Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross
We have started genetic mapping of the citrus genome by segregation analysis of different molecular markers (RAPDS, RFLP and isozymes) in the progeny of FAO 30573 hybrid (#Citrus reshni# x #Pancirus trifoliata#) and pummelo (#C. grandi#s) used respectively as paternal and maternal parents. A total DNA library and a cDNA library were constructed from sweet orange (#C. sinensis#) leaves of the Valencia Late clone. The screening of probes w.as made on the parent genotypes using 4 restriction enzymes. Among 85 tested probes, 85 % of cDNA probes and 10 % of genomic probes revealed polymorphic patterns. Segregation analysis w.as performed w.ith the MapMaker program. The map of the intergeneric hybrid comprised 95 foci linked in 12 groups : 7 isozymes, 45 cDNA/RFLPs, 1 rDNA/RFLPs, 40 RAPDs and 2 inter-microsatellite sequences. The map of pummelo comprised 34 markers making 7 linkage groups : 3 isozymes, 13 cDNA/RFLP, 15 RAPDs and 3 inter-microsatellite sequences. Only the first map shows evidence of skewed segregation within some linkage groups (37.5% of total markers). Some applications of citrus genetic maps are given.
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Subjects: | F30 - Génétique et amélioration des plantes, Citrus, carte génétique, marqueur génétique, RAPD, RFLP, isoenzyme, ségrégation, http://aims.fao.org/aos/agrovoc/c_1637, http://aims.fao.org/aos/agrovoc/c_24002, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_36341, http://aims.fao.org/aos/agrovoc/c_34255, http://aims.fao.org/aos/agrovoc/c_15987, http://aims.fao.org/aos/agrovoc/c_6947, |
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dig-cirad-fr-3902262024-01-27T23:17:40Z http://agritrop.cirad.fr/390226/ http://agritrop.cirad.fr/390226/ Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross. Luro François, Laigret Frédéric, Lorieux Mathias, Ollitrault Patrick. 1996. In : Proceedings International Society Citriculture. Volume 2. s.l. : s.n., 862-866. International Citrus Congress. 8, Sun City, Afrique du Sud, 12 Mai 1996/17 Mai 1996. Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross Luro, François Laigret, Frédéric Lorieux, Mathias Ollitrault, Patrick eng 1996 s.n. Proceedings International Society Citriculture. Volume 2 F30 - Génétique et amélioration des plantes Citrus carte génétique marqueur génétique RAPD RFLP isoenzyme ségrégation http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_6947 We have started genetic mapping of the citrus genome by segregation analysis of different molecular markers (RAPDS, RFLP and isozymes) in the progeny of FAO 30573 hybrid (#Citrus reshni# x #Pancirus trifoliata#) and pummelo (#C. grandi#s) used respectively as paternal and maternal parents. A total DNA library and a cDNA library were constructed from sweet orange (#C. sinensis#) leaves of the Valencia Late clone. The screening of probes w.as made on the parent genotypes using 4 restriction enzymes. Among 85 tested probes, 85 % of cDNA probes and 10 % of genomic probes revealed polymorphic patterns. Segregation analysis w.as performed w.ith the MapMaker program. The map of the intergeneric hybrid comprised 95 foci linked in 12 groups : 7 isozymes, 45 cDNA/RFLPs, 1 rDNA/RFLPs, 40 RAPDs and 2 inter-microsatellite sequences. The map of pummelo comprised 34 markers making 7 linkage groups : 3 isozymes, 13 cDNA/RFLP, 15 RAPDs and 3 inter-microsatellite sequences. Only the first map shows evidence of skewed segregation within some linkage groups (37.5% of total markers). Some applications of citrus genetic maps are given. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=89865 |
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F30 - Génétique et amélioration des plantes Citrus carte génétique marqueur génétique RAPD RFLP isoenzyme ségrégation http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_6947 F30 - Génétique et amélioration des plantes Citrus carte génétique marqueur génétique RAPD RFLP isoenzyme ségrégation http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_6947 |
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F30 - Génétique et amélioration des plantes Citrus carte génétique marqueur génétique RAPD RFLP isoenzyme ségrégation http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_6947 F30 - Génétique et amélioration des plantes Citrus carte génétique marqueur génétique RAPD RFLP isoenzyme ségrégation http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_6947 Luro, François Laigret, Frédéric Lorieux, Mathias Ollitrault, Patrick Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross |
description |
We have started genetic mapping of the citrus genome by segregation analysis of different molecular markers (RAPDS, RFLP and isozymes) in the progeny of FAO 30573 hybrid (#Citrus reshni# x #Pancirus trifoliata#) and pummelo (#C. grandi#s) used respectively as paternal and maternal parents. A total DNA library and a cDNA library were constructed from sweet orange (#C. sinensis#) leaves of the Valencia Late clone. The screening of probes w.as made on the parent genotypes using 4 restriction enzymes. Among 85 tested probes, 85 % of cDNA probes and 10 % of genomic probes revealed polymorphic patterns. Segregation analysis w.as performed w.ith the MapMaker program. The map of the intergeneric hybrid comprised 95 foci linked in 12 groups : 7 isozymes, 45 cDNA/RFLPs, 1 rDNA/RFLPs, 40 RAPDs and 2 inter-microsatellite sequences. The map of pummelo comprised 34 markers making 7 linkage groups : 3 isozymes, 13 cDNA/RFLP, 15 RAPDs and 3 inter-microsatellite sequences. Only the first map shows evidence of skewed segregation within some linkage groups (37.5% of total markers). Some applications of citrus genetic maps are given. |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes Citrus carte génétique marqueur génétique RAPD RFLP isoenzyme ségrégation http://aims.fao.org/aos/agrovoc/c_1637 http://aims.fao.org/aos/agrovoc/c_24002 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_36341 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_15987 http://aims.fao.org/aos/agrovoc/c_6947 |
author |
Luro, François Laigret, Frédéric Lorieux, Mathias Ollitrault, Patrick |
author_facet |
Luro, François Laigret, Frédéric Lorieux, Mathias Ollitrault, Patrick |
author_sort |
Luro, François |
title |
Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross |
title_short |
Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross |
title_full |
Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross |
title_fullStr |
Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross |
title_full_unstemmed |
Citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross |
title_sort |
citrus genome mapping with molecular markers : two maps obtained by segregation analysis of progeny of one intergeneric cross |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/390226/ |
work_keys_str_mv |
AT lurofrancois citrusgenomemappingwithmolecularmarkerstwomapsobtainedbysegregationanalysisofprogenyofoneintergenericcross AT laigretfrederic citrusgenomemappingwithmolecularmarkerstwomapsobtainedbysegregationanalysisofprogenyofoneintergenericcross AT lorieuxmathias citrusgenomemappingwithmolecularmarkerstwomapsobtainedbysegregationanalysisofprogenyofoneintergenericcross AT ollitraultpatrick citrusgenomemappingwithmolecularmarkerstwomapsobtainedbysegregationanalysisofprogenyofoneintergenericcross |
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1792491320984469504 |