Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima)
Several lowland NERICAs (New Rice for Africa) were derived from crosses between IR64 (an Oryza sativa subsp. indica variety) and Tog5681 (an Oryza glaberrima variety) that possess useful traits adapted to lowland conditions in West Africa. The proportion of parental genomic contribution and extent of genetic differences among these sister lines is unknown at the molecular level. The objectives in this study were therefore to determine, with 60 SSR markers that cover 1162 cM of the rice genome, the frequency and magnitude of deviations from the expected parental contributions among 21 BC2F10, 17 BC3 F8 and 10 BC4F8 lines and determine patterns of their genetic relationships. The estimated average O. glaberrima genome coverage was 7.2% (83.5 cM) at BC2F10, 8.5% (99.3 cM) at BC3F8 and 8.1% (93.8 cM) at BC4F8 lines. The O. sativa parent accounted for 73.2% (851.3 cM) at BC2F10, 82.6% (959.5 cM) at BC3F8 and 78.2% (908.6 cM) at BC3F8. Non-parental alleles were detected at all 3 backcross generations but the frequency of such alleles at BC2 (8.8%) was twice that of BC3F8 (3.4%) and nine times that of BC4F8 (0.9%). Both cluster and principal component analyses revealed two major groups irrespective of the level of backcross generations and the proportion of parental genome contribution.
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Academic Journals
2008
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Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, New Rice for Africa, NERICA, RICE, INTROGRESSION, MICROSATELLITES, ORYZA GLABERRIMA, RESEARCH INSTITUTIONS, |
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dig-cimmyt-10883-17552023-10-19T17:50:23Z Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima) Ndjiondjop, M.N. Fentaye Kassa Semagn Sié, M. Cissoko, M. Fatondji, B. Jones, M. AGRICULTURAL SCIENCES AND BIOTECHNOLOGY New Rice for Africa NERICA RICE INTROGRESSION MICROSATELLITES ORYZA GLABERRIMA RESEARCH INSTITUTIONS Several lowland NERICAs (New Rice for Africa) were derived from crosses between IR64 (an Oryza sativa subsp. indica variety) and Tog5681 (an Oryza glaberrima variety) that possess useful traits adapted to lowland conditions in West Africa. The proportion of parental genomic contribution and extent of genetic differences among these sister lines is unknown at the molecular level. The objectives in this study were therefore to determine, with 60 SSR markers that cover 1162 cM of the rice genome, the frequency and magnitude of deviations from the expected parental contributions among 21 BC2F10, 17 BC3 F8 and 10 BC4F8 lines and determine patterns of their genetic relationships. The estimated average O. glaberrima genome coverage was 7.2% (83.5 cM) at BC2F10, 8.5% (99.3 cM) at BC3F8 and 8.1% (93.8 cM) at BC4F8 lines. The O. sativa parent accounted for 73.2% (851.3 cM) at BC2F10, 82.6% (959.5 cM) at BC3F8 and 78.2% (908.6 cM) at BC3F8. Non-parental alleles were detected at all 3 backcross generations but the frequency of such alleles at BC2 (8.8%) was twice that of BC3F8 (3.4%) and nine times that of BC4F8 (0.9%). Both cluster and principal component analyses revealed two major groups irrespective of the level of backcross generations and the proportion of parental genome contribution. 4219-4229 2013-06-07T21:02:35Z 2013-06-07T21:02:35Z 2008 Article http://hdl.handle.net/10883/1755 English Open Access PDF Academic Journals https://academicjournals.org/journal/AJB/article-abstract/CB07F137838 23 7 African Journal of Biotechnology |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY New Rice for Africa NERICA RICE INTROGRESSION MICROSATELLITES ORYZA GLABERRIMA RESEARCH INSTITUTIONS AGRICULTURAL SCIENCES AND BIOTECHNOLOGY New Rice for Africa NERICA RICE INTROGRESSION MICROSATELLITES ORYZA GLABERRIMA RESEARCH INSTITUTIONS |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY New Rice for Africa NERICA RICE INTROGRESSION MICROSATELLITES ORYZA GLABERRIMA RESEARCH INSTITUTIONS AGRICULTURAL SCIENCES AND BIOTECHNOLOGY New Rice for Africa NERICA RICE INTROGRESSION MICROSATELLITES ORYZA GLABERRIMA RESEARCH INSTITUTIONS Ndjiondjop, M.N. Fentaye Kassa Semagn Sié, M. Cissoko, M. Fatondji, B. Jones, M. Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima) |
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Several lowland NERICAs (New Rice for Africa) were derived from crosses between IR64 (an Oryza sativa subsp. indica variety) and Tog5681 (an Oryza glaberrima variety) that possess useful traits adapted to lowland conditions in West Africa. The proportion of parental genomic contribution and extent of genetic differences among these sister lines is unknown at the molecular level. The objectives in this study were therefore to determine, with 60 SSR markers that cover 1162 cM of the rice genome, the frequency and magnitude of deviations from the expected parental contributions among 21 BC2F10, 17 BC3 F8 and 10 BC4F8 lines and determine patterns of their genetic relationships. The estimated average O. glaberrima genome coverage was 7.2% (83.5 cM) at BC2F10, 8.5% (99.3 cM) at BC3F8 and 8.1% (93.8 cM) at BC4F8 lines. The O. sativa parent accounted for 73.2% (851.3 cM) at BC2F10, 82.6% (959.5 cM) at BC3F8 and 78.2% (908.6 cM) at BC3F8. Non-parental alleles were detected at all 3 backcross generations but the frequency of such alleles at BC2 (8.8%) was twice that of BC3F8 (3.4%) and nine times that of BC4F8 (0.9%). Both cluster and principal component analyses revealed two major groups irrespective of the level of backcross generations and the proportion of parental genome contribution. |
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Article |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY New Rice for Africa NERICA RICE INTROGRESSION MICROSATELLITES ORYZA GLABERRIMA RESEARCH INSTITUTIONS |
author |
Ndjiondjop, M.N. Fentaye Kassa Semagn Sié, M. Cissoko, M. Fatondji, B. Jones, M. |
author_facet |
Ndjiondjop, M.N. Fentaye Kassa Semagn Sié, M. Cissoko, M. Fatondji, B. Jones, M. |
author_sort |
Ndjiondjop, M.N. |
title |
Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima) |
title_short |
Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima) |
title_full |
Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima) |
title_fullStr |
Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima) |
title_full_unstemmed |
Molecular profiling of interspecific lowland rice populations derived from IR64 (Oryza sativa) and Tog5681 (Oryza glaberrima) |
title_sort |
molecular profiling of interspecific lowland rice populations derived from ir64 (oryza sativa) and tog5681 (oryza glaberrima) |
publisher |
Academic Journals |
publishDate |
2008 |
url |
http://hdl.handle.net/10883/1755 |
work_keys_str_mv |
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