Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries

Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries

5 paginas, 2 tablas.

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Bibliographic Details
Main Authors: Segarra-Moragues, José G., Gleiser, G.
Other Authors: Generalitat Valenciana
Format: artículo biblioteca
Language:English
Published: Springer 2009-06
Subjects:Labiatae, Rosmarinus, Genetic diversity, SSR,
Online Access:http://hdl.handle.net/10261/58980
http://dx.doi.org/10.13039/501100003359
http://dx.doi.org/10.13039/501100003339
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spelling dig-cide-es-10261-589802016-05-13T11:08:09Z Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries Segarra-Moragues, José G. Gleiser, G. Generalitat Valenciana Consejo Superior de Investigaciones Científicas (España) Ministerio de Educación y Ciencia (España) Labiatae Rosmarinus Genetic diversity SSR 5 paginas, 2 tablas. An enrichment protocol was used to isolate and characterise microsatellite loci in Rosmarinus officinalis, a Mediterranean chamephyte. Twelve microsatellite loci were characterised and amplified a total of 117 alleles in a sample of 30 individuals from one population, with an average of 9.75 alleles per locus. Observed heterozygosities ranged from 0.333 to 0.900. Cross-species transferability was also assayed in the two other species of the genus. The cumulated probabilities of exclusion for paternity and parentage of the 12 loci were of 0.999971 and 1, respectively, supporting the usefulness of these microsatellite loci for parentage analyses. Nine out of 12 microsatellite loci amplified in the two species and were polymorphic detecting a total of 49 and 45 in R. eriocalyx and R. tomentosus, respectively. Twenty-two alleles were exclusive of R. eriocalyx and 12 of R. tomentosus, additionally, three alleles were shared between these two species but were otherwise absent in the analysed individuals of R. officinalis. In total, this set of markers amplified 154 different microsatellite alleles, supporting their usefulness to conduct population genetic, reproductive biology and hybridisation studies in Rosmarinus. We thank Dr. M. Palop-Esteban for her help during sampling and Prof. J.F. Mota (University of Almería, Spain) for providing samples of R. eriocalyx. This study has been supported by a Project grant "Ayudas para la realización de proyectos de I+D+i para equipos de investigación emergentes o de reciente creación (Ref. GV/2007/259)" from the Conselleria d’Empresa, Universitat i Ciencia, Generalitat Valenciana. J. G. Segarra-Moragues was supported by a Itinerario Integrado de Inserción Profesional (I3P) postdoctoral grant from the Spanish Consejo Superior de Investigaciones Científicas (CSIC) and G. Gleiser was supported by a FPU PhD grant from the Spanish Ministerio de Educación y Ciencia. Peer reviewed 2012-10-26T10:30:43Z 2012-10-26T10:30:43Z 2009-06 artículo http://purl.org/coar/resource_type/c_6501 Conservation Genetics 10(3): 571-575 (2009) 1566-0621 http://hdl.handle.net/10261/58980 10.1007/s10592-008-9572-7 1572-9737 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003339 en http://dx.doi.org/10.1007/s10592-008-9572-7 none Springer
institution CIDE ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-cide-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del CIDE España
language English
topic Labiatae
Rosmarinus
Genetic diversity
SSR
Labiatae
Rosmarinus
Genetic diversity
SSR
spellingShingle Labiatae
Rosmarinus
Genetic diversity
SSR
Labiatae
Rosmarinus
Genetic diversity
SSR
Segarra-Moragues, José G.
Gleiser, G.
Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries
description 5 paginas, 2 tablas.
author2 Generalitat Valenciana
author_facet Generalitat Valenciana
Segarra-Moragues, José G.
Gleiser, G.
format artículo
topic_facet Labiatae
Rosmarinus
Genetic diversity
SSR
author Segarra-Moragues, José G.
Gleiser, G.
author_sort Segarra-Moragues, José G.
title Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries
title_short Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries
title_full Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries
title_fullStr Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries
title_full_unstemmed Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries
title_sort isolation and characterisation of di and tri nucleotide microsatellite loci in rosmarinus officinalis (lamiaceae), using enriched genomic libraries
publisher Springer
publishDate 2009-06
url http://hdl.handle.net/10261/58980
http://dx.doi.org/10.13039/501100003359
http://dx.doi.org/10.13039/501100003339
work_keys_str_mv AT segarramoraguesjoseg isolationandcharacterisationofdiandtrinucleotidemicrosatellitelociinrosmarinusofficinalislamiaceaeusingenrichedgenomiclibraries
AT gleiserg isolationandcharacterisationofdiandtrinucleotidemicrosatellitelociinrosmarinusofficinalislamiaceaeusingenrichedgenomiclibraries
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