Un test ELISA performant pour le diagnostic de la pleuropneumonie contagieuse bovine (CBPP)
A competitive ELISA, usina a specific monoclonal antibody, was designed to detect antibodies to Mycoplasma mycoides subsp . mycoides SC , the agent of contagi ous bovine pleuropneu mon i a. One monoclonal antibody was found suitable for such a test, '117/5', it does not cross-react with any of the other mycoplasma species tested, furthermore, its binding is inhibited by positive sera. The cutoff, 50% of inhibition, was determined usina a set of negative sera trom CBPP-free areas. The sensitivity was controlled with sera from artificially infected animals as well as from sera from areas where CBPP is enzootic. In both cases, cELISA compared favorably with CFT. The precocity of detection was similar but cELISA detected more positives and the positive titers seemed to persist longer than in the case of CFT. Lysis of the antigen used to coat the ELISA plates reduced the variability of fixation and improved the repeatability of the test. A field evaluation is now in progress which will determine the true sensitivity and specificity of the test and also check if antibodies are detected after vaccination.
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Subjects: | L73 - Maladies des animaux, péripneumonie contagieuse bovine, Test ELISA, anticorps monoclonal, Mycoplasma mycoides, http://aims.fao.org/aos/agrovoc/c_16706, http://aims.fao.org/aos/agrovoc/c_15968, http://aims.fao.org/aos/agrovoc/c_24051, http://aims.fao.org/aos/agrovoc/c_23951, |
Online Access: | http://agritrop.cirad.fr/390124/ http://agritrop.cirad.fr/390124/1/390124.pdf |
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dig-cirad-fr-3901242024-01-27T23:14:39Z http://agritrop.cirad.fr/390124/ http://agritrop.cirad.fr/390124/ Un test ELISA performant pour le diagnostic de la pleuropneumonie contagieuse bovine (CBPP). Le Goff Christian, Thiaucourt François. 1998. Veterinary Microbiology, 60 : 179-191.https://doi.org/10.1016/S0378-1135(98)00156-4 <https://doi.org/10.1016/S0378-1135(98)00156-4> Un test ELISA performant pour le diagnostic de la pleuropneumonie contagieuse bovine (CBPP) Le Goff, Christian Thiaucourt, François eng 1998 Veterinary Microbiology L73 - Maladies des animaux péripneumonie contagieuse bovine Test ELISA anticorps monoclonal Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_24051 http://aims.fao.org/aos/agrovoc/c_23951 A competitive ELISA, usina a specific monoclonal antibody, was designed to detect antibodies to Mycoplasma mycoides subsp . mycoides SC , the agent of contagi ous bovine pleuropneu mon i a. One monoclonal antibody was found suitable for such a test, '117/5', it does not cross-react with any of the other mycoplasma species tested, furthermore, its binding is inhibited by positive sera. The cutoff, 50% of inhibition, was determined usina a set of negative sera trom CBPP-free areas. The sensitivity was controlled with sera from artificially infected animals as well as from sera from areas where CBPP is enzootic. In both cases, cELISA compared favorably with CFT. The precocity of detection was similar but cELISA detected more positives and the positive titers seemed to persist longer than in the case of CFT. Lysis of the antigen used to coat the ELISA plates reduced the variability of fixation and improved the repeatability of the test. A field evaluation is now in progress which will determine the true sensitivity and specificity of the test and also check if antibodies are detected after vaccination. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/390124/1/390124.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/S0378-1135(98)00156-4 10.1016/S0378-1135(98)00156-4 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=90101 info:eu-repo/semantics/altIdentifier/doi/10.1016/S0378-1135(98)00156-4 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/S0378-1135(98)00156-4 |
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L73 - Maladies des animaux péripneumonie contagieuse bovine Test ELISA anticorps monoclonal Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_24051 http://aims.fao.org/aos/agrovoc/c_23951 L73 - Maladies des animaux péripneumonie contagieuse bovine Test ELISA anticorps monoclonal Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_24051 http://aims.fao.org/aos/agrovoc/c_23951 |
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L73 - Maladies des animaux péripneumonie contagieuse bovine Test ELISA anticorps monoclonal Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_24051 http://aims.fao.org/aos/agrovoc/c_23951 L73 - Maladies des animaux péripneumonie contagieuse bovine Test ELISA anticorps monoclonal Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_24051 http://aims.fao.org/aos/agrovoc/c_23951 Le Goff, Christian Thiaucourt, François Un test ELISA performant pour le diagnostic de la pleuropneumonie contagieuse bovine (CBPP) |
description |
A competitive ELISA, usina a specific monoclonal antibody, was designed to detect antibodies to Mycoplasma mycoides subsp . mycoides SC , the agent of contagi ous bovine pleuropneu mon i a. One monoclonal antibody was found suitable for such a test, '117/5', it does not cross-react with any of the other mycoplasma species tested, furthermore, its binding is inhibited by positive sera. The cutoff, 50% of inhibition, was determined usina a set of negative sera trom CBPP-free areas. The sensitivity was controlled with sera from artificially infected animals as well as from sera from areas where CBPP is enzootic. In both cases, cELISA compared favorably with CFT. The precocity of detection was similar but cELISA detected more positives and the positive titers seemed to persist longer than in the case of CFT. Lysis of the antigen used to coat the ELISA plates reduced the variability of fixation and improved the repeatability of the test. A field evaluation is now in progress which will determine the true sensitivity and specificity of the test and also check if antibodies are detected after vaccination. |
format |
article |
topic_facet |
L73 - Maladies des animaux péripneumonie contagieuse bovine Test ELISA anticorps monoclonal Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16706 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_24051 http://aims.fao.org/aos/agrovoc/c_23951 |
author |
Le Goff, Christian Thiaucourt, François |
author_facet |
Le Goff, Christian Thiaucourt, François |
author_sort |
Le Goff, Christian |
title |
Un test ELISA performant pour le diagnostic de la pleuropneumonie contagieuse bovine (CBPP) |
title_short |
Un test ELISA performant pour le diagnostic de la pleuropneumonie contagieuse bovine (CBPP) |
title_full |
Un test ELISA performant pour le diagnostic de la pleuropneumonie contagieuse bovine (CBPP) |
title_fullStr |
Un test ELISA performant pour le diagnostic de la pleuropneumonie contagieuse bovine (CBPP) |
title_full_unstemmed |
Un test ELISA performant pour le diagnostic de la pleuropneumonie contagieuse bovine (CBPP) |
title_sort |
un test elisa performant pour le diagnostic de la pleuropneumonie contagieuse bovine (cbpp) |
url |
http://agritrop.cirad.fr/390124/ http://agritrop.cirad.fr/390124/1/390124.pdf |
work_keys_str_mv |
AT legoffchristian untestelisaperformantpourlediagnosticdelapleuropneumoniecontagieusebovinecbpp AT thiaucourtfrancois untestelisaperformantpourlediagnosticdelapleuropneumoniecontagieusebovinecbpp |
_version_ |
1792491300520460288 |