Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines

Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines

Developing wheat–alien chromosome introgression lines to improve bread wheat’s resistance to stresses, such as drought, salinity stress and diseases, requires reliable markers to identify and characterize the alien chromatins. Leymus mollis is a wild relative of bread wheat resistant to salinity and economically important diseases of wheat, but its genome sequence and cytological markers are not available. We devised a molecular marker-assisted strategy for L. mollis chromosome identification and applied it to produce 10 wheat–L. mollis chromosome addition lines. Using 47L. racemosus genome polymorphic PCR markers and DArTseq genotyping, we distinguished the L. mollis chromosomes and differentiated disomic and monosomic lines by progeny test. DArTseq genotyping generated 14,530L. mollis SNP markers and the chromosome-specifc SNP markers were used to determine the homoeologous groups of L. mollis chromosomes in the addition lines. To validate the marker-based results, genomic in situ hybridization was applied to confirm the presence and cytological status of L. mollis chromosomes in the lines. This study demonstrates that adequate molecular markers allow the production and characterization of wheat–alien addition lines without in situ hybridization, which saves considerable time and effort.

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Main Authors: Edet, O.U., Gorafi, Y.S.A, Seong-woo Cho, Kishii, M., Hisashi Tsujimoto
Format: Article biblioteca
Language:English
Published: Springer Nature 2018
Subjects:AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Genetic Hybridization, GENETIC MARKERS, PLANT BREEDING,
Online Access:https://hdl.handle.net/10883/19824
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spelling dig-cimmyt-10883-198242021-07-06T14:10:04Z Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines Edet, O.U. Gorafi, Y.S.A Seong-woo Cho Kishii, M. Hisashi Tsujimoto AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Genetic Hybridization GENETIC MARKERS PLANT BREEDING Developing wheat–alien chromosome introgression lines to improve bread wheat’s resistance to stresses, such as drought, salinity stress and diseases, requires reliable markers to identify and characterize the alien chromatins. Leymus mollis is a wild relative of bread wheat resistant to salinity and economically important diseases of wheat, but its genome sequence and cytological markers are not available. We devised a molecular marker-assisted strategy for L. mollis chromosome identification and applied it to produce 10 wheat–L. mollis chromosome addition lines. Using 47L. racemosus genome polymorphic PCR markers and DArTseq genotyping, we distinguished the L. mollis chromosomes and differentiated disomic and monosomic lines by progeny test. DArTseq genotyping generated 14,530L. mollis SNP markers and the chromosome-specifc SNP markers were used to determine the homoeologous groups of L. mollis chromosomes in the addition lines. To validate the marker-based results, genomic in situ hybridization was applied to confirm the presence and cytological status of L. mollis chromosomes in the lines. This study demonstrates that adequate molecular markers allow the production and characterization of wheat–alien addition lines without in situ hybridization, which saves considerable time and effort. 2019-01-17T23:32:35Z 2019-01-17T23:32:35Z 2018 Article ESSN: 2045-2322 https://hdl.handle.net/10883/19824 10.1038/s41598-018-34545-x English CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose. Open Access PDF United Kingdom Springer Nature art. 16117 8 Scientific Reports
institution CIMMYT
collection DSpace
country México
countrycode MX
component Bibliográfico
access En linea
databasecode dig-cimmyt
tag biblioteca
region America del Norte
libraryname CIMMYT Library
language English
topic AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Genetic Hybridization
GENETIC MARKERS
PLANT BREEDING
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Genetic Hybridization
GENETIC MARKERS
PLANT BREEDING
spellingShingle AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Genetic Hybridization
GENETIC MARKERS
PLANT BREEDING
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Genetic Hybridization
GENETIC MARKERS
PLANT BREEDING
Edet, O.U.
Gorafi, Y.S.A
Seong-woo Cho
Kishii, M.
Hisashi Tsujimoto
Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines
description Developing wheat–alien chromosome introgression lines to improve bread wheat’s resistance to stresses, such as drought, salinity stress and diseases, requires reliable markers to identify and characterize the alien chromatins. Leymus mollis is a wild relative of bread wheat resistant to salinity and economically important diseases of wheat, but its genome sequence and cytological markers are not available. We devised a molecular marker-assisted strategy for L. mollis chromosome identification and applied it to produce 10 wheat–L. mollis chromosome addition lines. Using 47L. racemosus genome polymorphic PCR markers and DArTseq genotyping, we distinguished the L. mollis chromosomes and differentiated disomic and monosomic lines by progeny test. DArTseq genotyping generated 14,530L. mollis SNP markers and the chromosome-specifc SNP markers were used to determine the homoeologous groups of L. mollis chromosomes in the addition lines. To validate the marker-based results, genomic in situ hybridization was applied to confirm the presence and cytological status of L. mollis chromosomes in the lines. This study demonstrates that adequate molecular markers allow the production and characterization of wheat–alien addition lines without in situ hybridization, which saves considerable time and effort.
format Article
topic_facet AGRICULTURAL SCIENCES AND BIOTECHNOLOGY
Genetic Hybridization
GENETIC MARKERS
PLANT BREEDING
author Edet, O.U.
Gorafi, Y.S.A
Seong-woo Cho
Kishii, M.
Hisashi Tsujimoto
author_facet Edet, O.U.
Gorafi, Y.S.A
Seong-woo Cho
Kishii, M.
Hisashi Tsujimoto
author_sort Edet, O.U.
title Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines
title_short Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines
title_full Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines
title_fullStr Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines
title_full_unstemmed Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines
title_sort novel molecular marker-assisted strategy for production of wheat-leymus mollis chromosome addition lines
publisher Springer Nature
publishDate 2018
url https://hdl.handle.net/10883/19824
work_keys_str_mv AT edetou novelmolecularmarkerassistedstrategyforproductionofwheatleymusmollischromosomeadditionlines
AT gorafiysa novelmolecularmarkerassistedstrategyforproductionofwheatleymusmollischromosomeadditionlines
AT seongwoocho novelmolecularmarkerassistedstrategyforproductionofwheatleymusmollischromosomeadditionlines
AT kishiim novelmolecularmarkerassistedstrategyforproductionofwheatleymusmollischromosomeadditionlines
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