A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes
Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples
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2014
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Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Dengue Virus, Microarrays, VECTOR-BORNE DISEASES, HUMANS, AEDES, VIRUSES, |
Online Access: | https://hdl.handle.net/10883/19772 |
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dig-cimmyt-10883-197722021-05-13T16:12:24Z A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes Díaz-Badillo, A. Lourdes Muñoz, M. de Perez-Ramirez, G. Altuzar, V. Burgueño, J. Mendoza-Alvarez, J.G. Martínez-Muñoz, J.P. Cisneros, A. Navarrete-Espinosa, J. Sanchez-Sinencio, F. A AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Dengue Virus Microarrays VECTOR-BORNE DISEASES HUMANS AEDES VIRUSES Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples 7580-7601 2019-01-11T20:48:51Z 2019-01-11T20:48:51Z 2014 Article 1424-8220 1424-8220 https://hdl.handle.net/10883/19772 10.3390/s140507580 English CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose. Open Access PDF Switzerland MDPI 5 14 Sensors |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Dengue Virus Microarrays VECTOR-BORNE DISEASES HUMANS AEDES VIRUSES AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Dengue Virus Microarrays VECTOR-BORNE DISEASES HUMANS AEDES VIRUSES |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Dengue Virus Microarrays VECTOR-BORNE DISEASES HUMANS AEDES VIRUSES AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Dengue Virus Microarrays VECTOR-BORNE DISEASES HUMANS AEDES VIRUSES Díaz-Badillo, A. Lourdes Muñoz, M. de Perez-Ramirez, G. Altuzar, V. Burgueño, J. Mendoza-Alvarez, J.G. Martínez-Muñoz, J.P. Cisneros, A. Navarrete-Espinosa, J. Sanchez-Sinencio, F. A A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes |
description |
Here; we have described and tested a microarray based-method for the screening of dengue virus (DENV) serotypes. This DNA microarray assay is specific and sensitive and can detect dual infections with two dengue virus serotypes and single-serotype infections. Other methodologies may underestimate samples containing more than one serotype. This technology can be used to discriminate between the four DENV serotypes. Single-stranded DNA targets were covalently attached to glass slides and hybridised with specific labelled probes. DENV isolates and dengue samples were used to evaluate microarray performance. Our results demonstrate that the probes hybridized specifically to DENV serotypes; with no detection of unspecific signals. This finding provides evidence that specific probes can effectively identify single and double infections in DENV samples |
format |
Article |
topic_facet |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Dengue Virus Microarrays VECTOR-BORNE DISEASES HUMANS AEDES VIRUSES |
author |
Díaz-Badillo, A. Lourdes Muñoz, M. de Perez-Ramirez, G. Altuzar, V. Burgueño, J. Mendoza-Alvarez, J.G. Martínez-Muñoz, J.P. Cisneros, A. Navarrete-Espinosa, J. Sanchez-Sinencio, F. A |
author_facet |
Díaz-Badillo, A. Lourdes Muñoz, M. de Perez-Ramirez, G. Altuzar, V. Burgueño, J. Mendoza-Alvarez, J.G. Martínez-Muñoz, J.P. Cisneros, A. Navarrete-Espinosa, J. Sanchez-Sinencio, F. A |
author_sort |
Díaz-Badillo, A. |
title |
A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes |
title_short |
A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes |
title_full |
A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes |
title_fullStr |
A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes |
title_full_unstemmed |
A DNA Microarray-based assay to detect dual infection with two dengue virus serotypes |
title_sort |
dna microarray-based assay to detect dual infection with two dengue virus serotypes |
publisher |
MDPI |
publishDate |
2014 |
url |
https://hdl.handle.net/10883/19772 |
work_keys_str_mv |
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