Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes
Mycoplasma capricolum subsp. capripneumoniae belongs to the so-called Mycoplasma mycoides cluster and is the causal agent of contagious caprine pleuropneumonia (CCPP). All members of the M. mycoides cluster have two rRNA operons. The sequences of the 16S rRNA genes of both rRNA operons from 20 strains of M. capricolum subsp. capripneumoniae of different geographical origins in Africa and Asia were determined. Nucleotide differences which were present in only one of the two operons (polymorphisms) were detected in 24 positions. The polymorphisms were not randomly distributed in the 16S rRNA genes, and some of them were found in regions of low evolutionary variability. Interestingly, 11 polymorphisms were found in all the M. capricolum subsp. capripneumoniae strains, thus defining a putative ancestor. A sequence length difference between the 16S rRNA genes in a poly(A) region and 12 additional polymorphisms were found in only one or some of the strains. A phylogenetic tree was constructed by comparative analysis of the polymorphisme, and this tree revealed two distinct fines of descent. The nucleotide substitution rate of strains within line II was up to 50% higher than within line I. A tree was also constructed from individual operonal 16S rRNA sequences, and the sequences of the two operons were found to form two distinct clades. The topologies of both clades were strikingly similar, which supports the use of 16S rRNA sequence data from homologous operons for phylogenetic studies. The strain-specific polymorphism patterns of the 16S rRNA genes of M. capricolum subsp. capripneumoniae may be used as epidemiological markers for CCPP.
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Subjects: | L73 - Maladies des animaux, Mycoplasma capricolum, épidémiologie, Mycoplasma mycoides, mycoplasmose, arn, séquence nucléotidique, marqueur génétique, http://aims.fao.org/aos/agrovoc/c_27242, http://aims.fao.org/aos/agrovoc/c_2615, http://aims.fao.org/aos/agrovoc/c_23951, http://aims.fao.org/aos/agrovoc/c_5022, http://aims.fao.org/aos/agrovoc/c_6618, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_24030, |
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dig-cirad-fr-3901252024-01-27T23:14:41Z http://agritrop.cirad.fr/390125/ http://agritrop.cirad.fr/390125/ Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes. Pettersson B., Bölske Göran, Thiaucourt François, Uhlén M., Johansson Karl-Erik. 1998. Journal of Bacteriology, 180 (9) : 2350-2358.https://doi.org/10.1128/JB.180.9.2350-2358.1998 <https://doi.org/10.1128/JB.180.9.2350-2358.1998> Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes Pettersson, B. Bölske, Göran Thiaucourt, François Uhlén, M. Johansson, Karl-Erik eng 1998 Journal of Bacteriology L73 - Maladies des animaux Mycoplasma capricolum épidémiologie Mycoplasma mycoides mycoplasmose arn séquence nucléotidique marqueur génétique http://aims.fao.org/aos/agrovoc/c_27242 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_24030 Mycoplasma capricolum subsp. capripneumoniae belongs to the so-called Mycoplasma mycoides cluster and is the causal agent of contagious caprine pleuropneumonia (CCPP). All members of the M. mycoides cluster have two rRNA operons. The sequences of the 16S rRNA genes of both rRNA operons from 20 strains of M. capricolum subsp. capripneumoniae of different geographical origins in Africa and Asia were determined. Nucleotide differences which were present in only one of the two operons (polymorphisms) were detected in 24 positions. The polymorphisms were not randomly distributed in the 16S rRNA genes, and some of them were found in regions of low evolutionary variability. Interestingly, 11 polymorphisms were found in all the M. capricolum subsp. capripneumoniae strains, thus defining a putative ancestor. A sequence length difference between the 16S rRNA genes in a poly(A) region and 12 additional polymorphisms were found in only one or some of the strains. A phylogenetic tree was constructed by comparative analysis of the polymorphisme, and this tree revealed two distinct fines of descent. The nucleotide substitution rate of strains within line II was up to 50% higher than within line I. A tree was also constructed from individual operonal 16S rRNA sequences, and the sequences of the two operons were found to form two distinct clades. The topologies of both clades were strikingly similar, which supports the use of 16S rRNA sequence data from homologous operons for phylogenetic studies. The strain-specific polymorphism patterns of the 16S rRNA genes of M. capricolum subsp. capripneumoniae may be used as epidemiological markers for CCPP. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/390125/1/390125.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1128/JB.180.9.2350-2358.1998 10.1128/JB.180.9.2350-2358.1998 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=90102 info:eu-repo/semantics/altIdentifier/doi/10.1128/JB.180.9.2350-2358.1998 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1128/JB.180.9.2350-2358.1998 |
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L73 - Maladies des animaux Mycoplasma capricolum épidémiologie Mycoplasma mycoides mycoplasmose arn séquence nucléotidique marqueur génétique http://aims.fao.org/aos/agrovoc/c_27242 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_24030 L73 - Maladies des animaux Mycoplasma capricolum épidémiologie Mycoplasma mycoides mycoplasmose arn séquence nucléotidique marqueur génétique http://aims.fao.org/aos/agrovoc/c_27242 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_24030 |
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L73 - Maladies des animaux Mycoplasma capricolum épidémiologie Mycoplasma mycoides mycoplasmose arn séquence nucléotidique marqueur génétique http://aims.fao.org/aos/agrovoc/c_27242 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_24030 L73 - Maladies des animaux Mycoplasma capricolum épidémiologie Mycoplasma mycoides mycoplasmose arn séquence nucléotidique marqueur génétique http://aims.fao.org/aos/agrovoc/c_27242 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_24030 Pettersson, B. Bölske, Göran Thiaucourt, François Uhlén, M. Johansson, Karl-Erik Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes |
description |
Mycoplasma capricolum subsp. capripneumoniae belongs to the so-called Mycoplasma mycoides cluster and is the causal agent of contagious caprine pleuropneumonia (CCPP). All members of the M. mycoides cluster have two rRNA operons. The sequences of the 16S rRNA genes of both rRNA operons from 20 strains of M. capricolum subsp. capripneumoniae of different geographical origins in Africa and Asia were determined. Nucleotide differences which were present in only one of the two operons (polymorphisms) were detected in 24 positions. The polymorphisms were not randomly distributed in the 16S rRNA genes, and some of them were found in regions of low evolutionary variability. Interestingly, 11 polymorphisms were found in all the M. capricolum subsp. capripneumoniae strains, thus defining a putative ancestor. A sequence length difference between the 16S rRNA genes in a poly(A) region and 12 additional polymorphisms were found in only one or some of the strains. A phylogenetic tree was constructed by comparative analysis of the polymorphisme, and this tree revealed two distinct fines of descent. The nucleotide substitution rate of strains within line II was up to 50% higher than within line I. A tree was also constructed from individual operonal 16S rRNA sequences, and the sequences of the two operons were found to form two distinct clades. The topologies of both clades were strikingly similar, which supports the use of 16S rRNA sequence data from homologous operons for phylogenetic studies. The strain-specific polymorphism patterns of the 16S rRNA genes of M. capricolum subsp. capripneumoniae may be used as epidemiological markers for CCPP. |
format |
article |
topic_facet |
L73 - Maladies des animaux Mycoplasma capricolum épidémiologie Mycoplasma mycoides mycoplasmose arn séquence nucléotidique marqueur génétique http://aims.fao.org/aos/agrovoc/c_27242 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_23951 http://aims.fao.org/aos/agrovoc/c_5022 http://aims.fao.org/aos/agrovoc/c_6618 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_24030 |
author |
Pettersson, B. Bölske, Göran Thiaucourt, François Uhlén, M. Johansson, Karl-Erik |
author_facet |
Pettersson, B. Bölske, Göran Thiaucourt, François Uhlén, M. Johansson, Karl-Erik |
author_sort |
Pettersson, B. |
title |
Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes |
title_short |
Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes |
title_full |
Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes |
title_fullStr |
Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes |
title_full_unstemmed |
Molecular evolution of Mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16S rRNA genes |
title_sort |
molecular evolution of mycoplasma capricolum subsp. capripneumoniae strains, based on polymorphisms in the 16s rrna genes |
url |
http://agritrop.cirad.fr/390125/ http://agritrop.cirad.fr/390125/1/390125.pdf |
work_keys_str_mv |
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