Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa
Chickpea (Cicer arietinum L.) is an important legume crop in the semi-arid regions of Asia and Africa. Gains in crop productivity have been low however, particularly because of biotic and abiotic stresses. To help enhance crop productivity using molecular breeding techniques, next generation sequencing technologies such as Roche/454 and Illumina/Solexa were used to determine the sequence of most gene transcripts and to identify drought-responsive genes and gene-based molecular markers. A total of 103215 tentative unique sequences (TUSs) have been produced from 435018 Roche/454 reads and 21491 Sanger expressed sequence tags (ESTs). Putative functions were determined for 49437 (47.8%) of the TUSs, and gene ontology assignments were determined for 20634 (41.7%) of the TUSs. Comparison of the chickpea TUSs with the Medicago truncatula genome assembly (Mt 3.5.1 build) resulted in 42141 aligned TUSs with putative gene structures (including 39281 predicted intron/splice junctions). Alignment of ∼37 million Illumina/Solexa tags generated from drought-challenged root tissues of two chickpea genotypes against the TUSs identified 44639 differentially expressed TUSs. The TUSs were also used to identify a diverse set of markers, including 728 simple sequence repeats (SSRs), 495 single nucleotide polymorphisms (SNPs), 387 conserved orthologous sequence (COS) markers, and 2088 intron-spanning region (ISR) markers. This resource will be useful for basic and applied research for genome analysis and crop improvement in chickpea.
Main Authors: | , , , , , , , , , , , , , , , , , , , , |
---|---|
Format: | Article biblioteca |
Language: | English |
Published: |
Wiley
2011
|
Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Next Generation Sequencing, Drought-Responsive Genes, CHICKPEAS, TRANSCRIPTION, GENES, DROUGHT TOLERANCE, GENETIC MARKERS, |
Online Access: | https://hdl.handle.net/10883/21094 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cimmyt-10883-21094 |
---|---|
record_format |
koha |
spelling |
dig-cimmyt-10883-210942022-08-31T19:11:57Z Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa Hiremath, P.J. Farmer, A. Cannon, S.B. Woodward, J. Kudapa, H. Tuteja, R. Kumar, A. Bhanuprakash, A. Mulaosmanovic, B. Gujaria, N. Krishnamurthy, L. Pooran M. Gaur Kavikishor, P.B. Shah, T. Srinivasan, R. Lohse, M. Yongli Xiao Town, C.D. Cook, D. May, G.D. Varshney, R.K. AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Next Generation Sequencing Drought-Responsive Genes CHICKPEAS TRANSCRIPTION GENES DROUGHT TOLERANCE GENETIC MARKERS Chickpea (Cicer arietinum L.) is an important legume crop in the semi-arid regions of Asia and Africa. Gains in crop productivity have been low however, particularly because of biotic and abiotic stresses. To help enhance crop productivity using molecular breeding techniques, next generation sequencing technologies such as Roche/454 and Illumina/Solexa were used to determine the sequence of most gene transcripts and to identify drought-responsive genes and gene-based molecular markers. A total of 103215 tentative unique sequences (TUSs) have been produced from 435018 Roche/454 reads and 21491 Sanger expressed sequence tags (ESTs). Putative functions were determined for 49437 (47.8%) of the TUSs, and gene ontology assignments were determined for 20634 (41.7%) of the TUSs. Comparison of the chickpea TUSs with the Medicago truncatula genome assembly (Mt 3.5.1 build) resulted in 42141 aligned TUSs with putative gene structures (including 39281 predicted intron/splice junctions). Alignment of ∼37 million Illumina/Solexa tags generated from drought-challenged root tissues of two chickpea genotypes against the TUSs identified 44639 differentially expressed TUSs. The TUSs were also used to identify a diverse set of markers, including 728 simple sequence repeats (SSRs), 495 single nucleotide polymorphisms (SNPs), 387 conserved orthologous sequence (COS) markers, and 2088 intron-spanning region (ISR) markers. This resource will be useful for basic and applied research for genome analysis and crop improvement in chickpea. 922-931 2021-01-12T01:10:15Z 2021-01-12T01:10:15Z 2011 Article Published Version https://hdl.handle.net/10883/21094 10.1111/j.1467-7652.2011.00625.x English CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose Open Access Oxford (United Kingdom) Wiley 8 9 1467-7644 Plant Biotechnology Journal |
institution |
CIMMYT |
collection |
DSpace |
country |
México |
countrycode |
MX |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cimmyt |
tag |
biblioteca |
region |
America del Norte |
libraryname |
CIMMYT Library |
language |
English |
topic |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Next Generation Sequencing Drought-Responsive Genes CHICKPEAS TRANSCRIPTION GENES DROUGHT TOLERANCE GENETIC MARKERS AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Next Generation Sequencing Drought-Responsive Genes CHICKPEAS TRANSCRIPTION GENES DROUGHT TOLERANCE GENETIC MARKERS |
spellingShingle |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Next Generation Sequencing Drought-Responsive Genes CHICKPEAS TRANSCRIPTION GENES DROUGHT TOLERANCE GENETIC MARKERS AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Next Generation Sequencing Drought-Responsive Genes CHICKPEAS TRANSCRIPTION GENES DROUGHT TOLERANCE GENETIC MARKERS Hiremath, P.J. Farmer, A. Cannon, S.B. Woodward, J. Kudapa, H. Tuteja, R. Kumar, A. Bhanuprakash, A. Mulaosmanovic, B. Gujaria, N. Krishnamurthy, L. Pooran M. Gaur Kavikishor, P.B. Shah, T. Srinivasan, R. Lohse, M. Yongli Xiao Town, C.D. Cook, D. May, G.D. Varshney, R.K. Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa |
description |
Chickpea (Cicer arietinum L.) is an important legume crop in the semi-arid regions of Asia and Africa. Gains in crop productivity have been low however, particularly because of biotic and abiotic stresses. To help enhance crop productivity using molecular breeding techniques, next generation sequencing technologies such as Roche/454 and Illumina/Solexa were used to determine the sequence of most gene transcripts and to identify drought-responsive genes and gene-based molecular markers. A total of 103215 tentative unique sequences (TUSs) have been produced from 435018 Roche/454 reads and 21491 Sanger expressed sequence tags (ESTs). Putative functions were determined for 49437 (47.8%) of the TUSs, and gene ontology assignments were determined for 20634 (41.7%) of the TUSs. Comparison of the chickpea TUSs with the Medicago truncatula genome assembly (Mt 3.5.1 build) resulted in 42141 aligned TUSs with putative gene structures (including 39281 predicted intron/splice junctions). Alignment of ∼37 million Illumina/Solexa tags generated from drought-challenged root tissues of two chickpea genotypes against the TUSs identified 44639 differentially expressed TUSs. The TUSs were also used to identify a diverse set of markers, including 728 simple sequence repeats (SSRs), 495 single nucleotide polymorphisms (SNPs), 387 conserved orthologous sequence (COS) markers, and 2088 intron-spanning region (ISR) markers. This resource will be useful for basic and applied research for genome analysis and crop improvement in chickpea. |
format |
Article |
topic_facet |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Next Generation Sequencing Drought-Responsive Genes CHICKPEAS TRANSCRIPTION GENES DROUGHT TOLERANCE GENETIC MARKERS |
author |
Hiremath, P.J. Farmer, A. Cannon, S.B. Woodward, J. Kudapa, H. Tuteja, R. Kumar, A. Bhanuprakash, A. Mulaosmanovic, B. Gujaria, N. Krishnamurthy, L. Pooran M. Gaur Kavikishor, P.B. Shah, T. Srinivasan, R. Lohse, M. Yongli Xiao Town, C.D. Cook, D. May, G.D. Varshney, R.K. |
author_facet |
Hiremath, P.J. Farmer, A. Cannon, S.B. Woodward, J. Kudapa, H. Tuteja, R. Kumar, A. Bhanuprakash, A. Mulaosmanovic, B. Gujaria, N. Krishnamurthy, L. Pooran M. Gaur Kavikishor, P.B. Shah, T. Srinivasan, R. Lohse, M. Yongli Xiao Town, C.D. Cook, D. May, G.D. Varshney, R.K. |
author_sort |
Hiremath, P.J. |
title |
Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa |
title_short |
Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa |
title_full |
Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa |
title_fullStr |
Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa |
title_full_unstemmed |
Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa |
title_sort |
large-scale transcriptome analysis in chickpea (cicer arietinum l.), an orphan legume crop of the semi-arid tropics of asia and africa |
publisher |
Wiley |
publishDate |
2011 |
url |
https://hdl.handle.net/10883/21094 |
work_keys_str_mv |
AT hiremathpj largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT farmera largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT cannonsb largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT woodwardj largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT kudapah largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT tutejar largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT kumara largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT bhanuprakasha largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT mulaosmanovicb largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT gujarian largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT krishnamurthyl largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT pooranmgaur largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT kavikishorpb largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT shaht largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT srinivasanr largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT lohsem largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT yonglixiao largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT towncd largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT cookd largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT maygd largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica AT varshneyrk largescaletranscriptomeanalysisinchickpeacicerarietinumlanorphanlegumecropofthesemiaridtropicsofasiaandafrica |
_version_ |
1756086939961786368 |