Analysis of the masked metabolite of deoxynivalenol and Fusarium resistance in CIMMYT wheat germplasm

Fusarium head blight (FHB) causes significant grain loss and contamination of grains with harmful mycotoxins, especially deoxynivalenol (DON). Fusarium resistance and DON accumulation have been extensively investigated in various cultivars; however, the level of DON-3-O-glucoside (D3G) has not been as carefully studied. In this study, we measured accumulated DON and D3G levels in CIMMYT wheat elite germplasm using an analytical method validated in-house. Co-occurring nivalenol (NIV) and ergostrerol (ERG) were also analyzed. LC-MS/MS and LC-UV analyses were applied to the 50 CIMMYT elite wheat lines. D3G showed rather high correlation with DON (r = 0.82), while FHB symptoms showed slight correlation with DON and D3G (r = 0.36 and 0.32, respectively). D3G/DON ratio varied widely from 8.1 to 37.7%, and the ratio was not related with FHB resistance in this dataset.

Bibliographic Details

Main Authors:	Nakagawa, H., Xinyao He, Yosuke Matsuo, Singh, P.K., Masayo Kushiro
Format:	Article biblioteca
Language:	English
Published:	MDPI 2017
Subjects:	AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Fusarium Head Blight, Deoxynivalenol, Deoxynivalenol-3-O-Glucoside, High Performance Liquid Chromatography With Tandem Mass Spectrometry (LC-MS/MS), FUSARIUM, BLIGHT, TOXINS, HPLC, MASS SPECTROMETRY,
Online Access:	http://hdl.handle.net/10883/18882
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