Thidiazuron-induced shoot organogenesis of Cleome viscosa (L) through cotyledonary explants culture

A reproducible protocol for direct shoot organogenesis of Cleome viscosa, an important medicinal weed herb was developed. The seed explants were collected primarily from field grown mature plants for in vitro germination on different strength of MS basal medium with or without selection of GA3 at various concentration (0.1 to 1.0 mg/L). The highest rate of seed germination (55.3%) was noticed on full strength MS basal medium fortified with 0.5 mg/L GA3 after 30 days of culture. The excised 7 to 10 days old cotyledonary leaf, cotyledonary node and hypocotyls explants cultured on MS medium fortified with different concentration of individual cytokinin (BA/KIN/TDZ) alone or BA+KIN or TDZ+KIN or TDZ in combinations with different auxins (IBA/NAA/IAA) influenced the frequency of adventitious microshoot formation.

Bibliographic Details

Main Author:	ENRIQUE CASTAÑO DE LA SERNA
Format:	info:eu-repo/semantics/article biblioteca
Language:	eng
Subjects:	info:eu-repo/classification/Autores/CLEOME VISCOSA L, info:eu-repo/classification/Autores/MEDICINAL WEED PLANT, info:eu-repo/classification/Autores/SHOOT MULTIPLICATION, info:eu-repo/classification/Autores/DIRECT ORGANOGENESIS, info:eu-repo/classification/cti/2,
Online Access:	http://cicy.repositorioinstitucional.mx/jspui/handle/1003/724
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