Direct Interaction Between NC2 and a Subunit of the SRBs/mediator Co–activator
Transcriptional regulation depends on the appropriate set of positive and negative regulating signals in order to provide the correct gene expression. Studies in eukaryotic gene expression over the last few years have shown that SRBs/Mediator complex is involved in the proper activation of a variety of genes, while NC2 has been shown to be a general repressor of transcription. NC2 is a conserved transcripcional regulator that was originally identified as inhibitor of basal transcription. Here Our initial results show that the repressor NC2 can interact directly with the Mediator in HeLa cells, this interaction provides a new set of interactions between positive and negative cofactors. Furthermore NC2 depletion from HeLa nuclear extract decrease activated transcription from the HIV–LTR1 promoter in vitro but addition of a complex NC2 fraction restores activated transcription. Further dissection showed that NC2 interacts directly with MED21 a subunit of the Mediator complex. The results shown may provide clues to the controversial role that NC2 has on active promoters.
Main Authors: | , , , |
---|---|
Format: | info:eu-repo/semantics/article biblioteca |
Language: | eng |
Subjects: | info:eu-repo/classification/Autores/CO-ACTIVATOR, info:eu-repo/classification/Autores/REPRESSOR, info:eu-repo/classification/Autores/MEDIATOR, info:eu-repo/classification/Autores/MED21, info:eu-repo/classification/Autores/GENE REGULATION, info:eu-repo/classification/cti/2, |
Online Access: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/804 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cicy-1003-804 |
---|---|
record_format |
koha |
spelling |
dig-cicy-1003-8042017-12-09T00:16:28Z Direct Interaction Between NC2 and a Subunit of the SRBs/mediator Co–activator JUAN GUALBERTO COLLI MULL DIANA KARINA GUILLEN MALDONADO LUIS CARLOS RODRIGUEZ ZAPATA ENRIQUE CASTAÑO DE LA SERNA 2006 info:eu-repo/semantics/article Transcriptional regulation depends on the appropriate set of positive and negative regulating signals in order to provide the correct gene expression. Studies in eukaryotic gene expression over the last few years have shown that SRBs/Mediator complex is involved in the proper activation of a variety of genes, while NC2 has been shown to be a general repressor of transcription. NC2 is a conserved transcripcional regulator that was originally identified as inhibitor of basal transcription. Here Our initial results show that the repressor NC2 can interact directly with the Mediator in HeLa cells, this interaction provides a new set of interactions between positive and negative cofactors. Furthermore NC2 depletion from HeLa nuclear extract decrease activated transcription from the HIV–LTR1 promoter in vitro but addition of a complex NC2 fraction restores activated transcription. Further dissection showed that NC2 interacts directly with MED21 a subunit of the Mediator complex. The results shown may provide clues to the controversial role that NC2 has on active promoters. info:eu-repo/classification/Autores/CO-ACTIVATOR info:eu-repo/classification/Autores/REPRESSOR info:eu-repo/classification/Autores/MEDIATOR info:eu-repo/classification/Autores/MED21 info:eu-repo/classification/Autores/GENE REGULATION info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 Asian Journal of Biochemistry, 1(1), 75-83, 2006 http://cicy.repositorioinstitucional.mx/jspui/handle/1003/804 info:eu-repo/semantics/openAccess eng citation:Mobious, N., & Castano, E. (2006). Direct interaction between NC2 and a Subunit of the SRBs/mediator Co-activator. Asian Journal of Biochemistry, 1(1), 75-83. http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf |
institution |
CICY |
collection |
DSpace |
country |
México |
countrycode |
MX |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cicy |
tag |
biblioteca |
region |
America del Norte |
libraryname |
Biblioteca del CICY |
language |
eng |
topic |
info:eu-repo/classification/Autores/CO-ACTIVATOR info:eu-repo/classification/Autores/REPRESSOR info:eu-repo/classification/Autores/MEDIATOR info:eu-repo/classification/Autores/MED21 info:eu-repo/classification/Autores/GENE REGULATION info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/CO-ACTIVATOR info:eu-repo/classification/Autores/REPRESSOR info:eu-repo/classification/Autores/MEDIATOR info:eu-repo/classification/Autores/MED21 info:eu-repo/classification/Autores/GENE REGULATION info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
spellingShingle |
info:eu-repo/classification/Autores/CO-ACTIVATOR info:eu-repo/classification/Autores/REPRESSOR info:eu-repo/classification/Autores/MEDIATOR info:eu-repo/classification/Autores/MED21 info:eu-repo/classification/Autores/GENE REGULATION info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/CO-ACTIVATOR info:eu-repo/classification/Autores/REPRESSOR info:eu-repo/classification/Autores/MEDIATOR info:eu-repo/classification/Autores/MED21 info:eu-repo/classification/Autores/GENE REGULATION info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 JUAN GUALBERTO COLLI MULL DIANA KARINA GUILLEN MALDONADO LUIS CARLOS RODRIGUEZ ZAPATA ENRIQUE CASTAÑO DE LA SERNA Direct Interaction Between NC2 and a Subunit of the SRBs/mediator Co–activator |
description |
Transcriptional regulation depends on the appropriate set of positive and negative regulating signals in order to provide the correct gene expression. Studies in eukaryotic gene expression over the last few years have shown that SRBs/Mediator complex is involved in the proper activation of a variety of genes, while NC2 has been shown to be a general repressor of transcription. NC2 is a conserved transcripcional regulator that was originally identified as inhibitor of basal transcription. Here Our initial results show that the repressor NC2 can interact directly with the Mediator in HeLa cells, this interaction provides a new set of interactions between positive and negative cofactors. Furthermore NC2 depletion from HeLa nuclear extract decrease activated transcription from the HIV–LTR1 promoter in vitro but addition of a complex NC2 fraction restores activated transcription. Further dissection showed that NC2 interacts directly with MED21 a subunit of the Mediator complex. The results shown may provide clues to the controversial role that NC2 has on active promoters. |
format |
info:eu-repo/semantics/article |
topic_facet |
info:eu-repo/classification/Autores/CO-ACTIVATOR info:eu-repo/classification/Autores/REPRESSOR info:eu-repo/classification/Autores/MEDIATOR info:eu-repo/classification/Autores/MED21 info:eu-repo/classification/Autores/GENE REGULATION info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
author |
JUAN GUALBERTO COLLI MULL DIANA KARINA GUILLEN MALDONADO LUIS CARLOS RODRIGUEZ ZAPATA ENRIQUE CASTAÑO DE LA SERNA |
author_facet |
JUAN GUALBERTO COLLI MULL DIANA KARINA GUILLEN MALDONADO LUIS CARLOS RODRIGUEZ ZAPATA ENRIQUE CASTAÑO DE LA SERNA |
author_sort |
JUAN GUALBERTO COLLI MULL |
title |
Direct Interaction Between NC2 and a Subunit of the SRBs/mediator Co–activator |
title_short |
Direct Interaction Between NC2 and a Subunit of the SRBs/mediator Co–activator |
title_full |
Direct Interaction Between NC2 and a Subunit of the SRBs/mediator Co–activator |
title_fullStr |
Direct Interaction Between NC2 and a Subunit of the SRBs/mediator Co–activator |
title_full_unstemmed |
Direct Interaction Between NC2 and a Subunit of the SRBs/mediator Co–activator |
title_sort |
direct interaction between nc2 and a subunit of the srbs/mediator co–activator |
url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/804 |
work_keys_str_mv |
AT juangualbertocollimull directinteractionbetweennc2andasubunitofthesrbsmediatorcoactivator AT dianakarinaguillenmaldonado directinteractionbetweennc2andasubunitofthesrbsmediatorcoactivator AT luiscarlosrodriguezzapata directinteractionbetweennc2andasubunitofthesrbsmediatorcoactivator AT enriquecastanodelaserna directinteractionbetweennc2andasubunitofthesrbsmediatorcoactivator |
_version_ |
1756089423794012160 |