25-32.https://doi.org/10.1111/j.1570-7458.1996.tb00805.x <https://doi.org/10.1111/j.1570-7458.1996.tb00805.x> Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system Davidson, E.W. Patron, R.B.R. Lacey, Lawrence A. Frutos, Roger Vey, A. Hendrix, D.L. eng 1996 Entomologia Experimentalis et Applicata H10 - Ravageurs des plantes Bemisia argentifolii toxine bactérienne lutte anti-insecte biopesticide http://aims.fao.org/aos/agrovoc/c_36596 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_27467 An assay system was developed for the adult silverleaf whitefly, #Bemisia argentifolii# Bellows & Perring (Homoptera : Aleyrodidae). This practical device was constructed from standard disposable laboratory materials. Whiteflies were harvested directly from the leaf and into a collection vial by vacuum aspiration, minimizing physical damage to the insect. Insects were fed throueh a cellulose mixed-ester membrane on a diet of 20-27% sucrose alone or sucrose in an extract of zucchini (#Curcurbita moschata# Duchense). Mortality and honeydew production were scored. At 22-25 °C and 50-55%, relative humidity, control mortality generally remained at or below 15% during a 48 h assay period. The bioassay system was first tested using the insecticide, Imidacloprid, then used to screen a number of natural products with potential insecticidal activity against the whitefly. Destruxins extracted from the entornopathogenic fungus, #Metarhizium anisopliae#, and the natural insccticide/nematicide, Ivermectin, as well as bee venom and two of its components, melittin and phospholipase A,, were found to be toxic to #B. argentifolii#. Five lectins, #Bacillus thuringiensis# toxins, gossypol, an extract of #Paeciliomyces fumosoroseus#, wasp and scorpion venom, and a trypsin inhibitor were not found to be insecticidal to adult #B. argentifolii#. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/391532/1/ID391532.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/j.1570-7458.1996.tb00805.x 10.1111/j.1570-7458.1996.tb00805.x http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=90718 info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1570-7458.1996.tb00805.x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/j.1570-7458.1996.tb00805.x |
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H10 - Ravageurs des plantes Bemisia argentifolii toxine bactérienne lutte anti-insecte biopesticide http://aims.fao.org/aos/agrovoc/c_36596 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_27467 H10 - Ravageurs des plantes Bemisia argentifolii toxine bactérienne lutte anti-insecte biopesticide http://aims.fao.org/aos/agrovoc/c_36596 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_27467 |
spellingShingle |
H10 - Ravageurs des plantes Bemisia argentifolii toxine bactérienne lutte anti-insecte biopesticide http://aims.fao.org/aos/agrovoc/c_36596 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_27467 H10 - Ravageurs des plantes Bemisia argentifolii toxine bactérienne lutte anti-insecte biopesticide http://aims.fao.org/aos/agrovoc/c_36596 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_27467 Davidson, E.W. Patron, R.B.R. Lacey, Lawrence A. Frutos, Roger Vey, A. Hendrix, D.L. Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system |
description |
An assay system was developed for the adult silverleaf whitefly, #Bemisia argentifolii# Bellows & Perring (Homoptera : Aleyrodidae). This practical device was constructed from standard disposable laboratory materials. Whiteflies were harvested directly from the leaf and into a collection vial by vacuum aspiration, minimizing physical damage to the insect. Insects were fed throueh a cellulose mixed-ester membrane on a diet of 20-27% sucrose alone or sucrose in an extract of zucchini (#Curcurbita moschata# Duchense). Mortality and honeydew production were scored. At 22-25 °C and 50-55%, relative humidity, control mortality generally remained at or below 15% during a 48 h assay period. The bioassay system was first tested using the insecticide, Imidacloprid, then used to screen a number of natural products with potential insecticidal activity against the whitefly. Destruxins extracted from the entornopathogenic fungus, #Metarhizium anisopliae#, and the natural insccticide/nematicide, Ivermectin, as well as bee venom and two of its components, melittin and phospholipase A,, were found to be toxic to #B. argentifolii#. Five lectins, #Bacillus thuringiensis# toxins, gossypol, an extract of #Paeciliomyces fumosoroseus#, wasp and scorpion venom, and a trypsin inhibitor were not found to be insecticidal to adult #B. argentifolii#. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes Bemisia argentifolii toxine bactérienne lutte anti-insecte biopesticide http://aims.fao.org/aos/agrovoc/c_36596 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_27467 |
author |
Davidson, E.W. Patron, R.B.R. Lacey, Lawrence A. Frutos, Roger Vey, A. Hendrix, D.L. |
author_facet |
Davidson, E.W. Patron, R.B.R. Lacey, Lawrence A. Frutos, Roger Vey, A. Hendrix, D.L. |
author_sort |
Davidson, E.W. |
title |
Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system |
title_short |
Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system |
title_full |
Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system |
title_fullStr |
Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system |
title_full_unstemmed |
Activity of natural toxins against the silverleaf whitefly, Bemisia argentifolii, using a novel feeding bioassay system |
title_sort |
activity of natural toxins against the silverleaf whitefly, bemisia argentifolii, using a novel feeding bioassay system |
url |
http://agritrop.cirad.fr/391532/ http://agritrop.cirad.fr/391532/1/ID391532.pdf |
work_keys_str_mv |
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