Plant regeneration from long-term callus culture of 'AAA'-Group dessert banana
The banana plant is one of the most widely cultivated crops in the world. However, banana breeding has been a slow process, due to the low seed set and low germination rates. Selection of useful somaclonal variations and genetic transformation in cells or calluses are promising techniques to accelerate the breeding process. Therefore, callus culture was carried out, aiming the establishment of one protocol for plant regeneration, to be used in banana breeding program. Leaf sheath disks of 'Nanicao' banana (Musa sp., AAA group, Cavendish subgroup) were cultured on a Murashige and Skoog (MS) basal medium supplemented with activated charcoal (0.2%), MES (2 [N-morpholino] ethanesulfonic acid) (15.3 mM), arginine (300 mM), Picloram (414 micro M) and 2iP (2-isopentenyl adenine) (492 micronM). Globular calluses developed on the leaf tissue were subcultured in the same medium, acquiring a friable and translucid appearance after one and a half year of culture. The friable calluses were transferred to the medium without growth regulators and arginine, and supplemented with casein hydrolysate (0.05%), where they formed embryo-like structures after transference to light. From these structures, shoots with roots were obtained and plantlets developed. The plant regeneration protocol shown here may be useful to banana breeding via somaclonal variation.
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Subjects: | F30 - Génétique et amélioration des plantes, Musa, amélioration des plantes, culture de tissu, cal, variation somaclonale, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_5956, http://aims.fao.org/aos/agrovoc/c_7789, http://aims.fao.org/aos/agrovoc/c_24576, http://aims.fao.org/aos/agrovoc/c_34127, |
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dig-cirad-fr-3908042024-01-27T23:35:38Z http://agritrop.cirad.fr/390804/ http://agritrop.cirad.fr/390804/ Plant regeneration from long-term callus culture of 'AAA'-Group dessert banana. Da Silva Conceicao A., Matsumoto Kazumitsu, Bakry Frédéric, Berno Souza R.B.. 1998. Pesquisa Agropecuária Brasileira, 33 (8) : 1291-1296. Plant regeneration from long-term callus culture of 'AAA'-Group dessert banana Da Silva Conceicao, A. Matsumoto, Kazumitsu Bakry, Frédéric Berno Souza, R.B. eng 1998 Pesquisa Agropecuária Brasileira F30 - Génétique et amélioration des plantes Musa amélioration des plantes culture de tissu cal variation somaclonale http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5956 http://aims.fao.org/aos/agrovoc/c_7789 http://aims.fao.org/aos/agrovoc/c_24576 http://aims.fao.org/aos/agrovoc/c_34127 The banana plant is one of the most widely cultivated crops in the world. However, banana breeding has been a slow process, due to the low seed set and low germination rates. Selection of useful somaclonal variations and genetic transformation in cells or calluses are promising techniques to accelerate the breeding process. Therefore, callus culture was carried out, aiming the establishment of one protocol for plant regeneration, to be used in banana breeding program. Leaf sheath disks of 'Nanicao' banana (Musa sp., AAA group, Cavendish subgroup) were cultured on a Murashige and Skoog (MS) basal medium supplemented with activated charcoal (0.2%), MES (2 [N-morpholino] ethanesulfonic acid) (15.3 mM), arginine (300 mM), Picloram (414 micro M) and 2iP (2-isopentenyl adenine) (492 micronM). Globular calluses developed on the leaf tissue were subcultured in the same medium, acquiring a friable and translucid appearance after one and a half year of culture. The friable calluses were transferred to the medium without growth regulators and arginine, and supplemented with casein hydrolysate (0.05%), where they formed embryo-like structures after transference to light. From these structures, shoots with roots were obtained and plantlets developed. The plant regeneration protocol shown here may be useful to banana breeding via somaclonal variation. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=90145 |
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F30 - Génétique et amélioration des plantes Musa amélioration des plantes culture de tissu cal variation somaclonale http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5956 http://aims.fao.org/aos/agrovoc/c_7789 http://aims.fao.org/aos/agrovoc/c_24576 http://aims.fao.org/aos/agrovoc/c_34127 F30 - Génétique et amélioration des plantes Musa amélioration des plantes culture de tissu cal variation somaclonale http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5956 http://aims.fao.org/aos/agrovoc/c_7789 http://aims.fao.org/aos/agrovoc/c_24576 http://aims.fao.org/aos/agrovoc/c_34127 |
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F30 - Génétique et amélioration des plantes Musa amélioration des plantes culture de tissu cal variation somaclonale http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5956 http://aims.fao.org/aos/agrovoc/c_7789 http://aims.fao.org/aos/agrovoc/c_24576 http://aims.fao.org/aos/agrovoc/c_34127 F30 - Génétique et amélioration des plantes Musa amélioration des plantes culture de tissu cal variation somaclonale http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5956 http://aims.fao.org/aos/agrovoc/c_7789 http://aims.fao.org/aos/agrovoc/c_24576 http://aims.fao.org/aos/agrovoc/c_34127 Da Silva Conceicao, A. Matsumoto, Kazumitsu Bakry, Frédéric Berno Souza, R.B. Plant regeneration from long-term callus culture of 'AAA'-Group dessert banana |
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The banana plant is one of the most widely cultivated crops in the world. However, banana breeding has been a slow process, due to the low seed set and low germination rates. Selection of useful somaclonal variations and genetic transformation in cells or calluses are promising techniques to accelerate the breeding process. Therefore, callus culture was carried out, aiming the establishment of one protocol for plant regeneration, to be used in banana breeding program. Leaf sheath disks of 'Nanicao' banana (Musa sp., AAA group, Cavendish subgroup) were cultured on a Murashige and Skoog (MS) basal medium supplemented with activated charcoal (0.2%), MES (2 [N-morpholino] ethanesulfonic acid) (15.3 mM), arginine (300 mM), Picloram (414 micro M) and 2iP (2-isopentenyl adenine) (492 micronM). Globular calluses developed on the leaf tissue were subcultured in the same medium, acquiring a friable and translucid appearance after one and a half year of culture. The friable calluses were transferred to the medium without growth regulators and arginine, and supplemented with casein hydrolysate (0.05%), where they formed embryo-like structures after transference to light. From these structures, shoots with roots were obtained and plantlets developed. The plant regeneration protocol shown here may be useful to banana breeding via somaclonal variation. |
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article |
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F30 - Génétique et amélioration des plantes Musa amélioration des plantes culture de tissu cal variation somaclonale http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_5956 http://aims.fao.org/aos/agrovoc/c_7789 http://aims.fao.org/aos/agrovoc/c_24576 http://aims.fao.org/aos/agrovoc/c_34127 |
author |
Da Silva Conceicao, A. Matsumoto, Kazumitsu Bakry, Frédéric Berno Souza, R.B. |
author_facet |
Da Silva Conceicao, A. Matsumoto, Kazumitsu Bakry, Frédéric Berno Souza, R.B. |
author_sort |
Da Silva Conceicao, A. |
title |
Plant regeneration from long-term callus culture of 'AAA'-Group dessert banana |
title_short |
Plant regeneration from long-term callus culture of 'AAA'-Group dessert banana |
title_full |
Plant regeneration from long-term callus culture of 'AAA'-Group dessert banana |
title_fullStr |
Plant regeneration from long-term callus culture of 'AAA'-Group dessert banana |
title_full_unstemmed |
Plant regeneration from long-term callus culture of 'AAA'-Group dessert banana |
title_sort |
plant regeneration from long-term callus culture of 'aaa'-group dessert banana |
url |
http://agritrop.cirad.fr/390804/ |
work_keys_str_mv |
AT dasilvaconceicaoa plantregenerationfromlongtermcalluscultureofaaagroupdessertbanana AT matsumotokazumitsu plantregenerationfromlongtermcalluscultureofaaagroupdessertbanana AT bakryfrederic plantregenerationfromlongtermcalluscultureofaaagroupdessertbanana AT bernosouzarb plantregenerationfromlongtermcalluscultureofaaagroupdessertbanana |
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1792491434196074496 |