Somatic embryogenesis in plantain banana

A cell suspension of French Sombre plantain banane (#Musa# spp. AAB genome) was initiated from callus obtained from young male flowers. Histological examination enabled us to describe and follow the evolution of the suspension consisting of: embryogenic aggregates, proembryos, nodules, and isolated cells. It demonstrated the unicellular origin of somatic embryos, either during maintenance of the suspension or after plating on a semisolid medium. The cells from which the embryos originated had no starch but only protein reserves. Plating 1 ml of packed cells from the suspension led to the formation of "10 to the power of 5" embryos of which 10 to 40% could be converted into plantlets.

Bibliographic Details

Main Authors:	Grapin, Agnès, Schwendiman, Jacques, Teisson, Claude
Format:	article biblioteca
Language:	eng
Subjects:	F62 - Physiologie végétale - Croissance et développement, Musa, culture in vitro, Embryogénèse somatique, analyse histocytologique, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_24135, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_27541,
Online Access:	http://agritrop.cirad.fr/389845/
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