Endogenous cytokinins as biochemical markers of rubber-tree (Hevea brasiliensis) clone rejuvenation
The endogenous levels of isopentenyladenine, isopentenyladenosine, zeatin and zeatin riboside and the ability for #in vitro# axillary shoot organogenesis and rhizogenesis were compared between mature and rejuvenated clones of #Hevea brasiliensis# (Müll. Arg.). Enhancement of the #in vitro# organogenesis ability of rubber-tree clones following somatic embryogenesis or repeated grafting onto juvenile rootstocks was accompanied by an increase of zeatin riboside levels in shoots used as starting material for #in vitro# micropropagation. Furthermore, the zeatin level, in #in vitro# shoots of clones treated by #in vitro# micrografting, and consequently capable of axillary shoot and root organogenesis, was higher than in #in vitro# shoots of non treated mature material incapable of #in vitro# organogenesis. We conclude that the endogenous zeatin-like cytokinin level (free and ribosylated forms) can be considered as a reliable marker for the recovery of #in vitro# shoot and root organogenesis after rejuvenating treatments in rubber-tree clones.
| Main Authors: | , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | F30 - Génétique et amélioration des plantes, F02 - Multiplication végétative des plantes, Hevea brasiliensis, clone, cytokinine, zéatine, marqueur génétique, stade de développement, développement embryonnaire, organogénèse, culture in vitro, micropropagation, Embryogénèse somatique, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_1678, http://aims.fao.org/aos/agrovoc/c_2090, http://aims.fao.org/aos/agrovoc/c_8505, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_2230, http://aims.fao.org/aos/agrovoc/c_2545, http://aims.fao.org/aos/agrovoc/c_27791, http://aims.fao.org/aos/agrovoc/c_24135, http://aims.fao.org/aos/agrovoc/c_24136, http://aims.fao.org/aos/agrovoc/c_36911, |
| Online Access: | http://agritrop.cirad.fr/390062/ http://agritrop.cirad.fr/390062/1/390062.pdf |
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