Genetic transformation of a Thai cotton variety (Sri Somorong 60) for insect resistance
Cotton production in Thailand has been decreased by nearly 50% over the last fifteen years. The cotton bollworm, #Helicoverpa armigera# Hubner is the key pest in Thai cotton cropping system. One strategy to circumvent this problem is to transfer to cotton plants genes encoding for #Bacillus thuringiensis# endotoxins which have been identified as very toxic towards this insect. Techniques were developed for embryogeni callus induction, transformation and regeneration of Sri Somrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyl explants on to zeatin and NAA containing MS medium and subculturing on to hormone free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Somrong 60 with synthetic crylA(b) gene was accomplished by using #Agrobacterium tumefaciens# strain C 58: pG2260. The selection of resistant calli which were obtained at frequencies varying from 3 to 50%, was accomplished on kanamycin containing medium. The molecular analysis of recovered plants showed the integration of the transferred gene. Bioassays conducted with leaves of different transgenic plant lines showed different level of resistance towards #Helicoverpa armigera# first instar larvae. The association of B.t genes with other tolerance characters existing in the cotton germplasm is in progress using conventional breeding techniques.
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Subjects: | F30 - Génétique et amélioration des plantes, Gossypium, variété, transformation génétique, résistance aux organismes nuisibles, Embryogénèse somatique, Bacillus thuringiensis, transfert de gène, Agrobacterium tumefaciens, régénération in vitro, http://aims.fao.org/aos/agrovoc/c_3335, http://aims.fao.org/aos/agrovoc/c_8157, http://aims.fao.org/aos/agrovoc/c_3220, http://aims.fao.org/aos/agrovoc/c_5731, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_24846, http://aims.fao.org/aos/agrovoc/c_27444, http://aims.fao.org/aos/agrovoc/c_34114, http://aims.fao.org/aos/agrovoc/c_7701, |
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dig-cirad-fr-3903992024-01-27T23:23:04Z http://agritrop.cirad.fr/390399/ http://agritrop.cirad.fr/390399/ Genetic transformation of a Thai cotton variety (Sri Somorong 60) for insect resistance. Chaïr Hâna, Kuhapituktum R., Attathom T., Giband Marc, Pannetier Catherine, Attathom S.. 1998. In : Asia-Pacific Conference on Agricultural Biotechnology. To Field and Market. 4. s.l. : s.n., 5 p. Asia-Pacific Conference on Agricultural Biotechnology. To Field and Market. 4, Darwin, Australie, 13 Juillet 1998/16 Juillet 1998. Genetic transformation of a Thai cotton variety (Sri Somorong 60) for insect resistance Chaïr, Hâna Kuhapituktum, R. Attathom, T. Giband, Marc Pannetier, Catherine Attathom, S. eng 1998 s.n. Asia-Pacific Conference on Agricultural Biotechnology. To Field and Market. 4 F30 - Génétique et amélioration des plantes Gossypium variété transformation génétique résistance aux organismes nuisibles Embryogénèse somatique Bacillus thuringiensis transfert de gène Agrobacterium tumefaciens régénération in vitro http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_34114 Thaïlande http://aims.fao.org/aos/agrovoc/c_7701 Cotton production in Thailand has been decreased by nearly 50% over the last fifteen years. The cotton bollworm, #Helicoverpa armigera# Hubner is the key pest in Thai cotton cropping system. One strategy to circumvent this problem is to transfer to cotton plants genes encoding for #Bacillus thuringiensis# endotoxins which have been identified as very toxic towards this insect. Techniques were developed for embryogeni callus induction, transformation and regeneration of Sri Somrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyl explants on to zeatin and NAA containing MS medium and subculturing on to hormone free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Somrong 60 with synthetic crylA(b) gene was accomplished by using #Agrobacterium tumefaciens# strain C 58: pG2260. The selection of resistant calli which were obtained at frequencies varying from 3 to 50%, was accomplished on kanamycin containing medium. The molecular analysis of recovered plants showed the integration of the transferred gene. Bioassays conducted with leaves of different transgenic plant lines showed different level of resistance towards #Helicoverpa armigera# first instar larvae. The association of B.t genes with other tolerance characters existing in the cotton germplasm is in progress using conventional breeding techniques. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=90073 |
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F30 - Génétique et amélioration des plantes Gossypium variété transformation génétique résistance aux organismes nuisibles Embryogénèse somatique Bacillus thuringiensis transfert de gène Agrobacterium tumefaciens régénération in vitro http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_7701 F30 - Génétique et amélioration des plantes Gossypium variété transformation génétique résistance aux organismes nuisibles Embryogénèse somatique Bacillus thuringiensis transfert de gène Agrobacterium tumefaciens régénération in vitro http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_7701 |
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F30 - Génétique et amélioration des plantes Gossypium variété transformation génétique résistance aux organismes nuisibles Embryogénèse somatique Bacillus thuringiensis transfert de gène Agrobacterium tumefaciens régénération in vitro http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_7701 F30 - Génétique et amélioration des plantes Gossypium variété transformation génétique résistance aux organismes nuisibles Embryogénèse somatique Bacillus thuringiensis transfert de gène Agrobacterium tumefaciens régénération in vitro http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_7701 Chaïr, Hâna Kuhapituktum, R. Attathom, T. Giband, Marc Pannetier, Catherine Attathom, S. Genetic transformation of a Thai cotton variety (Sri Somorong 60) for insect resistance |
description |
Cotton production in Thailand has been decreased by nearly 50% over the last fifteen years. The cotton bollworm, #Helicoverpa armigera# Hubner is the key pest in Thai cotton cropping system. One strategy to circumvent this problem is to transfer to cotton plants genes encoding for #Bacillus thuringiensis# endotoxins which have been identified as very toxic towards this insect. Techniques were developed for embryogeni callus induction, transformation and regeneration of Sri Somrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyl explants on to zeatin and NAA containing MS medium and subculturing on to hormone free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Somrong 60 with synthetic crylA(b) gene was accomplished by using #Agrobacterium tumefaciens# strain C 58: pG2260. The selection of resistant calli which were obtained at frequencies varying from 3 to 50%, was accomplished on kanamycin containing medium. The molecular analysis of recovered plants showed the integration of the transferred gene. Bioassays conducted with leaves of different transgenic plant lines showed different level of resistance towards #Helicoverpa armigera# first instar larvae. The association of B.t genes with other tolerance characters existing in the cotton germplasm is in progress using conventional breeding techniques. |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes Gossypium variété transformation génétique résistance aux organismes nuisibles Embryogénèse somatique Bacillus thuringiensis transfert de gène Agrobacterium tumefaciens régénération in vitro http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_3220 http://aims.fao.org/aos/agrovoc/c_5731 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_27444 http://aims.fao.org/aos/agrovoc/c_34114 http://aims.fao.org/aos/agrovoc/c_7701 |
author |
Chaïr, Hâna Kuhapituktum, R. Attathom, T. Giband, Marc Pannetier, Catherine Attathom, S. |
author_facet |
Chaïr, Hâna Kuhapituktum, R. Attathom, T. Giband, Marc Pannetier, Catherine Attathom, S. |
author_sort |
Chaïr, Hâna |
title |
Genetic transformation of a Thai cotton variety (Sri Somorong 60) for insect resistance |
title_short |
Genetic transformation of a Thai cotton variety (Sri Somorong 60) for insect resistance |
title_full |
Genetic transformation of a Thai cotton variety (Sri Somorong 60) for insect resistance |
title_fullStr |
Genetic transformation of a Thai cotton variety (Sri Somorong 60) for insect resistance |
title_full_unstemmed |
Genetic transformation of a Thai cotton variety (Sri Somorong 60) for insect resistance |
title_sort |
genetic transformation of a thai cotton variety (sri somorong 60) for insect resistance |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/390399/ |
work_keys_str_mv |
AT chairhana genetictransformationofathaicottonvarietysrisomorong60forinsectresistance AT kuhapituktumr genetictransformationofathaicottonvarietysrisomorong60forinsectresistance AT attathomt genetictransformationofathaicottonvarietysrisomorong60forinsectresistance AT gibandmarc genetictransformationofathaicottonvarietysrisomorong60forinsectresistance AT pannetiercatherine genetictransformationofathaicottonvarietysrisomorong60forinsectresistance AT attathoms genetictransformationofathaicottonvarietysrisomorong60forinsectresistance |
_version_ |
1792491353211404288 |