Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars
Increasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 historical wheat cultivars of Pakistan under high temperature at seedling stage to identify loci associated with heat tolerance using genome-wide association studies (GWAS). A quantitative trait locus, TaHST1, on chr4A was also characterized to identify the haplotypes at this locus associated with heat tolerance in wheat from Pakistan. Initially, the diversity panel was planted under control conditions (25°C/20°C day and night temperature) in a glass house. At three leaf stage, plants were subjected to heat stress (HS) by increasing temperature (40°C/35°C day and night), while one treatment was kept at control condition. After 7 days of HS, data were collected for seedling morphology. Heat stress reduced these traits by 25% (root weight) to 40% (shoot weight), and shoot biomass was largely affected by heat stress. A GWAS model, fixed and random model circulating probability unification (FarmCPU), identified 43 quantitative trait nucleotides (QTNs) on all chromosomes, except chr7B, were associated under both HS and control conditions. Thirteen QTNs were identified in control, while 30 QTNs were identified in HS condition. In total, 24 haplotypes were identified at TaHST1 locus, and most of the heat tolerant genotypes were assigned to Hap-20 and Hap-21. Eleven QTNs were identified within 0.3–3.1 Mb proximity of heat shock protein (HSP). Conclusively, this study provided a detailed genetic framework of heat tolerance in wheat at the seedling stage and identify potential genetic regions associated with heat tolerance which can be used for marker assisted selection (MAS) in breeding for heat stress tolerance.
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Language: | English |
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Frontiers
2022
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Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Heat Stress Tolerance, Quantitative Trait Nucleotides, Single Nucleotide Polymorphism Markers, HEAT STRESS, QUANTITATIVE TRAIT LOCI, NUCLEOTIDES, SINGLE NUCLEOTIDE POLYMORPHISM, SPRING WHEAT, Wheat, |
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dig-cimmyt-10883-224642024-02-22T14:21:59Z Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars Muhammad Ibrar Khan Kainat, Z. Maqbool, S. Mehwish, A. Ahmad, S. Hafiz Muhammad Suleman Mahmood, Z. Ali, M. Aziz, A. Awais Rasheed Huihui Li AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Heat Stress Tolerance Quantitative Trait Nucleotides Single Nucleotide Polymorphism Markers HEAT STRESS QUANTITATIVE TRAIT LOCI NUCLEOTIDES SINGLE NUCLEOTIDE POLYMORPHISM SPRING WHEAT Wheat Increasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 historical wheat cultivars of Pakistan under high temperature at seedling stage to identify loci associated with heat tolerance using genome-wide association studies (GWAS). A quantitative trait locus, TaHST1, on chr4A was also characterized to identify the haplotypes at this locus associated with heat tolerance in wheat from Pakistan. Initially, the diversity panel was planted under control conditions (25°C/20°C day and night temperature) in a glass house. At three leaf stage, plants were subjected to heat stress (HS) by increasing temperature (40°C/35°C day and night), while one treatment was kept at control condition. After 7 days of HS, data were collected for seedling morphology. Heat stress reduced these traits by 25% (root weight) to 40% (shoot weight), and shoot biomass was largely affected by heat stress. A GWAS model, fixed and random model circulating probability unification (FarmCPU), identified 43 quantitative trait nucleotides (QTNs) on all chromosomes, except chr7B, were associated under both HS and control conditions. Thirteen QTNs were identified in control, while 30 QTNs were identified in HS condition. In total, 24 haplotypes were identified at TaHST1 locus, and most of the heat tolerant genotypes were assigned to Hap-20 and Hap-21. Eleven QTNs were identified within 0.3–3.1 Mb proximity of heat shock protein (HSP). Conclusively, this study provided a detailed genetic framework of heat tolerance in wheat at the seedling stage and identify potential genetic regions associated with heat tolerance which can be used for marker assisted selection (MAS) in breeding for heat stress tolerance. 2023-01-25T01:20:24Z 2023-01-25T01:20:24Z 2022 Article Published Version https://hdl.handle.net/10883/22464 10.3389/fpls.2022.972481 English https://figshare.com/collections/Genome-wide_association_for_heat_tolerance_at_seedling_stage_in_historical_spring_wheat_cultivars/6165252 Climate adaptation & mitigation Nutrition, health & food security Accelerated Breeding Genetic Innovation National Science Foundation of China Project of Hainan Yazhou Bay Seed Laboratory CGIAR Trust Fund https://hdl.handle.net/10568/128239 CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose Open Access Switzerland Frontiers 13 1664-462X Frontiers in Plant Science 972481 |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Heat Stress Tolerance Quantitative Trait Nucleotides Single Nucleotide Polymorphism Markers HEAT STRESS QUANTITATIVE TRAIT LOCI NUCLEOTIDES SINGLE NUCLEOTIDE POLYMORPHISM SPRING WHEAT Wheat AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Heat Stress Tolerance Quantitative Trait Nucleotides Single Nucleotide Polymorphism Markers HEAT STRESS QUANTITATIVE TRAIT LOCI NUCLEOTIDES SINGLE NUCLEOTIDE POLYMORPHISM SPRING WHEAT Wheat |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Heat Stress Tolerance Quantitative Trait Nucleotides Single Nucleotide Polymorphism Markers HEAT STRESS QUANTITATIVE TRAIT LOCI NUCLEOTIDES SINGLE NUCLEOTIDE POLYMORPHISM SPRING WHEAT Wheat AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Heat Stress Tolerance Quantitative Trait Nucleotides Single Nucleotide Polymorphism Markers HEAT STRESS QUANTITATIVE TRAIT LOCI NUCLEOTIDES SINGLE NUCLEOTIDE POLYMORPHISM SPRING WHEAT Wheat Muhammad Ibrar Khan Kainat, Z. Maqbool, S. Mehwish, A. Ahmad, S. Hafiz Muhammad Suleman Mahmood, Z. Ali, M. Aziz, A. Awais Rasheed Huihui Li Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
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Increasing global temperature has adverse effects on crop health and productivity at both seedling and reproductivity stages. It is paramount to develop heat tolerant wheat cultivars able to sustain under high and fluctuating temperature conditions. An experiment was conducted to characterize 194 historical wheat cultivars of Pakistan under high temperature at seedling stage to identify loci associated with heat tolerance using genome-wide association studies (GWAS). A quantitative trait locus, TaHST1, on chr4A was also characterized to identify the haplotypes at this locus associated with heat tolerance in wheat from Pakistan. Initially, the diversity panel was planted under control conditions (25°C/20°C day and night temperature) in a glass house. At three leaf stage, plants were subjected to heat stress (HS) by increasing temperature (40°C/35°C day and night), while one treatment was kept at control condition. After 7 days of HS, data were collected for seedling morphology. Heat stress reduced these traits by 25% (root weight) to 40% (shoot weight), and shoot biomass was largely affected by heat stress. A GWAS model, fixed and random model circulating probability unification (FarmCPU), identified 43 quantitative trait nucleotides (QTNs) on all chromosomes, except chr7B, were associated under both HS and control conditions. Thirteen QTNs were identified in control, while 30 QTNs were identified in HS condition. In total, 24 haplotypes were identified at TaHST1 locus, and most of the heat tolerant genotypes were assigned to Hap-20 and Hap-21. Eleven QTNs were identified within 0.3–3.1 Mb proximity of heat shock protein (HSP). Conclusively, this study provided a detailed genetic framework of heat tolerance in wheat at the seedling stage and identify potential genetic regions associated with heat tolerance which can be used for marker assisted selection (MAS) in breeding for heat stress tolerance. |
format |
Article |
topic_facet |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Heat Stress Tolerance Quantitative Trait Nucleotides Single Nucleotide Polymorphism Markers HEAT STRESS QUANTITATIVE TRAIT LOCI NUCLEOTIDES SINGLE NUCLEOTIDE POLYMORPHISM SPRING WHEAT Wheat |
author |
Muhammad Ibrar Khan Kainat, Z. Maqbool, S. Mehwish, A. Ahmad, S. Hafiz Muhammad Suleman Mahmood, Z. Ali, M. Aziz, A. Awais Rasheed Huihui Li |
author_facet |
Muhammad Ibrar Khan Kainat, Z. Maqbool, S. Mehwish, A. Ahmad, S. Hafiz Muhammad Suleman Mahmood, Z. Ali, M. Aziz, A. Awais Rasheed Huihui Li |
author_sort |
Muhammad Ibrar Khan |
title |
Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
title_short |
Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
title_full |
Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
title_fullStr |
Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
title_full_unstemmed |
Genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
title_sort |
genome-wide association for heat tolerance at seedling stage in historical spring wheat cultivars |
publisher |
Frontiers |
publishDate |
2022 |
url |
https://hdl.handle.net/10883/22464 |
work_keys_str_mv |
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1792501491557203968 |